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• W4383955222 endingPage "1819" @default.
• W4383955222 startingPage "1819" @default.
• W4383955222 abstract "The hypothesis about the role of the cortical cytoskeleton as the primary mechanosensor was tested. Drosophila melanogaster oocytes were exposed to simulated microgravity (by 3D clinorotation in random directions with 4 rotations per minute-sµg group) and hypergravity at the 2 g level (by centrifugal force from one axis rotation-hg group) for 30, 90, and 210 min without and with cytochalasin B, colchicine, acrylamide, and calyculin A. Cell stiffness was measured by atomic force microscopy, protein content in the membrane and cytoplasmic fractions by Western blotting, and cellular respiration by polarography. The obtained results indicate that the stiffness of the cortical cytoskeleton of Drosophila melanogaster oocytes decreases in simulated micro- (after 90 min) and hypergravity (after 30 min), possibly due to intermediate filaments. The cell stiffness recovered after 210 min in the hg group, but intact microtubules were required for this. Already after 30 min of exposure to sµg, the cross-sectional area of oocytes decreased, which indicates deformation, and the singed protein, which organizes microfilaments into longitudinal bundles, diffused from the cortical cytoskeleton into the cytoplasm. Under hg, after 30 min, the cross-sectional area of the oocytes increased, and the proteins that organize filament networks, alpha-actinin and spectrin, diffused from the cortical cytoskeleton." @default.
• W4383955222 created "2023-07-12" @default.
• W4383955222 creator A5025761016 @default.
• W4383955222 date "2023-07-10" @default.
• W4383955222 modified "2023-10-14" @default.
• W4383955222 title "The Mechanoreception in Drosophila melanogaster Oocyte under Modeling Micro- and Hypergravity" @default.
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• W4383955222 doi "https://doi.org/10.3390/cells12141819" @default.
• W4383955222 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37508484" @default.
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