Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383551208> ?p ?o ?g. }
- W4383551208 endingPage "11166" @default.
- W4383551208 startingPage "11166" @default.
- W4383551208 abstract "The interplay between inflammatory and redox processes is a ubiquitous and critical phenomenon in cell biology that involves numerous biological factors. Among them, secretory phospholipases A2 (sPLA2) that catalyze the hydrolysis of the sn-2 ester bond of phospholipids are key players. They can interact or be modulated by the presence of truncated oxidized phosphatidylcholines (OxPCs) produced under oxidative stress from phosphatidylcholine (PC) species. The present study examined this important, but rarely considered, sPLA2 modulation induced by the changes in biophysical properties of PC vesicles comprising various OxPC ratios in mono- or poly-unsaturated PCs. Being the most physiologically active OxPCs, 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) and 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) have been selected for our study. Using fluorescence spectroscopy methods, we compared the effect of OxPCs on the lipid order as well as sPLA2 activity in large unilamellar vesicles (LUVs) made of the heteroacid PC, either monounsaturated [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)], or polyunsaturated [1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDPC)] at a physiological temperature. The effect of OxPCs on vesicle size was also assessed in both the mono- and polyunsaturated PC matrices. Results: OxPCs decrease the membrane lipid order of POPC and PDPC mixtures with PGPC inducing a much larger decrease in comparison with POVPC, indicative that the difference takes place at the glycerol level. Compared with POPC, PDPC was able to inhibit sPLA2 activity showing a protective effect of PDPC against enzyme hydrolysis. Furthermore, sPLA2 activity on its PC substrates was modulated by the OxPC membrane content. POVPC down-regulated sPLA2 activity, suggesting anti-inflammatory properties of this truncated oxidized lipid. Interestingly, PGPC had a dual and opposite effect, either inhibitory or enhancing on sPLA2 activity, depending on the protocol of lipid mixing. This difference may result from the chemical properties of the shortened sn-2-acyl chain residues (aldehyde group for POVPC, and carboxyl for PGPC), being, respectively, zwitterionic or anionic under hydration at physiological conditions." @default.
- W4383551208 created "2023-07-08" @default.
- W4383551208 creator A5001625655 @default.
- W4383551208 creator A5002092920 @default.
- W4383551208 creator A5006151008 @default.
- W4383551208 creator A5013948005 @default.
- W4383551208 creator A5067449194 @default.
- W4383551208 creator A5068840687 @default.
- W4383551208 creator A5070868562 @default.
- W4383551208 creator A5073479497 @default.
- W4383551208 creator A5082314364 @default.
- W4383551208 creator A5089612398 @default.
- W4383551208 date "2023-07-06" @default.
- W4383551208 modified "2023-09-25" @default.
- W4383551208 title "Impact of Truncated Oxidized Phosphatidylcholines on Phospholipase A2 Activity in Mono- and Polyunsaturated Biomimetic Vesicles" @default.
- W4383551208 cites W12246812 @default.
- W4383551208 cites W1229372959 @default.
- W4383551208 cites W1482031494 @default.
- W4383551208 cites W1734082729 @default.
- W4383551208 cites W1964991966 @default.
- W4383551208 cites W1966217960 @default.
- W4383551208 cites W1966556984 @default.
- W4383551208 cites W1968008704 @default.
- W4383551208 cites W1969043866 @default.
- W4383551208 cites W1971677534 @default.
- W4383551208 cites W1972701314 @default.
- W4383551208 cites W1977216019 @default.
- W4383551208 cites W1982278900 @default.
- W4383551208 cites W1982375207 @default.
- W4383551208 cites W1985593949 @default.
- W4383551208 cites W1992648442 @default.
- W4383551208 cites W1997708774 @default.
- W4383551208 cites W1998534538 @default.
- W4383551208 cites W2003815276 @default.
- W4383551208 cites W2004313693 @default.
- W4383551208 cites W2007250945 @default.
- W4383551208 cites W2009559464 @default.
- W4383551208 cites W2014104927 @default.
- W4383551208 cites W2017535823 @default.
- W4383551208 cites W2033048067 @default.
- W4383551208 cites W2033259569 @default.
- W4383551208 cites W2038434242 @default.
- W4383551208 cites W2039930256 @default.
- W4383551208 cites W2044832401 @default.
- W4383551208 cites W2045772297 @default.
- W4383551208 cites W2048550135 @default.
- W4383551208 cites W2049836259 @default.
- W4383551208 cites W2063116304 @default.
- W4383551208 cites W2063630296 @default.
- W4383551208 cites W2064287832 @default.
- W4383551208 cites W2067217087 @default.
- W4383551208 cites W2068006123 @default.
- W4383551208 cites W2076918344 @default.
- W4383551208 cites W2077755739 @default.
- W4383551208 cites W2078255669 @default.
- W4383551208 cites W2079884888 @default.
- W4383551208 cites W2083681924 @default.
- W4383551208 cites W2112799653 @default.
- W4383551208 cites W2113065520 @default.
- W4383551208 cites W2119818848 @default.
- W4383551208 cites W2123918140 @default.
- W4383551208 cites W2126408606 @default.
- W4383551208 cites W2134723427 @default.
- W4383551208 cites W2135099183 @default.
- W4383551208 cites W2137511741 @default.
- W4383551208 cites W2138695494 @default.
- W4383551208 cites W2153400793 @default.
- W4383551208 cites W2153449715 @default.
- W4383551208 cites W2156613610 @default.
- W4383551208 cites W2156831614 @default.
- W4383551208 cites W2157789119 @default.
- W4383551208 cites W2158431134 @default.
- W4383551208 cites W2161456325 @default.
- W4383551208 cites W2165201727 @default.
- W4383551208 cites W2168169159 @default.
- W4383551208 cites W2176425068 @default.
- W4383551208 cites W2273992258 @default.
- W4383551208 cites W2494720203 @default.
- W4383551208 cites W2562095118 @default.
- W4383551208 cites W2564701131 @default.
- W4383551208 cites W2765644150 @default.
- W4383551208 cites W2888330068 @default.
- W4383551208 cites W2899502823 @default.
- W4383551208 cites W3091549776 @default.
- W4383551208 cites W3191916714 @default.
- W4383551208 cites W40092233 @default.
- W4383551208 cites W4244311005 @default.
- W4383551208 cites W4255664210 @default.
- W4383551208 cites W4308112123 @default.
- W4383551208 cites W588180228 @default.
- W4383551208 doi "https://doi.org/10.3390/ijms241311166" @default.
- W4383551208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37446342" @default.
- W4383551208 hasPublicationYear "2023" @default.
- W4383551208 type Work @default.
- W4383551208 citedByCount "0" @default.
- W4383551208 crossrefType "journal-article" @default.
- W4383551208 hasAuthorship W4383551208A5001625655 @default.
- W4383551208 hasAuthorship W4383551208A5002092920 @default.