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- W4384404823 abstract "Impaired function of the retinal pigment epithelium (RPE) resembles a hallmark in many retinal diseases; thus, co-cultivation models with RPE and retinal explants are useful to investigate these. Here, we present an easy-to-handle direct co-cultivation protocol, containing a functional, primary RPE monolayer and retinal explants. We describe in detail steps for establishing the monolayer and the direct co-cultivation. Techniques, which allow users to investigate the integrity and functionality of the RPE monolayer, are presented and the co-cultivation was tested under stress conditions." @default.
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- W4384404823 date "2023-09-01" @default.
- W4384404823 modified "2023-10-03" @default.
- W4384404823 title "Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures" @default.
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- W4384404823 doi "https://doi.org/10.1016/j.xpro.2023.102443" @default.
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