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- W4384408031 abstract "Rhizobia are soil bacteria that form nodules on legumes and fix atmospheric nitrogen, reducing the need for chemical fertilisers. In most studies rhizobia are described as alpha-proteobacteria and have been studied extensively for several decades. However, in recent years an increasing number of studies present beta- and gamma-proteobacteria strains as potential rhizobia-like bacteria. In this study we identify bacteria isolated from nodules of various legumes grown in Latvia, using a full-length 16S rRNA gene sequence, used for more precision, compared with a partial sequence of the gene. Bacterial strains in this study have been isolated from 1962–2019, part of which are rhizobia strains from a unique historical collection. This is the first time bacterial strains isolated from nodules in Latvian soils have been identified with molecular biology methods. A wide taxonomic diversity was detected – bacteria species from α-, β-, γ-proteobacteria classes and Paenibacillus polymyxa strains from the Bacilli class. P. polymyxa strains were detected only in the historical collection, while β- and γ-proteobacteria strains were obtained only from the newly isolated specimens, uncovering new potential strains for a commercial legume seed inoculum. Soil samples were collected, and phosphorus levels were determined. Several strains indicate phosphate solubilising properties." @default.
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- W4384408031 date "2023-07-15" @default.
- W4384408031 modified "2023-09-27" @default.
- W4384408031 title "Alpha-, gamma- and beta-proteobacteria detected in legume nodules in Latvia, using full-length 16S rRNA gene sequencing" @default.
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- W4384408031 doi "https://doi.org/10.1080/09064710.2023.2232681" @default.
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