FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4383817004> ?p ?o ?g. }

• W4383817004 abstract "Background: The prostate gland is surrounded by periprostatic adipose tissue (PPAT) that can release mediators that interfere in prostate function. In this study, we examined the effect of periprostatic adipose tissue supernatant obtained from obese mice on prostate reactivity in vitro and on the viability of human prostatic epithelial cell lines. Methods: Male C57BL/6 mice were fed a standard or high-fat diet after which PPAT was isolated, incubated in Krebs-Henseleit solution for 30 min (without prostate) or 60 min (with prostate), and the supernatant was then collected and screened for biological activity. Total nitrate and nitrite (NOx-) and adenosine were quantified, and the supernatant was then collected and screened for biological activity. NOx- and adenosine were quantified. Concentration-response curves to phenylephrine (PE) were obtained in prostatic tissue from lean and obese mice incubated with or without periprostatic adipose tissue. In some experiments, periprostatic adipose tissue was co-incubated with inhibitors of the nitric oxide (NO)-cyclic guanosine monophosphate pathway (L-NAME, 1400W, ODQ), adenylate cyclase (SQ22536) or with adenosine A2A (ZM241385), and A2B (MRS1754) receptor antagonists. PNT1-A (normal) and BPH-1 (hyperplasic) human epithelial cells were cultured and incubated with supernatant from periprostatic adipose tissue for 24, 48, or 72 h in the absence or presence of these inhibitors/antagonists, after which cell viability and proliferation were assessed. Results: The levels of NOx- and adenosine were significantly higher in the periprostatic adipose tissue supernatant (30 min, without prostate) when compared to the vehicle. A trend toward an increase in the levels of NOX was observed after 60 min. PPAT supernatant from obese mice significantly reduced the PE-induced contractions only in prostate from obese mice. The co-incubation of periprostatic adipose tissue with L-NAME, 1400W, ODQ, or ZM241385 attenuated the anticontractile activity of the periprostatic adipose tissue supernatant. Incubation with the supernatant of periprostatic adipose tissue from obese mice significantly increased the viability of PNT1-A cells and attenuated expression of the apoptosis marker protein caspase-3 when compared to cells incubated with periprostatic adipose tissue from lean mice. Hyperplastic cells (BPH-1) incubated with periprostatic adipose tissue from obese mice showed greater proliferation after 24 h, 48 h, and 72 h compared to cells incubated with culture medium alone. BPH-1 cell proliferation in the presence of PPAT supernatant was attenuated by NO-signaling pathway inhibitors and by adenosine receptor antagonists after 72 h. Conclusion: NO and adenosine are involved in the anticontractile and pro-proliferative activities of periprostatic adipose tissue supernatant from obese mice. More studies are needed to determine whether the blockade of NO and/or adenosine derived from periprostatic adipose tissue can improve prostate function." @default.
• W4383817004 created "2023-07-11" @default.
• W4383817004 creator A5008687370 @default.
• W4383817004 creator A5010015599 @default.
• W4383817004 creator A5011376800 @default.
• W4383817004 creator A5019982577 @default.
• W4383817004 creator A5022015826 @default.
• W4383817004 creator A5023349336 @default.
• W4383817004 creator A5040711249 @default.
• W4383817004 creator A5041617090 @default.
• W4383817004 creator A5060879543 @default.
• W4383817004 creator A5068560871 @default.
• W4383817004 creator A5072937786 @default.
• W4383817004 creator A5091139356 @default.
• W4383817004 date "2023-07-10" @default.
• W4383817004 modified "2023-10-18" @default.
• W4383817004 title "Periprostatic adipose tissue (PPAT) supernatant from obese mice releases anticontractile substances and increases human prostate epithelial cell proliferation: the role of nitric oxide and adenosine" @default.
• W4383817004 cites W1678115652 @default.
• W4383817004 cites W1973678472 @default.
• W4383817004 cites W1977040399 @default.
• W4383817004 cites W1977702086 @default.
• W4383817004 cites W1997023449 @default.
• W4383817004 cites W1998302864 @default.
• W4383817004 cites W2000053769 @default.
• W4383817004 cites W2010094971 @default.
• W4383817004 cites W2015452134 @default.
