FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384199313> ?p ?o ?g. }

• W4384199313 abstract "Neurotrophin-4 (NT-4), a neurotrophic factor, appears to affect early embryonic development because it is secreted not only by neurons but also by oviductal and uterine epithelial cells. However, no studies have characterized the effects of NT-4 on early embryonic development in pigs. In this study, we applied the experimental model of parthenogenetic-activation (PA)-derived embryos. Herein, we investigated the effect of NT-4 supplementation during the in vitro culture (IVC) of embryos, analyzed the transcription levels of specific genes, and outlined the first cell lineage specification for porcine PA-derived blastocysts. We confirmed that NT-4 and its receptor proteins were localized in both the inner cell mass (ICM) and trophectoderm (TE) in porcine blastocysts. Across different concentrations (0, 1, 10, and 100 ng/mL) of NT-4 supplementation, the optimal concentration of NT-4 to improve the developmental competence of porcine parthenotes was 10 ng/mL. NT-4 supplementation during porcine IVC significantly ( p &lt; 0.05) increased the proportion of TE cells by inducing the transcription of TE lineage markers ( CDX2 , PPAG3 , and GATA3 transcripts). NT-4 also reduced blastocyst apoptosis by regulating the transcription of apoptosis-related genes ( BAX and BCL2L1 transcripts) and improved blastocyst quality via the interaction of neurotrophin-, Hippo-yes-associated protein (Hippo-YAP) and mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway. Additionally, NT-4 supplementation during IVC significantly ( p &lt; 0.05) increased YAP1 transcript levels and significantly ( p &lt; 0.01) decreased LATS2 transcript levels, respectively, in the porcine PA-derived blastocysts. We also confirmed through fluorescence intensity that the YAP1 protein was significantly ( p &lt; 0.001) increased in the NT-4-treated blastocysts compared with that in the control. NT-4 also promoted differentiation into the TE lineage rather than into the ICM lineage during porcine early embryonic development. In conclusion, 10 ng/mL NT-4 supplementation enhanced blastocyst quality by regulating the apoptosis- and TE lineage specification-related genes and interacting with neurotrophin-, Hippo-YAP-, and MAPK/ERK signaling pathway during porcine in vitro embryo development." @default.
• W4384199313 created "2023-07-14" @default.
• W4384199313 creator A5003200124 @default.
• W4384199313 creator A5017436471 @default.
• W4384199313 creator A5039285626 @default.
• W4384199313 creator A5048431969 @default.
• W4384199313 creator A5057137644 @default.
• W4384199313 creator A5086987446 @default.
• W4384199313 creator A5091264637 @default.
• W4384199313 date "2023-07-13" @default.
• W4384199313 modified "2023-10-16" @default.
• W4384199313 title "Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development" @default.
• W4384199313 cites W1558589684 @default.
• W4384199313 cites W1569950491 @default.
• W4384199313 cites W1886654397 @default.
• W4384199313 cites W1956026056 @default.
• W4384199313 cites W1965820183 @default.
• W4384199313 cites W1966576194 @default.
• W4384199313 cites W1971719282 @default.
• W4384199313 cites W1973994766 @default.
• W4384199313 cites W1979298412 @default.
• W4384199313 cites W1979499962 @default.
• W4384199313 cites W1980113816 @default.
• W4384199313 cites W1981088384 @default.
• W4384199313 cites W1986944049 @default.
• W4384199313 cites W1988658711 @default.
• W4384199313 cites W1989053030 @default.
• W4384199313 cites W1992135018 @default.
• W4384199313 cites W1994144105 @default.
• W4384199313 cites W2000105010 @default.
• W4384199313 cites W2000469450 @default.
• W4384199313 cites W2000701513 @default.
• W4384199313 cites W2004848920 @default.
• W4384199313 cites W2006499356 @default.
• W4384199313 cites W2007634751 @default.
• W4384199313 cites W2008288412 @default.
• W4384199313 cites W2008793945 @default.
• W4384199313 cites W2009473720 @default.
• W4384199313 cites W2012305943 @default.
• W4384199313 cites W2013045396 @default.
• W4384199313 cites W2034328033 @default.
• W4384199313 cites W2037588567 @default.
• W4384199313 cites W2040216616 @default.
• W4384199313 cites W2044461908 @default.
• W4384199313 cites W2047623407 @default.
• W4384199313 cites W2053262948 @default.
• W4384199313 cites W2058335423 @default.
• W4384199313 cites W2072590237 @default.
• W4384199313 cites W2078035928 @default.
• W4384199313 cites W2078154411 @default.
• W4384199313 cites W2079658565 @default.
• W4384199313 cites W2080480991 @default.
• W4384199313 cites W2102872405 @default.
• W4384199313 cites W2105142263 @default.
• W4384199313 cites W2121670946 @default.
• W4384199313 cites W2121817763 @default.
• W4384199313 cites W2124366353 @default.
• W4384199313 cites W2128718933 @default.
• W4384199313 cites W2128960139 @default.
• W4384199313 cites W2130104786 @default.
• W4384199313 cites W2131074211 @default.
• W4384199313 cites W2140836258 @default.
• W4384199313 cites W2153068303 @default.
• W4384199313 cites W2167978765 @default.
• W4384199313 cites W2308326102 @default.
• W4384199313 cites W2466636855 @default.
• W4384199313 cites W2470223656 @default.
• W4384199313 cites W2561447904 @default.
• W4384199313 cites W2563721483 @default.
• W4384199313 cites W2585872907 @default.
• W4384199313 cites W2589272435 @default.
• W4384199313 cites W2607466014 @default.
• W4384199313 cites W2730940069 @default.
• W4384199313 cites W2896586298 @default.
• W4384199313 cites W2900496978 @default.
• W4384199313 cites W2913337859 @default.
• W4384199313 cites W2938968537 @default.
• W4384199313 cites W2965710886 @default.
• W4384199313 cites W2995464460 @default.
• W4384199313 cites W3004138966 @default.
• W4384199313 cites W3009796961 @default.
• W4384199313 cites W3011815506 @default.
• W4384199313 cites W3111720993 @default.
• W4384199313 cites W3136478187 @default.
• W4384199313 cites W3181996715 @default.
• W4384199313 cites W3190000207 @default.
• W4384199313 cites W3193695519 @default.
• W4384199313 cites W3198567767 @default.
• W4384199313 cites W3206741012 @default.
• W4384199313 cites W3213424320 @default.
• W4384199313 cites W3216584085 @default.
• W4384199313 cites W4211063797 @default.
• W4384199313 cites W4284891565 @default.
• W4384199313 cites W4292686471 @default.
• W4384199313 cites W4378746514 @default.
• W4384199313 cites W48782725 @default.
• W4384199313 cites W52878410 @default.
• W4384199313 doi "https://doi.org/10.3389/fcell.2023.1194596" @default.
• W4384199313 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37519302" @default.
• W4384199313 hasPublicationYear "2023" @default.

• next