Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384338005> ?p ?o ?g. }
- W4384338005 abstract "Pancreatic cancer is a leading cause of cancer death due to its early metastasis and limited response to the current therapies. Metastasis is a complicated multistep process, which is determined by complex genetic alterations. Despite the identification of many metastasis-related genes, distinguishing the drivers from numerous passengers and establishing the causality in cancer pathophysiology remains challenging. Here, we established a high-throughput and piggyBac transposon-based genetic screening platform, which enables either reduced or increased expression of chromosomal genes near the incorporation site of the gene search vector cassette that contains a doxycycline-regulated promoter. Using this strategy, we identified YWHAZ as a key regulator of pancreatic cancer metastasis. We demonstrated that functional activation of Ywhaz by the gene search vector led to enhanced metastatic capability in mouse pancreatic cancer cells. The metastasis-promoting role of YWHAZ was further validated in human pancreatic cancer cells. Overexpression of YWHAZ resulted in more aggressive metastatic phenotypes in vitro and a shorter survival rate in vivo by modulating epithelial-to-mesenchymal transition. Hence, our study established a high-throughput screening method to investigate the functional relevance of novel genes and validated YWHAZ as a key regulator of pancreatic cancer metastasis." @default.
- W4384338005 created "2023-07-15" @default.
- W4384338005 creator A5015120996 @default.
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- W4384338005 creator A5089883344 @default.
- W4384338005 date "2023-07-14" @default.
- W4384338005 modified "2023-10-10" @default.
- W4384338005 title "High-throughput functional screen identifies YWHAZ as a key regulator of pancreatic cancer metastasis" @default.
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