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- W4383895246 abstract "The presence of Shiga toxin-producing Escherichia coli (STEC) in dairy products made with raw milk is a major concern for food safety authorities and industries. Two approaches have been proposed to isolate STEC from food. In the IC-Protocol (immuno-concentration protocol), specific serogroups are identified in the enrichment broth after detection of the stx and eae genes. An immuno-concentration of the targeted serogroups is performed before isolating them on specific media. In the DI-Protocol (direct isolation protocol), a direct isolation of all STEC present in the enrichment broth is carried out after detection of stx genes. We compared the ability of these 2 methods to isolate STEC O26:H11, O103:H2, O111:H8, O145:H28 and O157:H7 after artificial inoculation in 4 different raw milk cheeses. Across all serogroups and cheese types, STEC were isolated in 83.3% of samples when using the IC-Protocol but only 53.3% of samples with the DI-Protocol. For two cheese types, the DI-Protocol failed to isolate STEC O157:H7 strains altogether. Our results suggest that IC-Protocol is a robust methodology to effectively isolate STEC across a range of cheese types." @default.
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- W4383895246 date "2023-09-01" @default.
- W4383895246 modified "2023-09-26" @default.
- W4383895246 title "Comparison of Two Shiga Toxin-producing Escherichia coli (STEC) Isolation Protocols in Raw Cow’s Milk Cheese Enrichment Broths: Direct STEC Isolation Versus Techniques Based on Immuno-concentration" @default.
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- W4383895246 doi "https://doi.org/10.1016/j.jfp.2023.100128" @default.
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