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- W4383500622 abstract "Phenotypic methods relying on colony morphology, microscopic observation, and in some instances biochemical reactions, remain the mainstay of fungal identification. However, molecular methods are increasingly being used for the detection and/or differentiation of medically important fungi. A considerable number of laboratory-developed real-time or endpoint polymerase chain reaction techniques have been developed for the identification of fungi and are reported in peer-reviewed literature. Matrix-assisted laser desorption ionization–time of flight mass spectrometry is a protein-based method that has revolutionized the identification of microorganisms in clinical microbiology, including fungi. Non-sequencing nucleic acid-based methods may be separated into signal amplification methods and nucleic acid amplification methods. Sanger sequencing is the most common DNA sequencing method used in clinical laboratories today. Massive parallel sequencing is a DNA sequencing technology similar in principle to Sanger sequencing in that it provides DNA sequence information by synthesis of the target DNA. The chapter describes the uses for nucleotide sequence information of an isolate." @default.
- W4383500622 created "2023-07-08" @default.
- W4383500622 date "2023-07-07" @default.
- W4383500622 modified "2023-10-18" @default.
- W4383500622 title "Introduction" @default.
- W4383500622 doi "https://doi.org/10.1002/9781683674436.part3" @default.
- W4383500622 hasPublicationYear "2023" @default.
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