FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383497985> ?p ?o ?g. }

• W4383497985 endingPage "109414" @default.
• W4383497985 startingPage "109414" @default.
• W4383497985 abstract "The global incidence of nonalcoholic fatty liver disease (NAFLD) has been surging in recent years, however, no drug is currently approved to treat this disease. Quercetin, a natural flavonoid abundant in plants and fruits, has been reported to alleviate NAFLD, however, the exact molecular mechanism remains unclear. This study aims to further elucidate its potential mechanism of action. The beneficial effects and the underlying mechanism of quercetin in alleviating NAFLD were explored both in vitro and in vivo, by employing chemical inhibitors of autophagosomes (3-methyladenine, 3-MA), autolysosomes (chloroquine, CQ), AMPK (Compound C, CC) and SIRT1 (selisistat, EX-527). The levels of intracellular lipids, reactive oxygen species, mitochondria function, autophagy, and mitophagy were assessed by fluorescent labeling and examined using flow cytometry or confocal microscopy. Key protein expressions of autophagy, mitophagy, and inflammation were also determined. In vivo, quercetin was shown to dose-dependently effectively alleviate NAFLD, but intraperitoneal injection of 3-MA could block the beneficial effects of quercetin on body weight, liver weight, serum ALT/AST, hepatic ROS and inflammation. In vitro, quercetin could reduce intracellular lipids (Nile Red staining) and ROS/DHE accumulation, which could be also blocked by 3-MA or CQ. Furthermore, we found that CC could abrogate the protective effects of quercetin on lipid and ROS accumulation in vitro. Also, CC abolished the proautophagic and anti-inflammatory effects of quercetin, as shown by western blot determination and Lyso-Tracker labeling. Importantly, mitophagy, a specific form of mitochondria-targeted autophagy, was enhanced by quercetin, as demonstrated by PINK1/Parkin protein variation and immunofluorescence colocalization of autophagosomes and mitochondria, which could also be blocked by the intervention of CC. This study demonstrates that quercetin prevents NAFLD through AMPK-mediated mitophagy and suggests that promoting mitophagy via an upregulation of AMPK may be a promising therapeutic strategy against NAFLD." @default.
• W4383497985 created "2023-07-08" @default.
• W4383497985 creator A5005228021 @default.
• W4383497985 creator A5007207069 @default.
• W4383497985 creator A5014947716 @default.
• W4383497985 creator A5015195367 @default.
• W4383497985 creator A5017489902 @default.
• W4383497985 creator A5019404357 @default.
• W4383497985 creator A5021621005 @default.
• W4383497985 creator A5029667848 @default.
• W4383497985 creator A5040210858 @default.
• W4383497985 creator A5044083641 @default.
• W4383497985 creator A5074734978 @default.
• W4383497985 creator A5078811566 @default.
• W4383497985 creator A5080566282 @default.
• W4383497985 creator A5085403638 @default.
• W4383497985 date "2023-10-01" @default.
• W4383497985 modified "2023-10-18" @default.
• W4383497985 title "Quercetin ameliorates nonalcoholic fatty liver disease (NAFLD) via the promotion of AMPK-mediated hepatic mitophagy" @default.
• W4383497985 cites W1971155457 @default.
• W4383497985 cites W2046732760 @default.
• W4383497985 cites W2076683315 @default.
• W4383497985 cites W2081840727 @default.
• W4383497985 cites W2115901158 @default.
• W4383497985 cites W2180237189 @default.
• W4383497985 cites W2186746217 @default.
• W4383497985 cites W2241406248 @default.
• W4383497985 cites W2491047136 @default.
• W4383497985 cites W2666814845 @default.
• W4383497985 cites W2789877362 @default.
• W4383497985 cites W2810259446 @default.
• W4383497985 cites W2810601090 @default.
• W4383497985 cites W2839544824 @default.
• W4383497985 cites W2883041905 @default.
• W4383497985 cites W2887998229 @default.
• W4383497985 cites W2900302857 @default.
• W4383497985 cites W2906521953 @default.
• W4383497985 cites W3004878341 @default.
• W4383497985 cites W3020404451 @default.
• W4383497985 cites W3038745441 @default.
• W4383497985 cites W3111458415 @default.
• W4383497985 cites W3117083078 @default.
• W4383497985 cites W3156744626 @default.
• W4383497985 cites W3158550233 @default.
• W4383497985 cites W3164013636 @default.
• W4383497985 cites W3176932814 @default.
• W4383497985 cites W3200847517 @default.
• W4383497985 cites W3208293484 @default.
• W4383497985 cites W3212928204 @default.
• W4383497985 cites W4200282703 @default.
• W4383497985 cites W4205252408 @default.
• W4383497985 cites W4285808973 @default.
• W4383497985 cites W4313237264 @default.
• W4383497985 doi "https://doi.org/10.1016/j.jnutbio.2023.109414" @default.
• W4383497985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37423322" @default.
• W4383497985 hasPublicationYear "2023" @default.
• W4383497985 type Work @default.
• W4383497985 citedByCount "2" @default.
• W4383497985 countsByYear W43834979852023 @default.
• W4383497985 crossrefType "journal-article" @default.
• W4383497985 hasAuthorship W4383497985A5005228021 @default.
• W4383497985 hasAuthorship W4383497985A5007207069 @default.
• W4383497985 hasAuthorship W4383497985A5014947716 @default.
• W4383497985 hasAuthorship W4383497985A5015195367 @default.
• W4383497985 hasAuthorship W4383497985A5017489902 @default.
• W4383497985 hasAuthorship W4383497985A5019404357 @default.
• W4383497985 hasAuthorship W4383497985A5021621005 @default.
• W4383497985 hasAuthorship W4383497985A5029667848 @default.
• W4383497985 hasAuthorship W4383497985A5040210858 @default.
• W4383497985 hasAuthorship W4383497985A5044083641 @default.
• W4383497985 hasAuthorship W4383497985A5074734978 @default.
• W4383497985 hasAuthorship W4383497985A5078811566 @default.
• W4383497985 hasAuthorship W4383497985A5080566282 @default.
• W4383497985 hasAuthorship W4383497985A5085403638 @default.
• W4383497985 hasConcept C126322002 @default.
• W4383497985 hasConcept C181199279 @default.
• W4383497985 hasConcept C185592680 @default.
• W4383497985 hasConcept C190283241 @default.
• W4383497985 hasConcept C203522944 @default.
• W4383497985 hasConcept C2776954865 @default.
• W4383497985 hasConcept C2777229759 @default.
• W4383497985 hasConcept C2778004101 @default.
• W4383497985 hasConcept C2778772119 @default.
• W4383497985 hasConcept C2778918659 @default.
• W4383497985 hasConcept C2779134260 @default.
• W4383497985 hasConcept C2779324830 @default.
• W4383497985 hasConcept C2779934242 @default.
• W4383497985 hasConcept C2780124434 @default.
• W4383497985 hasConcept C2781263971 @default.
• W4383497985 hasConcept C28859421 @default.
• W4383497985 hasConcept C41625074 @default.
• W4383497985 hasConcept C55493867 @default.
• W4383497985 hasConcept C71924100 @default.
• W4383497985 hasConcept C86803240 @default.
• W4383497985 hasConcept C97029542 @default.
• W4383497985 hasConcept C98274493 @default.
• W4383497985 hasConceptScore W4383497985C126322002 @default.
• W4383497985 hasConceptScore W4383497985C181199279 @default.

• next