FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384562810> ?p ?o ?g. }

• W4384562810 endingPage "104971" @default.
• W4384562810 startingPage "104971" @default.
• W4384562810 abstract "Epidermolytic palmoplantar keratoderma (EPPK), a highly penetrant autosomal dominant genodermatosis, is characterized by diffuse keratoses on palmplantar epidermis. The keratin 9 gene (KRT9) is responsible for EPPK. To date, phenotypic therapy is the primary treatment for EPPK. Because KRT9 pairs with a type II keratin-binding partner to function in epidermis, identifying the interaction partner is an essential first step in revealing EPPK pathogenesis and its fundamental treatment. In this study, we proved that keratin 6C (KRT6C) is a probable hereterodimer partner for KRT9. In silico model for KRT6C/KRT9 shows a typical coiled-coil structure in their 2B domains. Proteomics analysis shows that KRT6C/KRT9 pair is in a densely connected protein-protein interaction network, where proteins participate jointly in regulating cytoskeleton organization and keratinization. This study shows that co-immunoprecipitation coupled with mass spectroscopy and proteomics analysis provide a sensitive approach, which compensates for inevitable inadequacies of anti-keratin 6C antibody and helps discover the probable hereterodimer partner KRT6C for KRT9. The acknowledgement of KRT6C/KRT9 pairwise relationship may help re-classify EPPK and PC-K6c (a milder form of pachyonychia congenita, caused by KRT6C) as a group of hereditary defects at a molecular-based level, and lay foundation for deciphering the keratin network contributing to EPPK and PC-K6c. SIGNIFICANCE OF THE STUDY: What is already known about this topic? KRT9 and KRT6C are disease-causing factors for epidermolytic palmoplantar keratoderma (EPPK) and a milder form of pachyonychia congenita (PC-K6c), respectively. EPPK and PC-K6c have some symptom similarities. Keratins are the major structural proteins in epithelial cells. Each of the type I keratin is matched by a particular type II keratin to assemble a coiled-coil heterodimer. The hereterodimer partner for KRT9 is unknown. What does this study add? We discovered and proved that KRT6C is a probable hereterodimer partner for KRT9 in palmplantar epidermis in a native endogenous environment by using co-immunoprecipitation coupled with mass spectroscopy and proteomics analysis, etc. The proteomics analysis shows that KRT6C/KRT9 keratin pair is in a densely connected protein-protein interaction network, where proteins participate jointly in regulating intermediate filament-based cytoskeleton organization and keratinization processes. What are the implications of this work? The new understanding of probable KRT6C/KRT9 pairwise correlation may help re-classify the genetic cutaneous disorders EPPK and PC-K6c as a group of hereditary defects at a molecular-based level, and lay foundation for pathogenic mechanism research in EPPK and PC-K6c. The densely related network components derived from the proteomic data using Metascape in the study and pairwise regulation fashion of specific keratin pairs should attract more attention in the further explorations when investigators concern the physiological functions of keratins and the pathogenesis of related skin diseases." @default.
• W4384562810 created "2023-07-18" @default.
• W4384562810 creator A5006977076 @default.
• W4384562810 creator A5022719352 @default.
• W4384562810 creator A5047592245 @default.
• W4384562810 creator A5082015443 @default.
• W4384562810 creator A5085350603 @default.
• W4384562810 date "2023-09-01" @default.
• W4384562810 modified "2023-09-26" @default.
• W4384562810 title "Proteomic profiling reveals KRT6C as a probable hereterodimer partner for KRT9: New insights into re-classifying epidermolytic palmoplantar keratoderma (EPPK) and a milder form of pachyonychia congenita (PC-K6c) as a group of genetic cutaneous disorders" @default.
• W4384562810 cites W1528731272 @default.
• W4384562810 cites W1565778932 @default.
• W4384562810 cites W1828555256 @default.
• W4384562810 cites W1861504237 @default.
• W4384562810 cites W1968261842 @default.
• W4384562810 cites W1986223242 @default.
• W4384562810 cites W1994448381 @default.
• W4384562810 cites W2015535653 @default.
• W4384562810 cites W2017384060 @default.
• W4384562810 cites W2020173556 @default.
