Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384211097> ?p ?o ?g. }
- W4384211097 endingPage "213" @default.
- W4384211097 startingPage "173" @default.
- W4384211097 abstract "Avian (ortho)reovirus (ARV), which belongs to Reoviridae family, is a major domestic fowl pathogen and is the causative agent of viral tenosynovitis and chronic respiratory disease in chicken. ARV replicates within cytoplasmic inclusions, so-called viral factories, that form by phase separation and thus belong to a wider class of biological condensates. Here, we evaluate different optical imaging methods that have been developed or adapted to follow formation, fluidity and composition of viral factories and compare them with the complementary structural information obtained by well-established transmission electron microscopy and electron tomography. The molecular and cellular biology aspects for setting up and following virus infection in cells by imaging are described first. We then demonstrate that a wide-field version of fluorescence recovery after photobleaching is an effective tool to measure fluidity of mobile viral factories. A new technique, holotomographic phase microscopy, is then used for imaging of viral factory formation in live cells in three dimensions. Confocal Raman microscopy of infected cells provides “chemical” contrast for label-free segmentation of images and addresses important questions about biomolecular concentrations within viral factories and other biological condensates. Optical imaging is complemented by electron microscopy and tomography which supply higher resolution structural detail, including visualization of individual virions within the three-dimensional cellular context." @default.
- W4384211097 created "2023-07-14" @default.
- W4384211097 creator A5001569722 @default.
- W4384211097 creator A5022335632 @default.
- W4384211097 creator A5061535309 @default.
- W4384211097 creator A5061858006 @default.
- W4384211097 creator A5062536182 @default.
- W4384211097 creator A5064276934 @default.
- W4384211097 creator A5080403737 @default.
- W4384211097 date "2023-01-01" @default.
- W4384211097 modified "2023-10-01" @default.
- W4384211097 title "Shedding light on reovirus assembly—Multimodal imaging of viral factories" @default.
- W4384211097 cites W1519297291 @default.
- W4384211097 cites W1524626666 @default.
- W4384211097 cites W1566889438 @default.
- W4384211097 cites W1581201881 @default.
- W4384211097 cites W1749812141 @default.
- W4384211097 cites W1859483888 @default.
- W4384211097 cites W1920657318 @default.
- W4384211097 cites W1946312082 @default.
- W4384211097 cites W1962467645 @default.
- W4384211097 cites W1973343208 @default.
- W4384211097 cites W1989788002 @default.
- W4384211097 cites W1996296369 @default.
- W4384211097 cites W1996550262 @default.
- W4384211097 cites W2000481871 @default.
- W4384211097 cites W2005922038 @default.
- W4384211097 cites W2007316692 @default.
- W4384211097 cites W2007879544 @default.
- W4384211097 cites W2017934591 @default.
- W4384211097 cites W2019564765 @default.
- W4384211097 cites W2022846740 @default.
- W4384211097 cites W2024437068 @default.
- W4384211097 cites W2039702920 @default.
- W4384211097 cites W2057219336 @default.
- W4384211097 cites W2061148101 @default.
- W4384211097 cites W2066824126 @default.
- W4384211097 cites W2067386938 @default.
- W4384211097 cites W2071002552 @default.
- W4384211097 cites W2072274257 @default.
- W4384211097 cites W2075071462 @default.
- W4384211097 cites W2085662236 @default.
- W4384211097 cites W2087290848 @default.
- W4384211097 cites W2088376671 @default.
- W4384211097 cites W2096775204 @default.
- W4384211097 cites W2130384960 @default.
- W4384211097 cites W2134082339 @default.
- W4384211097 cites W2139066576 @default.
- W4384211097 cites W2142161929 @default.
- W4384211097 cites W2156287975 @default.
- W4384211097 cites W2171566201 @default.
- W4384211097 cites W2487704570 @default.
- W4384211097 cites W2580035316 @default.
- W4384211097 cites W2611326286 @default.
- W4384211097 cites W2618036898 @default.
- W4384211097 cites W2619230229 @default.
- W4384211097 cites W2713865210 @default.
- W4384211097 cites W2734356283 @default.
- W4384211097 cites W2774148651 @default.
- W4384211097 cites W2886971359 @default.
- W4384211097 cites W2888622657 @default.
- W4384211097 cites W2901832822 @default.
- W4384211097 cites W2904558843 @default.
- W4384211097 cites W2910392468 @default.
- W4384211097 cites W2924806352 @default.
- W4384211097 cites W2943919947 @default.
- W4384211097 cites W2950424426 @default.
- W4384211097 cites W2969936848 @default.
- W4384211097 cites W2970894940 @default.
- W4384211097 cites W2994730959 @default.
- W4384211097 cites W3038056843 @default.
- W4384211097 cites W3087654678 @default.
- W4384211097 cites W3090871378 @default.
- W4384211097 cites W3113117617 @default.
- W4384211097 cites W3118330800 @default.
- W4384211097 cites W3174900324 @default.
- W4384211097 cites W3179164422 @default.
- W4384211097 cites W3181352425 @default.
- W4384211097 cites W3200801568 @default.
- W4384211097 cites W3201894557 @default.
- W4384211097 cites W3203328811 @default.
- W4384211097 cites W3210719946 @default.
- W4384211097 cites W4241300192 @default.
- W4384211097 cites W4295441599 @default.
- W4384211097 cites W4295832271 @default.
- W4384211097 cites W4306790272 @default.
- W4384211097 cites W4318068983 @default.
- W4384211097 doi "https://doi.org/10.1016/bs.aivir.2023.06.002" @default.
- W4384211097 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37524481" @default.
- W4384211097 hasPublicationYear "2023" @default.
- W4384211097 type Work @default.
- W4384211097 citedByCount "0" @default.
- W4384211097 crossrefType "book-chapter" @default.
- W4384211097 hasAuthorship W4384211097A5001569722 @default.
- W4384211097 hasAuthorship W4384211097A5022335632 @default.
- W4384211097 hasAuthorship W4384211097A5061535309 @default.
- W4384211097 hasAuthorship W4384211097A5061858006 @default.
- W4384211097 hasAuthorship W4384211097A5062536182 @default.