Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384157219> ?p ?o ?g. }
- W4384157219 endingPage "110289" @default.
- W4384157219 startingPage "110289" @default.
- W4384157219 abstract "β-Glucosidases with high catalytic activity and glucose tolerant properties possess promising applications in lignocellulose-based industries. To obtain enzymes possessing these properties, a semi-rational strategy was employed to engineer the glucose-stimulating β-glucosidase Bgl2A for high cellobiose hydrolysis activity. A total of 18 mutants were constructed. A22S, V224D, and A22S/V224D exhibited high specific activities of 272.06, 237.60, and 239.29 U/mg toward cellobiose, which were 2.5- to 2.8-fold of Bgl2A. A22S, V224D, and A22S/V224D exhibited increased kcat values, which were 2.7- to 3.1-fold of Bgl2A. A22S and V224D maintained glucose-stimulating property, whereas A22S/V224D lost it. Using 150 g/L cellobiose as the substrate, the amount of glucose produced by A22S was the highest, yielding 129.70 g/L glucose after 3 h reaction at 35 °C. The synergistic effects of the engineered enzymes with commercial cellulase on hydrolyzing cellulose were investigated. Supplemented with the commercial cellulase and A22S, the highest glucose amount of 23.30 g/L was yielded from cellulose with hydrolysis rate of 21.02 %. Given its high cellobiose hydrolysis activity and glucose-stimulating properties, A22S can be used as a component of enzyme cocktail to match mesophilic cellulases for efficient cellulose hydrolysis." @default.
- W4384157219 created "2023-07-14" @default.
- W4384157219 creator A5031366477 @default.
- W4384157219 creator A5036521207 @default.
- W4384157219 creator A5040806189 @default.
- W4384157219 creator A5052844740 @default.
- W4384157219 creator A5074140059 @default.
- W4384157219 creator A5080103954 @default.
- W4384157219 creator A5085321310 @default.
- W4384157219 date "2023-09-01" @default.
- W4384157219 modified "2023-10-18" @default.
- W4384157219 title "Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A" @default.
- W4384157219 cites W1851764808 @default.
- W4384157219 cites W1934375444 @default.
- W4384157219 cites W1967293793 @default.
- W4384157219 cites W1970325009 @default.
- W4384157219 cites W1972926987 @default.
- W4384157219 cites W2005133608 @default.
- W4384157219 cites W2014958041 @default.
- W4384157219 cites W2022005829 @default.
- W4384157219 cites W2034323925 @default.
- W4384157219 cites W2034451231 @default.
- W4384157219 cites W2049539935 @default.
- W4384157219 cites W2050543826 @default.
- W4384157219 cites W2065955252 @default.
- W4384157219 cites W2082475640 @default.
- W4384157219 cites W2083381259 @default.
- W4384157219 cites W2084681492 @default.
- W4384157219 cites W2086538274 @default.
- W4384157219 cites W2089643533 @default.
- W4384157219 cites W2105015569 @default.
- W4384157219 cites W2107769173 @default.
- W4384157219 cites W2115436659 @default.
- W4384157219 cites W2124231317 @default.
- W4384157219 cites W2130227531 @default.
- W4384157219 cites W2136038574 @default.
- W4384157219 cites W2158673136 @default.
- W4384157219 cites W2171509237 @default.
- W4384157219 cites W2184817372 @default.
- W4384157219 cites W2212126140 @default.
- W4384157219 cites W2228653419 @default.
- W4384157219 cites W2318958768 @default.
- W4384157219 cites W2339015210 @default.
- W4384157219 cites W2397885266 @default.
- W4384157219 cites W2533944651 @default.
- W4384157219 cites W2550551973 @default.
- W4384157219 cites W2596564805 @default.
- W4384157219 cites W2767442151 @default.
- W4384157219 cites W2806451027 @default.
- W4384157219 cites W2899397040 @default.
- W4384157219 cites W2903074283 @default.
- W4384157219 cites W2916436781 @default.
- W4384157219 cites W3008149296 @default.
- W4384157219 cites W3016097852 @default.
- W4384157219 cites W3016277656 @default.
- W4384157219 cites W3047506258 @default.
- W4384157219 cites W3071774811 @default.
- W4384157219 cites W309017724 @default.
- W4384157219 cites W3115050483 @default.
- W4384157219 cites W3209598526 @default.
- W4384157219 cites W4211245981 @default.
- W4384157219 cites W4230416437 @default.
- W4384157219 doi "https://doi.org/10.1016/j.enzmictec.2023.110289" @default.
- W4384157219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37473697" @default.
- W4384157219 hasPublicationYear "2023" @default.
- W4384157219 type Work @default.
- W4384157219 citedByCount "1" @default.
- W4384157219 countsByYear W43841572192023 @default.
- W4384157219 crossrefType "journal-article" @default.
- W4384157219 hasAuthorship W4384157219A5031366477 @default.
- W4384157219 hasAuthorship W4384157219A5036521207 @default.
- W4384157219 hasAuthorship W4384157219A5040806189 @default.
- W4384157219 hasAuthorship W4384157219A5052844740 @default.
- W4384157219 hasAuthorship W4384157219A5074140059 @default.
- W4384157219 hasAuthorship W4384157219A5080103954 @default.
- W4384157219 hasAuthorship W4384157219A5085321310 @default.
- W4384157219 hasBestOaLocation W43841572191 @default.
- W4384157219 hasConcept C100544194 @default.
- W4384157219 hasConcept C141603559 @default.
- W4384157219 hasConcept C181199279 @default.
- W4384157219 hasConcept C185592680 @default.
- W4384157219 hasConcept C18903297 @default.
- W4384157219 hasConcept C2777289219 @default.
- W4384157219 hasConcept C2779251873 @default.
- W4384157219 hasConcept C2780074830 @default.
- W4384157219 hasConcept C2780412358 @default.
- W4384157219 hasConcept C2780777981 @default.
- W4384157219 hasConcept C41183919 @default.
- W4384157219 hasConcept C523546767 @default.
- W4384157219 hasConcept C53270655 @default.
- W4384157219 hasConcept C54355233 @default.
- W4384157219 hasConcept C55493867 @default.
- W4384157219 hasConcept C56856141 @default.
- W4384157219 hasConcept C63223902 @default.
- W4384157219 hasConcept C86803240 @default.
- W4384157219 hasConcept C94412978 @default.
- W4384157219 hasConceptScore W4384157219C100544194 @default.
- W4384157219 hasConceptScore W4384157219C141603559 @default.