Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383561311> ?p ?o ?g. }
- W4383561311 endingPage "561" @default.
- W4383561311 startingPage "550" @default.
- W4383561311 abstract "Abstract— An efficient system for the biosynthesis, isolation and purification of recombinant human neuroglobin has been developed and optimized, which makes it possible to produce protein in quantities sufficient to study its properties. According to UV-visible, IR-, CD-, and NMR spectroscopy data, recombinant neuroglobin is a structured protein in the holoform state. The data of chromato-mass-spectrometric analysis made it possible to conclude that there is a correctly formed disulfide bond in the structure of the oxidized form of the protein. Using Raman and surface-enhanced Raman spectroscopy with laser excitation at 532 nm, it was shown that heme in the reduced and oxidized forms of neuroglobin has vibrational degrees of freedom typical of b -type hemes, and the iron atom is hexacoordinated. Using Raman spectroscopy with laser excitation at 633 nm, it was found that reduced –SH-groups were present in reduced neuroglobin, while in oxidized neuroglobin a disulfide bridge was formed. The results obtained serve as the basis for detailed studies of the functioning of neuroglobin as a neuroprotector, in particular, during its interaction with oxidized cytochrome c , which is released from mitochondria in violation of their functioning and/or morphology." @default.
- W4383561311 created "2023-07-08" @default.
- W4383561311 creator A5001226147 @default.
- W4383561311 creator A5003000801 @default.
- W4383561311 creator A5003536760 @default.
- W4383561311 creator A5019942894 @default.
- W4383561311 creator A5020234744 @default.
- W4383561311 creator A5051228467 @default.
- W4383561311 creator A5055846192 @default.
- W4383561311 creator A5064424486 @default.
- W4383561311 creator A5071254410 @default.
- W4383561311 creator A5074735071 @default.
- W4383561311 creator A5075010755 @default.
- W4383561311 creator A5087735016 @default.
- W4383561311 date "2023-06-01" @default.
- W4383561311 modified "2023-09-27" @default.
- W4383561311 title "Development of a System for Biosynthesis, Isolation and Purification of the Holoform of Recombinant Human Neuroglobin and Its Characteristics" @default.
- W4383561311 cites W1507703835 @default.
- W4383561311 cites W1524781921 @default.
- W4383561311 cites W1530729903 @default.
- W4383561311 cites W1653986885 @default.
- W4383561311 cites W1835198623 @default.
- W4383561311 cites W1974297725 @default.
- W4383561311 cites W1986105950 @default.
- W4383561311 cites W1989314193 @default.
- W4383561311 cites W1991430178 @default.
- W4383561311 cites W2009844268 @default.
- W4383561311 cites W2018421806 @default.
- W4383561311 cites W2021017431 @default.
- W4383561311 cites W2024640096 @default.
- W4383561311 cites W2027438573 @default.
- W4383561311 cites W2032466859 @default.
- W4383561311 cites W2034677778 @default.
- W4383561311 cites W2034762716 @default.
- W4383561311 cites W2051405783 @default.
- W4383561311 cites W2071153202 @default.
- W4383561311 cites W2073103652 @default.
- W4383561311 cites W2078787785 @default.
- W4383561311 cites W2080725398 @default.
- W4383561311 cites W2083043354 @default.
- W4383561311 cites W2083809966 @default.
- W4383561311 cites W2086187832 @default.
- W4383561311 cites W2094395417 @default.
- W4383561311 cites W2104571730 @default.
- W4383561311 cites W2105300484 @default.
- W4383561311 cites W2141135411 @default.
- W4383561311 cites W2143149723 @default.
- W4383561311 cites W2159254132 @default.
- W4383561311 cites W2159426997 @default.
- W4383561311 cites W2164923948 @default.
- W4383561311 cites W2168948442 @default.
- W4383561311 cites W2544717163 @default.
- W4383561311 cites W2575712535 @default.
- W4383561311 cites W2765178386 @default.
- W4383561311 cites W3216888141 @default.
- W4383561311 cites W4225426183 @default.
- W4383561311 cites W4229005316 @default.
- W4383561311 cites W78586248 @default.
- W4383561311 doi "https://doi.org/10.1134/s1068162023030196" @default.
- W4383561311 hasPublicationYear "2023" @default.
- W4383561311 type Work @default.
- W4383561311 citedByCount "0" @default.
- W4383561311 crossrefType "journal-article" @default.
- W4383561311 hasAuthorship W4383561311A5001226147 @default.
- W4383561311 hasAuthorship W4383561311A5003000801 @default.
- W4383561311 hasAuthorship W4383561311A5003536760 @default.
- W4383561311 hasAuthorship W4383561311A5019942894 @default.
- W4383561311 hasAuthorship W4383561311A5020234744 @default.
- W4383561311 hasAuthorship W4383561311A5051228467 @default.
- W4383561311 hasAuthorship W4383561311A5055846192 @default.
- W4383561311 hasAuthorship W4383561311A5064424486 @default.
- W4383561311 hasAuthorship W4383561311A5071254410 @default.
- W4383561311 hasAuthorship W4383561311A5074735071 @default.
- W4383561311 hasAuthorship W4383561311A5075010755 @default.
- W4383561311 hasAuthorship W4383561311A5087735016 @default.
- W4383561311 hasBestOaLocation W43835613111 @default.
- W4383561311 hasConcept C104317684 @default.
- W4383561311 hasConcept C120665830 @default.
- W4383561311 hasConcept C121332964 @default.
- W4383561311 hasConcept C12554922 @default.
- W4383561311 hasConcept C125996951 @default.
- W4383561311 hasConcept C181199279 @default.
- W4383561311 hasConcept C185592680 @default.
- W4383561311 hasConcept C2776217839 @default.
- W4383561311 hasConcept C2776318716 @default.
- W4383561311 hasConcept C32891209 @default.
- W4383561311 hasConcept C40003534 @default.
- W4383561311 hasConcept C40767141 @default.
- W4383561311 hasConcept C553450214 @default.
- W4383561311 hasConcept C55493867 @default.
- W4383561311 hasConcept C62520636 @default.
- W4383561311 hasConcept C75473681 @default.
- W4383561311 hasConcept C8010536 @default.
- W4383561311 hasConcept C86803240 @default.
- W4383561311 hasConceptScore W4383561311C104317684 @default.
- W4383561311 hasConceptScore W4383561311C120665830 @default.
- W4383561311 hasConceptScore W4383561311C121332964 @default.
- W4383561311 hasConceptScore W4383561311C12554922 @default.