• W4383817004 cites W2015727883 @default.
• W4383817004 cites W2031970939 @default.
• W4383817004 cites W2090458427 @default.
• W4383817004 cites W2104707233 @default.
• W4383817004 cites W2121212592 @default.
• W4383817004 cites W2154977172 @default.
• W4383817004 cites W2163925640 @default.
• W4383817004 cites W2223049285 @default.
• W4383817004 cites W2259483026 @default.
• W4383817004 cites W2412754298 @default.
• W4383817004 cites W2415033560 @default.
• W4383817004 cites W2462709496 @default.
• W4383817004 cites W2499659154 @default.
• W4383817004 cites W2768299799 @default.
• W4383817004 cites W2777542213 @default.
• W4383817004 cites W2792198246 @default.
• W4383817004 cites W2794330020 @default.
• W4383817004 cites W2805539079 @default.
• W4383817004 cites W2886242426 @default.
• W4383817004 cites W2888128356 @default.
• W4383817004 cites W2913863236 @default.
• W4383817004 cites W2944574015 @default.
• W4383817004 cites W2994702596 @default.
• W4383817004 cites W2997946065 @default.
• W4383817004 cites W3016461994 @default.
• W4383817004 cites W3019682838 @default.
• W4383817004 cites W3125529571 @default.
• W4383817004 cites W3133167713 @default.
• W4383817004 cites W3156161442 @default.
• W4383817004 cites W3192723735 @default.
• W4383817004 cites W3212336325 @default.
• W4383817004 cites W4200017596 @default.
• W4383817004 cites W4221094605 @default.
• W4383817004 cites W4233647994 @default.
• W4383817004 cites W4307951985 @default.
• W4383817004 cites W4309243956 @default.
• W4383817004 doi "https://doi.org/10.3389/fphar.2023.1145860" @default.
• W4383817004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37492091" @default.
• W4383817004 hasPublicationYear "2023" @default.
• W4383817004 type Work @default.
• W4383817004 citedByCount "0" @default.
• W4383817004 crossrefType "journal-article" @default.
• W4383817004 hasAuthorship W4383817004A5008687370 @default.
• W4383817004 hasAuthorship W4383817004A5010015599 @default.
• W4383817004 hasAuthorship W4383817004A5011376800 @default.
• W4383817004 hasAuthorship W4383817004A5019982577 @default.
• W4383817004 hasAuthorship W4383817004A5022015826 @default.
• W4383817004 hasAuthorship W4383817004A5023349336 @default.
• W4383817004 hasAuthorship W4383817004A5040711249 @default.
• W4383817004 hasAuthorship W4383817004A5041617090 @default.
• W4383817004 hasAuthorship W4383817004A5060879543 @default.
• W4383817004 hasAuthorship W4383817004A5068560871 @default.
• W4383817004 hasAuthorship W4383817004A5072937786 @default.
• W4383817004 hasAuthorship W4383817004A5091139356 @default.
• W4383817004 hasBestOaLocation W43838170041 @default.
• W4383817004 hasConcept C121608353 @default.
• W4383817004 hasConcept C126322002 @default.
• W4383817004 hasConcept C134018914 @default.
• W4383817004 hasConcept C171089720 @default.
• W4383817004 hasConcept C185592680 @default.
• W4383817004 hasConcept C2776235491 @default.
• W4383817004 hasConcept C2780994212 @default.
• W4383817004 hasConcept C519581460 @default.
• W4383817004 hasConcept C71924100 @default.
• W4383817004 hasConceptScore W4383817004C121608353 @default.
• W4383817004 hasConceptScore W4383817004C126322002 @default.
• W4383817004 hasConceptScore W4383817004C134018914 @default.
• W4383817004 hasConceptScore W4383817004C171089720 @default.
• W4383817004 hasConceptScore W4383817004C185592680 @default.
• W4383817004 hasConceptScore W4383817004C2776235491 @default.
• W4383817004 hasConceptScore W4383817004C2780994212 @default.
• W4383817004 hasConceptScore W4383817004C519581460 @default.
• W4383817004 hasConceptScore W4383817004C71924100 @default.
• W4383817004 hasFunder F4320320997 @default.

• next