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• W4384342746 abstract "Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis. In this study, we implemented, evaluated and validated according to the ISO 15,189 requirements, a multiplex real-time PCR assay to detect and identify the five human malaria parasites. DNA samples were extracted from whole blood or dried blood spots drawn from patients. Based on the External Quality Assessment (whole blood), this method shows 100% sensitivity and specificity. This PCR detected P. vivax up to 0.25 p/µl, P. falciparum and P. knowlesi up to 0.5 p/µl, P. ovale up to 1 p/µl and P. malariae up to 5 p/µl of blood. From blood spots (extraction from four punches), it detected P. vivax at 5 p/µl, P. falciparum, P. ovale and P. knowlesi at 20 p/µl and P. malariae at 125 p/µl. In conclusion, this quantitative PCR shows excellent performance, is easy to use and DNA saver. It is especially useful to actively screen large population groups and identify the five human malaria parasites in a context of low malaria transmission." @default.
• W4384342746 created "2023-07-15" @default.
• W4384342746 creator A5011841078 @default.
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• W4384342746 date "2023-07-14" @default.
• W4384342746 modified "2023-10-01" @default.
• W4384342746 title "Molecular detection of human Plasmodium species using a multiplex real time PCR" @default.
• W4384342746 cites W1966459680 @default.
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• W4384342746 doi "https://doi.org/10.1038/s41598-023-38621-9" @default.
• W4384342746 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37452123" @default.
• W4384342746 hasPublicationYear "2023" @default.
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