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- W4384205525 abstract "Abstract Formate dehydrogenase (FDH) is a D -2-hydroxy acid dehydrogenase, which can reversibly reduce CO 2 to formate and thus act as non-photosynthetic CO 2 reductase. In order to increase catalytic efficiency of formate dehydrogenase for CO 2 reduction, the formate dehydrogenase Cb FDH M2 was used as the parent to conduct single-site saturation and iterative combinatorial mutagenesis, and two mutants V328I/F285W and V354G/F285W were obtained of which reduction activity was about two times more than the Cb FDH M2 and had greater potential in CO 2 reduction. The optimal temperature for V328I/F285W and V354G/F285W was 55℃, and the optimal pH was 9.0. Then the molecular basis for its improvement of biochemical characteristics were preliminarily elucidated by computer-aided methods. All of these results further established a solid foundation for molecular modification of formate dehydrogenase and CO 2 reduction." @default.
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- W4384205525 date "2023-07-13" @default.
- W4384205525 modified "2023-10-18" @default.
- W4384205525 title "Engineering of formate dehydrogenase for improving conversion potential of carbon dioxide to formate" @default.
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