FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383559207> ?p ?o ?g. }

• W4383559207 endingPage "102926" @default.
• W4383559207 startingPage "102926" @default.
• W4383559207 abstract "Since 2010, the Duck Tembusu virus (DTMUV) has caused a severe outbreak of egg drop syndrome in laying ducks in China, which has resulted in substantial financial losses in the poultry industry. DTMUV nonstructural protein 1 (NS1), as the only secreted protein, could aid in the development of therapeutic antibodies and diagnostic techniques, however, there are few studies on the preparation and epitope identification of monoclonal antibodies (mAbs) against DTMUV NS1. In this study, by indirect enzyme-linked immunosorbent assay (ELISA), western blotting and indirect immunofluorescence assay, we screened six mAbs (8A4, 8E6, 10F12, 1H11, 3D5, 5C11) that could specifically recognize DTMUV NS1. For epitope mapping of mAbs, a series of GST-tagged truncated fusion proteins of DTMUV NS1 were constructed by prokaryotic expression. Finally, the four shortest linear epitopes were identified by indirect ELISA and western blotting. The epitope 133FVIDGPK139 was recognized by 8A4, the epitope 243IPKTLGGP250 was recognized by 8E6, the epitope 267PWDEK271 was recognized by 10F12 and 156EDFGFGVL163 was recognized by 1H11, 3D5 and 5C11. By sequence alignment and cross-reaction tests, we found that 8A4 and 8E6 had high specificity for DTMUV NS1 compared with that of other mAbs, but 10F12, 1H11, 3D5 and 5C11 exhibited a clear degree of cross-reaction with dengue virus (DENV), Japanese encephalitis virus (JEV), West Nile virus (WNV) and Zika virus (ZIKV) NS1. Finally, the predicted crystal structure analysis showed the approximate spatial positions of the four epitopes on the NS1 dimer. In summary, our study revealed two specific mAbs for DTMUV NS1 recognition and four multiflavivirus mAbs for DENV, JEV, WNV and ZIKV NS1 recognition." @default.
• W4383559207 created "2023-07-08" @default.
• W4383559207 creator A5000460901 @default.
• W4383559207 creator A5006232767 @default.
• W4383559207 creator A5007648400 @default.
• W4383559207 creator A5013454053 @default.
• W4383559207 creator A5021517739 @default.
• W4383559207 creator A5031331731 @default.
• W4383559207 creator A5035312767 @default.
• W4383559207 creator A5039291898 @default.
• W4383559207 creator A5040485279 @default.
• W4383559207 creator A5045418032 @default.
• W4383559207 creator A5049672306 @default.
• W4383559207 creator A5051279926 @default.
• W4383559207 creator A5051889783 @default.
• W4383559207 creator A5063829017 @default.
• W4383559207 creator A5064308879 @default.
• W4383559207 creator A5075014478 @default.
• W4383559207 creator A5076996063 @default.
• W4383559207 creator A5083684928 @default.
• W4383559207 creator A5086086173 @default.
• W4383559207 creator A5088124858 @default.
• W4383559207 date "2023-10-01" @default.
• W4383559207 modified "2023-10-16" @default.
• W4383559207 title "Linear epitope identification of monoclonal antibodies against the duck Tembusu virus NS1" @default.
• W4383559207 cites W1976308168 @default.
• W4383559207 cites W2098136541 @default.
• W4383559207 cites W2141375220 @default.
• W4383559207 cites W2167041942 @default.
• W4383559207 cites W2172031283 @default.
• W4383559207 cites W2178559328 @default.
• W4383559207 cites W2187937073 @default.
• W4383559207 cites W2301807445 @default.
• W4383559207 cites W2302414979 @default.
• W4383559207 cites W2346452525 @default.
• W4383559207 cites W2606996549 @default.
• W4383559207 cites W2612196570 @default.
• W4383559207 cites W2753223918 @default.
• W4383559207 cites W2755457901 @default.
• W4383559207 cites W2765619750 @default.
• W4383559207 cites W2798042901 @default.
• W4383559207 cites W2810530231 @default.
• W4383559207 cites W2931187776 @default.
• W4383559207 cites W2939799025 @default.
• W4383559207 cites W3093994474 @default.
• W4383559207 cites W3119876490 @default.
• W4383559207 cites W3120974536 @default.
• W4383559207 cites W3127456009 @default.
• W4383559207 cites W3154893391 @default.
• W4383559207 cites W3166525475 @default.
• W4383559207 cites W3196369861 @default.
• W4383559207 cites W3205027310 @default.
• W4383559207 cites W3216209897 @default.
• W4383559207 cites W4304005703 @default.
• W4383559207 cites W4321373643 @default.
• W4383559207 cites W4324139704 @default.
• W4383559207 doi "https://doi.org/10.1016/j.psj.2023.102926" @default.
• W4383559207 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37499611" @default.
• W4383559207 hasPublicationYear "2023" @default.
• W4383559207 type Work @default.
• W4383559207 citedByCount "0" @default.
• W4383559207 crossrefType "journal-article" @default.
• W4383559207 hasAuthorship W4383559207A5000460901 @default.
• W4383559207 hasAuthorship W4383559207A5006232767 @default.
• W4383559207 hasAuthorship W4383559207A5007648400 @default.
• W4383559207 hasAuthorship W4383559207A5013454053 @default.
• W4383559207 hasAuthorship W4383559207A5021517739 @default.
• W4383559207 hasAuthorship W4383559207A5031331731 @default.
• W4383559207 hasAuthorship W4383559207A5035312767 @default.
• W4383559207 hasAuthorship W4383559207A5039291898 @default.
• W4383559207 hasAuthorship W4383559207A5040485279 @default.
• W4383559207 hasAuthorship W4383559207A5045418032 @default.
• W4383559207 hasAuthorship W4383559207A5049672306 @default.
• W4383559207 hasAuthorship W4383559207A5051279926 @default.
• W4383559207 hasAuthorship W4383559207A5051889783 @default.
• W4383559207 hasAuthorship W4383559207A5063829017 @default.
• W4383559207 hasAuthorship W4383559207A5064308879 @default.
• W4383559207 hasAuthorship W4383559207A5075014478 @default.
• W4383559207 hasAuthorship W4383559207A5076996063 @default.
• W4383559207 hasAuthorship W4383559207A5083684928 @default.
• W4383559207 hasAuthorship W4383559207A5086086173 @default.
• W4383559207 hasAuthorship W4383559207A5088124858 @default.
• W4383559207 hasBestOaLocation W43835592071 @default.
• W4383559207 hasConcept C159047783 @default.
• W4383559207 hasConcept C159654299 @default.
• W4383559207 hasConcept C195616568 @default.
• W4383559207 hasConcept C203014093 @default.
• W4383559207 hasConcept C2522874641 @default.
• W4383559207 hasConcept C2779635636 @default.
• W4383559207 hasConcept C2780568436 @default.
• W4383559207 hasConcept C2780632150 @default.
• W4383559207 hasConcept C2781241564 @default.
• W4383559207 hasConcept C542903549 @default.
• W4383559207 hasConcept C78598654 @default.
• W4383559207 hasConcept C86803240 @default.
• W4383559207 hasConceptScore W4383559207C159047783 @default.
• W4383559207 hasConceptScore W4383559207C159654299 @default.
• W4383559207 hasConceptScore W4383559207C195616568 @default.

• next