• W4384562810 cites W2027845234 @default.
• W4384562810 cites W2030369093 @default.
• W4384562810 cites W2033452569 @default.
• W4384562810 cites W2034856955 @default.
• W4384562810 cites W2035583615 @default.
• W4384562810 cites W2038798270 @default.
• W4384562810 cites W2059286922 @default.
• W4384562810 cites W2066104198 @default.
• W4384562810 cites W2069860300 @default.
• W4384562810 cites W2071925187 @default.
• W4384562810 cites W2085593759 @default.
• W4384562810 cites W2086176032 @default.
• W4384562810 cites W2100589138 @default.
• W4384562810 cites W2108567123 @default.
• W4384562810 cites W2118487541 @default.
• W4384562810 cites W2122669551 @default.
• W4384562810 cites W2128443448 @default.
• W4384562810 cites W2129345253 @default.
• W4384562810 cites W2136850043 @default.
• W4384562810 cites W2138424169 @default.
• W4384562810 cites W2141807385 @default.
• W4384562810 cites W2148986438 @default.
• W4384562810 cites W2157924726 @default.
• W4384562810 cites W2159675211 @default.
• W4384562810 cites W2163485494 @default.
• W4384562810 cites W2167898988 @default.
• W4384562810 cites W2170400730 @default.
• W4384562810 cites W2326423512 @default.
• W4384562810 cites W2340074729 @default.
• W4384562810 cites W2469275705 @default.
• W4384562810 cites W2507306980 @default.
• W4384562810 cites W2751260413 @default.
• W4384562810 cites W2772836647 @default.
• W4384562810 cites W2774841852 @default.
• W4384562810 cites W2784357126 @default.
• W4384562810 cites W2802882829 @default.
• W4384562810 cites W2804822363 @default.
• W4384562810 cites W2900569176 @default.
• W4384562810 cites W2917418521 @default.
• W4384562810 cites W2928665623 @default.
• W4384562810 cites W2943960100 @default.
• W4384562810 cites W2971595373 @default.
• W4384562810 cites W2987335406 @default.
• W4384562810 cites W2987934639 @default.
• W4384562810 cites W3198072751 @default.
• W4384562810 cites W4223552703 @default.
• W4384562810 doi "https://doi.org/10.1016/j.jprot.2023.104971" @default.
• W4384562810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37467889" @default.
• W4384562810 hasPublicationYear "2023" @default.
• W4384562810 type Work @default.
• W4384562810 citedByCount "0" @default.
• W4384562810 crossrefType "journal-article" @default.
• W4384562810 hasAuthorship W4384562810A5006977076 @default.
• W4384562810 hasAuthorship W4384562810A5022719352 @default.
• W4384562810 hasAuthorship W4384562810A5047592245 @default.
• W4384562810 hasAuthorship W4384562810A5082015443 @default.
• W4384562810 hasAuthorship W4384562810A5085350603 @default.
• W4384562810 hasConcept C104317684 @default.
• W4384562810 hasConcept C108335385 @default.
• W4384562810 hasConcept C142669718 @default.
• W4384562810 hasConcept C1491633281 @default.
• W4384562810 hasConcept C2778156654 @default.
• W4384562810 hasConcept C2778951404 @default.
• W4384562810 hasConcept C2779335771 @default.
• W4384562810 hasConcept C2779915019 @default.
• W4384562810 hasConcept C46111723 @default.
• W4384562810 hasConcept C54355233 @default.
• W4384562810 hasConcept C78383274 @default.
• W4384562810 hasConcept C81241287 @default.
• W4384562810 hasConcept C86803240 @default.
• W4384562810 hasConceptScore W4384562810C104317684 @default.
• W4384562810 hasConceptScore W4384562810C108335385 @default.
• W4384562810 hasConceptScore W4384562810C142669718 @default.
• W4384562810 hasConceptScore W4384562810C1491633281 @default.
• W4384562810 hasConceptScore W4384562810C2778156654 @default.
• W4384562810 hasConceptScore W4384562810C2778951404 @default.
• W4384562810 hasConceptScore W4384562810C2779335771 @default.
• W4384562810 hasConceptScore W4384562810C2779915019 @default.

• next