Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384627141> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4384627141 endingPage "113" @default.
- W4384627141 startingPage "107" @default.
- W4384627141 abstract "To explore the mechanism of Salidroside regulating the phenotypic transformation of cavernous smooth muscle cells (CCSMCs) in rats.Primary CCSMCs were isolated from male SD rats, cultured in hypoxic environment for 24 hours, and treated with Salidroside at 30 μg/mL. Then the expressions of HIF-1α, platelet-derived growth factor receptor (PDGFR) and phenotypic transformation-related proteins α-SMA and Collagen I were detected by Western blot. The culture system of the CCSMCs was treated with exogenous PDGF-BB at 20 ng/mL for 24 hours, and the effects of Salidroside or PDGFR inhibitor Crenolanib (100 nmol/L) were observed. The expressions of PDGFR, phosphorylated PDGFR, phenotypic transformation-related proteins α-SMA and Collagen I, STAT3, phosphorylated STAT3, STAT5 and phosphorylated STAT5 were determined by Western blot. The intervention effects of Salidroside and/or the overexpression of STAT3 were observed after stimulation of the CCSMCs by exogenous PDGF-BB, followed by detection of the expressions of phenotypic transformation-related proteins α-SMA and Collagen I, STAT3 and phosphorylated STAT3 proteins.The expression of HIF-1α in the CCSMCs was significantly upregulated after cultured in hypoxic environment for 24 hours (P < 0.05), suggesting the successful construction of the hypoxia model of CCSMCs. Meanwhile, the expressions of PDGFRα, PDGFRβ and Collagen I were remarkably increased (all P < 0.05), and that of α-SMA markedly decreased (P < 0.05) in the CCSMCs. However, the expressions of the all the proteins above were significantly inhibited by Salidroside intervention (all P < 0.05). After stimulated by exogenous PDGF-BB for 24 hours, the phosphorylation ratios of PDGFRα, PDGFRβ and STAT3 and the expression of Collagen I were significantly elevated (all P < 0.05), that of α-SMA remarkably reduced (P < 0.05), and all were inhibited by intervention with crenolanib or Salidroside (all P < 0.05). No statistically significant difference was observed in the STAT5 phosphorylation ratio between different groups (P > 0.05). Overexpression of STAT3 in the CCSMCs treated with exogenous PDGF-BB and Salidroside significantly decreased the expression of α-SMA (P < 0.05) and increased that of Collagen I (P < 0.05).Salidroside could improve the phenotypic transformation of CCSMCs in male rats through the PDGFR/STAT3 signaling pathway, which needs further exploration and verification." @default.
- W4384627141 created "2023-07-19" @default.
- W4384627141 creator A5000240387 @default.
- W4384627141 creator A5025412131 @default.
- W4384627141 creator A5044197595 @default.
- W4384627141 creator A5067019176 @default.
- W4384627141 creator A5067451186 @default.
- W4384627141 creator A5079543320 @default.
- W4384627141 date "2022-02-01" @default.
- W4384627141 modified "2023-10-17" @default.
- W4384627141 title "[Salidroside regulates the phenotypic transformation of corpus cavernosum smooth muscle cells of rats through the PDGFR/STAT3 signaling pathway]." @default.
- W4384627141 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37462481" @default.
- W4384627141 hasPublicationYear "2022" @default.
- W4384627141 type Work @default.
- W4384627141 citedByCount "0" @default.
- W4384627141 crossrefType "journal-article" @default.
- W4384627141 hasAuthorship W4384627141A5000240387 @default.
- W4384627141 hasAuthorship W4384627141A5025412131 @default.
- W4384627141 hasAuthorship W4384627141A5044197595 @default.
- W4384627141 hasAuthorship W4384627141A5067019176 @default.
- W4384627141 hasAuthorship W4384627141A5067451186 @default.
- W4384627141 hasAuthorship W4384627141A5079543320 @default.
- W4384627141 hasConcept C104317684 @default.
- W4384627141 hasConcept C11960822 @default.
- W4384627141 hasConcept C126322002 @default.
- W4384627141 hasConcept C127561419 @default.
- W4384627141 hasConcept C134018914 @default.
- W4384627141 hasConcept C153911025 @default.
- W4384627141 hasConcept C170493617 @default.
- W4384627141 hasConcept C180361614 @default.
- W4384627141 hasConcept C185592680 @default.
- W4384627141 hasConcept C2775960820 @default.
- W4384627141 hasConcept C2776415932 @default.
- W4384627141 hasConcept C2778923194 @default.
- W4384627141 hasConcept C2779526882 @default.
- W4384627141 hasConcept C55493867 @default.
- W4384627141 hasConcept C71924100 @default.
- W4384627141 hasConcept C86803240 @default.
- W4384627141 hasConcept C95444343 @default.
- W4384627141 hasConcept C98274493 @default.
- W4384627141 hasConceptScore W4384627141C104317684 @default.
- W4384627141 hasConceptScore W4384627141C11960822 @default.
- W4384627141 hasConceptScore W4384627141C126322002 @default.
- W4384627141 hasConceptScore W4384627141C127561419 @default.
- W4384627141 hasConceptScore W4384627141C134018914 @default.
- W4384627141 hasConceptScore W4384627141C153911025 @default.
- W4384627141 hasConceptScore W4384627141C170493617 @default.
- W4384627141 hasConceptScore W4384627141C180361614 @default.
- W4384627141 hasConceptScore W4384627141C185592680 @default.
- W4384627141 hasConceptScore W4384627141C2775960820 @default.
- W4384627141 hasConceptScore W4384627141C2776415932 @default.
- W4384627141 hasConceptScore W4384627141C2778923194 @default.
- W4384627141 hasConceptScore W4384627141C2779526882 @default.
- W4384627141 hasConceptScore W4384627141C55493867 @default.
- W4384627141 hasConceptScore W4384627141C71924100 @default.
- W4384627141 hasConceptScore W4384627141C86803240 @default.
- W4384627141 hasConceptScore W4384627141C95444343 @default.
- W4384627141 hasConceptScore W4384627141C98274493 @default.
- W4384627141 hasIssue "2" @default.
- W4384627141 hasLocation W43846271411 @default.
- W4384627141 hasOpenAccess W4384627141 @default.
- W4384627141 hasPrimaryLocation W43846271411 @default.
- W4384627141 hasRelatedWork W2020449916 @default.
- W4384627141 hasRelatedWork W2025703417 @default.
- W4384627141 hasRelatedWork W2033472167 @default.
- W4384627141 hasRelatedWork W2034583712 @default.
- W4384627141 hasRelatedWork W2063619316 @default.
- W4384627141 hasRelatedWork W2074821979 @default.
- W4384627141 hasRelatedWork W2126532023 @default.
- W4384627141 hasRelatedWork W2165001775 @default.
- W4384627141 hasRelatedWork W2169225653 @default.
- W4384627141 hasRelatedWork W2169932159 @default.
- W4384627141 hasVolume "28" @default.
- W4384627141 isParatext "false" @default.
- W4384627141 isRetracted "false" @default.
- W4384627141 workType "article" @default.