FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384502057> ?p ?o ?g. }

• W4384502057 endingPage "89" @default.
• W4384502057 startingPage "80" @default.
• W4384502057 abstract "Prodrug design is a promising approach for reducing the off-target effects of therapeutic antibodies, particularly bispecific antibodies (bsAbs) that recruit T cells for activation; this design uses masking sequences that inhibit antibody binding until they reach the tumor microenvironment, where they are removed. In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the epitope sequence, and the extent of inhibition was proportional to the length of the PAS sequence. For masked bsAbs, T cell-binding ability, cancer growth inhibition effects, and T cell activation effects were also reduced depending on the length of PAS and were fully restored upon removing PAS sequences using protease. The masking procedure using PAS was successfully applied to another scFv. The provision to adjust the masking effects of PAS by tuning its length, makes PAS fusion a valuable tool for the universal design of prodrug antibodies." @default.
• W4384502057 created "2023-07-18" @default.
• W4384502057 creator A5002372254 @default.
• W4384502057 creator A5017987959 @default.
• W4384502057 creator A5027793753 @default.
• W4384502057 creator A5051654730 @default.
• W4384502057 creator A5085124300 @default.
• W4384502057 date "2023-11-01" @default.
• W4384502057 modified "2023-10-15" @default.
• W4384502057 title "Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies" @default.
• W4384502057 cites W1744998862 @default.
• W4384502057 cites W1974949597 @default.
• W4384502057 cites W1976752775 @default.
• W4384502057 cites W1984106304 @default.
• W4384502057 cites W1990435383 @default.
• W4384502057 cites W1995043914 @default.
• W4384502057 cites W2005205132 @default.
• W4384502057 cites W2012203161 @default.
• W4384502057 cites W2061072777 @default.
• W4384502057 cites W2066680692 @default.
• W4384502057 cites W2077950012 @default.
• W4384502057 cites W2088729407 @default.
• W4384502057 cites W2101160383 @default.
• W4384502057 cites W2106246537 @default.
• W4384502057 cites W2109851373 @default.
• W4384502057 cites W2147122689 @default.
• W4384502057 cites W2161892929 @default.
• W4384502057 cites W2170996501 @default.
• W4384502057 cites W2508070837 @default.
• W4384502057 cites W2606778219 @default.
• W4384502057 cites W2727454415 @default.
• W4384502057 cites W2754495257 @default.
• W4384502057 cites W2755682216 @default.
• W4384502057 cites W2793641859 @default.
• W4384502057 cites W2805494169 @default.
• W4384502057 cites W2901798378 @default.
• W4384502057 cites W2901968722 @default.
• W4384502057 cites W2918051960 @default.
• W4384502057 cites W2945262398 @default.
• W4384502057 cites W2947338041 @default.
• W4384502057 cites W2948416105 @default.
• W4384502057 cites W2995443117 @default.
• W4384502057 cites W3038079742 @default.
• W4384502057 cites W3158787141 @default.
• W4384502057 cites W3173770245 @default.
• W4384502057 cites W4241343732 @default.
• W4384502057 doi "https://doi.org/10.1016/j.nbt.2023.07.004" @default.
• W4384502057 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37467927" @default.
• W4384502057 hasPublicationYear "2023" @default.
• W4384502057 type Work @default.
• W4384502057 citedByCount "0" @default.
• W4384502057 crossrefType "journal-article" @default.
• W4384502057 hasAuthorship W4384502057A5002372254 @default.
• W4384502057 hasAuthorship W4384502057A5017987959 @default.
• W4384502057 hasAuthorship W4384502057A5027793753 @default.
• W4384502057 hasAuthorship W4384502057A5051654730 @default.
• W4384502057 hasAuthorship W4384502057A5085124300 @default.
• W4384502057 hasBestOaLocation W43845020571 @default.
• W4384502057 hasConcept C153911025 @default.
• W4384502057 hasConcept C159654299 @default.
• W4384502057 hasConcept C181199279 @default.
• W4384502057 hasConcept C182220744 @default.
• W4384502057 hasConcept C185592680 @default.
• W4384502057 hasConcept C195616568 @default.
• W4384502057 hasConcept C203014093 @default.
• W4384502057 hasConcept C2778112365 @default.
• W4384502057 hasConcept C3020495827 @default.
• W4384502057 hasConcept C542903549 @default.
• W4384502057 hasConcept C55493867 @default.
• W4384502057 hasConcept C86803240 @default.
• W4384502057 hasConceptScore W4384502057C153911025 @default.
• W4384502057 hasConceptScore W4384502057C159654299 @default.
• W4384502057 hasConceptScore W4384502057C181199279 @default.
• W4384502057 hasConceptScore W4384502057C182220744 @default.
• W4384502057 hasConceptScore W4384502057C185592680 @default.
• W4384502057 hasConceptScore W4384502057C195616568 @default.
• W4384502057 hasConceptScore W4384502057C203014093 @default.
• W4384502057 hasConceptScore W4384502057C2778112365 @default.
• W4384502057 hasConceptScore W4384502057C3020495827 @default.
• W4384502057 hasConceptScore W4384502057C542903549 @default.
• W4384502057 hasConceptScore W4384502057C55493867 @default.
• W4384502057 hasConceptScore W4384502057C86803240 @default.
• W4384502057 hasFunder F4320311405 @default.
• W4384502057 hasFunder F4320320912 @default.
• W4384502057 hasFunder F4320325836 @default.
• W4384502057 hasFunder F4320334764 @default.
• W4384502057 hasLocation W43845020571 @default.
• W4384502057 hasLocation W43845020572 @default.
• W4384502057 hasOpenAccess W4384502057 @default.
• W4384502057 hasPrimaryLocation W43845020571 @default.
• W4384502057 hasRelatedWork W1979044599 @default.
• W4384502057 hasRelatedWork W2006264959 @default.
• W4384502057 hasRelatedWork W2009867644 @default.
• W4384502057 hasRelatedWork W2021824670 @default.
• W4384502057 hasRelatedWork W2028965232 @default.
• W4384502057 hasRelatedWork W2061945526 @default.
• W4384502057 hasRelatedWork W2357602378 @default.
• W4384502057 hasRelatedWork W2363997565 @default.

• next