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• W4383711277 abstract "Small angle neutron scattering (SANS) measurements are reported for DNA gels under near physiological conditions in which the concentration of monovalent and divalent counter-ions and the pH are varied. The scattering intensity I(q) is described by a two-term equation, one due to osmotic concentration fluctuations and the other coming from static inhomogeneities frozen in by the cross-links. SANS in the low q range indicates the presence of large clusters and the size of which exceeds the resolution of the experiment. In the intermediate q-range, the intensity increases with the CaCl2 concentration and the slope approaches -1, corresponding to linear (rod-like) scatterers. In the highest q region, the scattering response is governed by the local chain geometry. Screening of electrostatic interactions by sodium chloride causes a moderate increase in the SANS intensity that is accompanied by an increase in the mesh size L of the network. Addition of calcium chloride, or a decrease in pH, produces similar trends, and ultimately leads to phase separation. The scattering intensity at q = 0, estimated from independent measurements of the osmotic pressure Π, is in excellent agreement with I(0) from the SANS measurements. Anomalous small angle X-ray scattering (ASAXS) measurements on the uncross-linked DNA show that the monovalent ion cloud is only weakly influenced by the addition of divalent ions. Conversely, the divalent counter-ion cloud tightly follows the contour of polymer chains." @default.
• W4383711277 created "2023-07-11" @default.
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• W4383711277 date "2023-01-01" @default.
• W4383711277 modified "2023-09-28" @default.
• W4383711277 title "Ion-induced changes in DNA gels" @default.
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• W4383711277 doi "https://doi.org/10.1039/d3sm00666b" @default.
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