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• W4384404807 abstract "Abstract Bromus sterilis has evolved into a more predominant weed in the Czech Republic’s winter wheat fields, owing largely to the widespread application of pyroxsulam for its management. In this study, we report a biotype that has developed resistance to pyroxsulam and has also shown cross- resistance to other herbicides. Although no differences in ploidy levels or no mutations of acetolactate synthase (ALS) were detected, a significant elevation of ALS enzyme activity was observed in the R biotype. Through combined analysis of enzyme inhibition and total transcript expression (RNA-Seq), we have identified differentially expressed transcripts that potentially contribute to pyroxsulam metabolism. Furthermore, we observed a significant increase in the expression of genes involved in redox mechanisms and transporters that could contribute to enhanced resistance to pyroxulam in the R biotype. Our results present a novel understanding of herbicide resistance in B. sterilis through three distinct resistance mechanisms ( ALS gene overexpression, enhanced metabolism and reduced translocation) without mutation in the herbicide target protein. This understanding is the foundation for improving management strategies for herbicide resistant B. sterilis ." @default.
• W4384404807 created "2023-07-16" @default.
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• W4384404807 date "2023-07-15" @default.
• W4384404807 modified "2023-10-14" @default.
• W4384404807 title "Transcriptomic response in pyroxsulam-resistant and susceptible<i>Bromus sterilis</i>identified three distinct mechanisms of resistance" @default.
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• W4384404807 doi "https://doi.org/10.1101/2023.07.14.548957" @default.
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