Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384341007> ?p ?o ?g. }
- W4384341007 endingPage "1839" @default.
- W4384341007 startingPage "1839" @default.
- W4384341007 abstract "Human Fe(II)/α-ketoglutarate-dependent dioxygenase ABH2 plays a crucial role in the direct reversal repair of nonbulky alkyl lesions in DNA nucleobases, e.g., N1-methyladenine (m1A), N3-methylcytosine (m3C), and some etheno derivatives. Moreover, ABH2 is capable of a less efficient oxidation of an epigenetic DNA mark called 5-methylcytosine (m5C), which typically is a specific target of DNA dioxygenases from the TET family. In this study, to elucidate the mechanism of the substrate specificity of ABH2, we investigated the role of several active-site amino acid residues. Functional mapping of the lesion-binding pocket was performed through the analysis of the functions of Tyr122, Ile168, and Asp173 in the damaged base recognition mechanism. Interactions of wild-type ABH2, or its mutants Y122A, I168A, or D173A, with damaged DNA containing the methylated base m1A or m3C or the epigenetic marker m5C were analyzed by molecular dynamics simulations and kinetic assays. Comparative analysis of the enzymes revealed an effect of the substitutions on DNA binding and on catalytic activity. Obtained data clearly demonstrate the effect of the tested amino acid residues on the catalytic activity of the enzymes rather than the DNA-binding ability. Taken together, these data shed light on the molecular and kinetic consequences of the substitution of active-site residues for the mechanism of the substrate recognition." @default.
- W4384341007 created "2023-07-15" @default.
- W4384341007 creator A5003204998 @default.
- W4384341007 creator A5027490104 @default.
- W4384341007 creator A5027671045 @default.
- W4384341007 creator A5040453516 @default.
- W4384341007 creator A5057821249 @default.
- W4384341007 creator A5064506934 @default.
- W4384341007 creator A5065659221 @default.
- W4384341007 date "2023-07-13" @default.
- W4384341007 modified "2023-09-25" @default.
- W4384341007 title "Individual Contributions of Amido Acid Residues Tyr122, Ile168, and Asp173 to the Activity and Substrate Specificity of Human DNA Dioxygenase ABH2" @default.
- W4384341007 cites W1031578623 @default.
- W4384341007 cites W1510319360 @default.
- W4384341007 cites W1883201575 @default.
- W4384341007 cites W1968758965 @default.
- W4384341007 cites W1969836007 @default.
- W4384341007 cites W1972021276 @default.
- W4384341007 cites W1976161355 @default.
- W4384341007 cites W1976499671 @default.
- W4384341007 cites W1981225934 @default.
- W4384341007 cites W1983255679 @default.
- W4384341007 cites W1983338915 @default.
- W4384341007 cites W1984260352 @default.
- W4384341007 cites W1984516599 @default.
- W4384341007 cites W1986849535 @default.
- W4384341007 cites W1997453048 @default.
- W4384341007 cites W2003019599 @default.
- W4384341007 cites W2013873134 @default.
- W4384341007 cites W2015688756 @default.
- W4384341007 cites W2017991488 @default.
- W4384341007 cites W2019065642 @default.
- W4384341007 cites W2021039243 @default.
- W4384341007 cites W2035687084 @default.
- W4384341007 cites W2042765650 @default.
- W4384341007 cites W2045683699 @default.
- W4384341007 cites W2051498033 @default.
- W4384341007 cites W2057477511 @default.
- W4384341007 cites W2057701529 @default.
- W4384341007 cites W2059566793 @default.
- W4384341007 cites W2068198916 @default.
- W4384341007 cites W2068605146 @default.
- W4384341007 cites W2069421427 @default.
- W4384341007 cites W2069705658 @default.
- W4384341007 cites W2073327098 @default.
- W4384341007 cites W2081046982 @default.
- W4384341007 cites W2081693079 @default.
- W4384341007 cites W2084928359 @default.
- W4384341007 cites W2087171433 @default.
- W4384341007 cites W2088863316 @default.
- W4384341007 cites W2100673592 @default.
- W4384341007 cites W2103377484 @default.
- W4384341007 cites W2107770760 @default.
- W4384341007 cites W2110102140 @default.
- W4384341007 cites W2114299644 @default.
- W4384341007 cites W2122199548 @default.
- W4384341007 cites W2126840450 @default.
- W4384341007 cites W2128572087 @default.
- W4384341007 cites W2136270995 @default.
- W4384341007 cites W2142427369 @default.
- W4384341007 cites W2147993766 @default.
- W4384341007 cites W2167029191 @default.
- W4384341007 cites W2168240189 @default.
- W4384341007 cites W2176312424 @default.
- W4384341007 cites W2220012877 @default.
- W4384341007 cites W2326169261 @default.
- W4384341007 cites W2335712643 @default.
- W4384341007 cites W2344252179 @default.
- W4384341007 cites W2404280981 @default.
- W4384341007 cites W2405845840 @default.
- W4384341007 cites W2536595623 @default.
- W4384341007 cites W2748537268 @default.
- W4384341007 cites W2897150104 @default.
- W4384341007 cites W2944950731 @default.
- W4384341007 cites W2947295231 @default.
- W4384341007 cites W2952670656 @default.
- W4384341007 cites W3023972703 @default.
- W4384341007 cites W3082841381 @default.
- W4384341007 cites W3106706416 @default.
- W4384341007 cites W3134483462 @default.
- W4384341007 cites W3193617551 @default.
- W4384341007 cites W3203369321 @default.
- W4384341007 cites W4288045478 @default.
- W4384341007 cites W4289939892 @default.
- W4384341007 doi "https://doi.org/10.3390/cells12141839" @default.
- W4384341007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37508504" @default.
- W4384341007 hasPublicationYear "2023" @default.
- W4384341007 type Work @default.
- W4384341007 citedByCount "0" @default.
- W4384341007 crossrefType "journal-article" @default.
- W4384341007 hasAuthorship W4384341007A5003204998 @default.
- W4384341007 hasAuthorship W4384341007A5027490104 @default.
- W4384341007 hasAuthorship W4384341007A5027671045 @default.
- W4384341007 hasAuthorship W4384341007A5040453516 @default.
- W4384341007 hasAuthorship W4384341007A5057821249 @default.
- W4384341007 hasAuthorship W4384341007A5064506934 @default.
- W4384341007 hasAuthorship W4384341007A5065659221 @default.
- W4384341007 hasBestOaLocation W43843410071 @default.