FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384037328> ?p ?o ?g. }

• W4384037328 endingPage "1827" @default.
• W4384037328 startingPage "1827" @default.
• W4384037328 abstract "Objectives: The optimal healing of skin wounds, deep burns, and chronic ulcers is an important clinical problem. Attempts to solve it have been driving the search for skin equivalents based on synthetic or natural polymers. Methods: Consistent with this endeavor, we used regenerated silk fibroin (SF) from Bombyx mori to produce a novel compound scaffold by welding a 3D carded/hydroentangled SF-microfiber-based nonwoven layer (C/H-3D-SFnw; to support dermis engineering) to an electrospun 2D SF nanofiber layer (ESFN; a basal lamina surrogate). Next, we assessed-via scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy, differential scanning calorimetry, mono- and co-cultures of HaCaT keratinocytes and adult human dermal fibroblasts (HDFs), dsDNA assays, exosome isolation, double-antibody arrays, and angiogenesis assays-whether the C/H-3D-SFnws/ESFNs would allow the reconstitution of a functional human skin analog in vitro. Results: Physical analyses proved that the C/H-3D-SFnws/ESFNs met the requirements for human soft-tissue-like implants. dsDNA assays revealed that co-cultures of HaCaTs (on the 2D ESFN surface) and HDFs (inside the 3D C/H-3D-SFnws) grew more intensely than did the respective monocultures. Double-antibody arrays showed that the CD9+/CD81+ exosomes isolated from the 14-day pooled growth media of HDF and/or HaCaT mono- or co-cultures conveyed 35 distinct angiogenic/growth factors (AGFs). However, versus monocultures' exosomes, HaCaT/HDF co-cultures' exosomes (i) transported larger amounts of 15 AGFs, i.e., PIGF, ANGPT-1, bFGF, Tie-2, Angiogenin, VEGF-A, VEGF-D, TIMP-1/-2, GRO-α/-β/-γ, IL-1β, IL-6, IL-8, MMP-9, and MCP-1, and (ii) significantly more strongly stimulated human dermal microvascular endothelial cells to migrate and assemble tubes/nodes in vitro. Conclusions: Our results showed that both cell-cell and cell-SF interactions boosted the exosomal release of AGFs from HaCaTs/HDFs co-cultured on C/H-3D-SFnws/ESFNs. Hence, such exosomes are an asset for prospective clinical applications as they advance cell growth and neoangiogenesis and consequently graft take and skin healing. Moreover, this new integument analog could be instrumental in preclinical and translational studies on human skin pathophysiology and regeneration." @default.
• W4384037328 created "2023-07-13" @default.
• W4384037328 creator A5011627728 @default.
• W4384037328 creator A5024113446 @default.
• W4384037328 creator A5041652591 @default.
• W4384037328 creator A5052407008 @default.
• W4384037328 creator A5061275176 @default.
• W4384037328 date "2023-07-11" @default.
• W4384037328 modified "2023-10-18" @default.
• W4384037328 title "Human Keratinocytes and Fibroblasts Co-Cultured on Silk Fibroin Scaffolds Exosomally Overrelease Angiogenic and Growth Factors" @default.
• W4384037328 cites W124555615 @default.
• W4384037328 cites W1523145283 @default.
• W4384037328 cites W1783580437 @default.
• W4384037328 cites W1811212121 @default.
• W4384037328 cites W1934636952 @default.
• W4384037328 cites W1948065235 @default.
• W4384037328 cites W1960512949 @default.
• W4384037328 cites W1963598532 @default.
• W4384037328 cites W1970584042 @default.
• W4384037328 cites W1973866395 @default.
• W4384037328 cites W1976747819 @default.
• W4384037328 cites W1980357865 @default.
• W4384037328 cites W1982771591 @default.
• W4384037328 cites W1984933192 @default.
• W4384037328 cites W1984983173 @default.
• W4384037328 cites W1985306804 @default.
• W4384037328 cites W1996786865 @default.
• W4384037328 cites W1998499591 @default.
• W4384037328 cites W1999956482 @default.
• W4384037328 cites W2000299979 @default.
• W4384037328 cites W2000738714 @default.
• W4384037328 cites W2000946764 @default.
• W4384037328 cites W2002286451 @default.
• W4384037328 cites W2003676894 @default.
• W4384037328 cites W2003788548 @default.
• W4384037328 cites W2006779391 @default.
• W4384037328 cites W2010087085 @default.
• W4384037328 cites W2010313128 @default.
• W4384037328 cites W2015213060 @default.
• W4384037328 cites W2016806826 @default.
• W4384037328 cites W2022356464 @default.
• W4384037328 cites W2023820705 @default.
• W4384037328 cites W2024788464 @default.
• W4384037328 cites W2026393226 @default.
• W4384037328 cites W2039015141 @default.
• W4384037328 cites W2040279203 @default.
• W4384037328 cites W2041309288 @default.
• W4384037328 cites W2041643931 @default.
• W4384037328 cites W2042207484 @default.
• W4384037328 cites W2046334531 @default.
• W4384037328 cites W2047199455 @default.
• W4384037328 cites W2047719515 @default.
• W4384037328 cites W2049291124 @default.
• W4384037328 cites W2052116137 @default.
• W4384037328 cites W2055424631 @default.
• W4384037328 cites W2056105344 @default.
• W4384037328 cites W2057356848 @default.
• W4384037328 cites W2057500843 @default.
• W4384037328 cites W2060077653 @default.
• W4384037328 cites W2065105677 @default.
• W4384037328 cites W2076415360 @default.
• W4384037328 cites W2078958131 @default.
• W4384037328 cites W2079962061 @default.
• W4384037328 cites W2080137491 @default.
• W4384037328 cites W2085804760 @default.
• W4384037328 cites W2087993164 @default.
• W4384037328 cites W2089066799 @default.
• W4384037328 cites W2093160601 @default.
• W4384037328 cites W2094365218 @default.
• W4384037328 cites W2097881605 @default.
• W4384037328 cites W2099491642 @default.
• W4384037328 cites W2102687166 @default.
• W4384037328 cites W2103984819 @default.
• W4384037328 cites W2107492558 @default.
• W4384037328 cites W2108375854 @default.
• W4384037328 cites W2109041605 @default.
• W4384037328 cites W2122741606 @default.
• W4384037328 cites W2125050685 @default.
• W4384037328 cites W2126864422 @default.
• W4384037328 cites W2130892977 @default.
• W4384037328 cites W2133353385 @default.
• W4384037328 cites W2143372911 @default.
• W4384037328 cites W2143464311 @default.
• W4384037328 cites W2143729276 @default.
• W4384037328 cites W2151752998 @default.
• W4384037328 cites W2157908104 @default.
• W4384037328 cites W2159193579 @default.
• W4384037328 cites W2161922223 @default.
• W4384037328 cites W2167839699 @default.
• W4384037328 cites W2169336903 @default.
• W4384037328 cites W2169557810 @default.
• W4384037328 cites W2169822262 @default.
• W4384037328 cites W2171672500 @default.
• W4384037328 cites W2186509953 @default.
• W4384037328 cites W2187447045 @default.
• W4384037328 cites W2220539909 @default.
• W4384037328 cites W2274295713 @default.
• W4384037328 cites W2292056912 @default.

• next