Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383500900> ?p ?o ?g. }
- W4383500900 endingPage "104946" @default.
- W4383500900 startingPage "104946" @default.
- W4383500900 abstract "Spores/pollen records abundant information of biodiversity, evolution, and climate condition. The exine of spores/pollen is mainly composed of sporopollenin, which is chemically stable over geological time. Morphological study of the exine is a classical method used in palynology. In this study, we developed a fluorescence lifetime imaging (FLIM) method to investigate the numbers and lifetimes of fluorophores in sporopollenin. We used 20 pollen grains of each species to study sporopollenin FLIM. The sporopollenin was purified using the acetolysis method. Laser scanning confocal microscopy (LSCM) was used to study the autofluorescence of sporopollenin. The sporopollenin FLIM of pollen grains collected in different years/geographical populations showed no significant difference. Three fluorophores were estimated in the sporopollenin of all the tested plants, including angiosperms (Oryza sativa and Zea mays, Brassica napus and Arabidopsis thaliana), gymnosperms (Cryptomeria fortune and Metasequoia glyptostroboides), ferns (Lygodium japonicum and Ceratopteris thalictroides), and lycophyte (Lycopodium clavatum). Quantitative analysis demonstrated that the lifetimes of fluorophores varied in different species, showing that FLIM provides additional information to distinguish spores/pollen. Further analysis showed significant differences in sporopollenin fluorescence lifetimes between morphologically similar pollen grains of cultivated crops and their wild relatives of the genera Oryza and Triticum. These results show that sporopollenin FLIM offers a new dimension of information on spores/pollen. This new technique has a big potential to fill a gap for traditional morphological comparison to identify dispersed fossil pollen and spores." @default.
- W4383500900 created "2023-07-08" @default.
- W4383500900 creator A5006673047 @default.
- W4383500900 creator A5035869145 @default.
- W4383500900 creator A5037899452 @default.
- W4383500900 creator A5040235604 @default.
- W4383500900 creator A5059030289 @default.
- W4383500900 creator A5060373466 @default.
- W4383500900 date "2023-09-01" @default.
- W4383500900 modified "2023-10-14" @default.
- W4383500900 title "Fluorescence lifetime imaging of sporopollenin: An alternative way to improve taxonomic level of identifying dispersed pollen and spores" @default.
- W4383500900 cites W1149862443 @default.
- W4383500900 cites W1960176772 @default.
- W4383500900 cites W1967706905 @default.
- W4383500900 cites W1972374522 @default.
- W4383500900 cites W1978417727 @default.
- W4383500900 cites W1981623722 @default.
- W4383500900 cites W1994232557 @default.
- W4383500900 cites W1998506149 @default.
- W4383500900 cites W1999781521 @default.
- W4383500900 cites W2001633008 @default.
- W4383500900 cites W2008398279 @default.
- W4383500900 cites W2009256513 @default.
- W4383500900 cites W2013277649 @default.
- W4383500900 cites W2015683351 @default.
- W4383500900 cites W2031377957 @default.
- W4383500900 cites W2035202529 @default.
- W4383500900 cites W2037488292 @default.
- W4383500900 cites W2040596609 @default.
- W4383500900 cites W2045272193 @default.
- W4383500900 cites W2062261713 @default.
- W4383500900 cites W2062675899 @default.
- W4383500900 cites W2062709533 @default.
- W4383500900 cites W2069935261 @default.
- W4383500900 cites W2085446806 @default.
- W4383500900 cites W2088944806 @default.
- W4383500900 cites W2097193537 @default.
- W4383500900 cites W2101891826 @default.
- W4383500900 cites W2104662205 @default.
- W4383500900 cites W2109349038 @default.
- W4383500900 cites W2132690534 @default.
- W4383500900 cites W2132857973 @default.
- W4383500900 cites W2140533725 @default.
- W4383500900 cites W2144351682 @default.
- W4383500900 cites W2180821508 @default.
- W4383500900 cites W2203610634 @default.
- W4383500900 cites W2300150155 @default.
- W4383500900 cites W2333574083 @default.
- W4383500900 cites W2508457726 @default.
- W4383500900 cites W2524001927 @default.
- W4383500900 cites W2559719271 @default.
- W4383500900 cites W2604635959 @default.
- W4383500900 cites W2737709875 @default.
- W4383500900 cites W2760249415 @default.
- W4383500900 cites W2767873902 @default.
- W4383500900 cites W2946537211 @default.
- W4383500900 cites W2953161691 @default.
- W4383500900 cites W2999246298 @default.
- W4383500900 cites W3003716196 @default.
- W4383500900 cites W3013608099 @default.
- W4383500900 cites W3049478358 @default.
- W4383500900 cites W3094439952 @default.
- W4383500900 cites W3168928724 @default.
- W4383500900 cites W3198448078 @default.
- W4383500900 cites W4231195967 @default.
- W4383500900 cites W4313706706 @default.
- W4383500900 cites W4320731751 @default.
- W4383500900 doi "https://doi.org/10.1016/j.revpalbo.2023.104946" @default.
- W4383500900 hasPublicationYear "2023" @default.
- W4383500900 type Work @default.
- W4383500900 citedByCount "0" @default.
- W4383500900 crossrefType "journal-article" @default.
- W4383500900 hasAuthorship W4383500900A5006673047 @default.
- W4383500900 hasAuthorship W4383500900A5035869145 @default.
- W4383500900 hasAuthorship W4383500900A5037899452 @default.
- W4383500900 hasAuthorship W4383500900A5040235604 @default.
- W4383500900 hasAuthorship W4383500900A5059030289 @default.
- W4383500900 hasAuthorship W4383500900A5060373466 @default.
- W4383500900 hasConcept C162501224 @default.
- W4383500900 hasConcept C167196801 @default.
- W4383500900 hasConcept C174618031 @default.
- W4383500900 hasConcept C2780618852 @default.
- W4383500900 hasConcept C59822182 @default.
- W4383500900 hasConcept C86803240 @default.
- W4383500900 hasConceptScore W4383500900C162501224 @default.
- W4383500900 hasConceptScore W4383500900C167196801 @default.
- W4383500900 hasConceptScore W4383500900C174618031 @default.
- W4383500900 hasConceptScore W4383500900C2780618852 @default.
- W4383500900 hasConceptScore W4383500900C59822182 @default.
- W4383500900 hasConceptScore W4383500900C86803240 @default.
- W4383500900 hasFunder F4320321001 @default.
- W4383500900 hasFunder F4320321133 @default.
- W4383500900 hasFunder F4320327534 @default.
- W4383500900 hasLocation W43835009001 @default.
- W4383500900 hasOpenAccess W4383500900 @default.
- W4383500900 hasPrimaryLocation W43835009001 @default.
- W4383500900 hasRelatedWork W1974778068 @default.
- W4383500900 hasRelatedWork W1976741903 @default.