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- W4384641326 abstract "Lytic polysaccharide monooxygenases (LPMOs) are taxonomically widespread copper-enzymes boosting biopolymers conversion (e.g. cellulose, chitin) in Nature. White-rot Polyporales, which are major fungal wood decayers, may possess up to 60 LPMO-encoding genes belonging to the auxiliary activities family 9 (AA9). Yet, the functional relevance of such multiplicity remains to be uncovered. Previous comparative transcriptomic studies of six Polyporales fungi grown on cellulosic substrates had shown the overexpression of numerous AA9-encoding genes, including some holding a C-terminal domain of unknown function (X282). Here, after carrying out structural predictions and phylogenetic analyses, we selected and characterized six AA9-X282s with different C-term modularities and atypical features hitherto unreported. Unexpectedly, after screening a large array of conditions, these AA9-X282s showed only weak binding properties to cellulose, and low to no cellulolytic oxidative activity. Strikingly, proteomic analysis revealed the presence of multiple phosphorylated residues at the surface of these AA9-X282s, including a conserved residue next to the copper site. Further analyses focusing on a 9 residues glycine-rich C-term extension suggested that it could hold phosphate-binding properties. Our results question the involvement of these AA9 proteins in the degradation of plant cell wall and open new avenues as to the divergence of function of some AA9 members." @default.
- W4384641326 created "2023-07-19" @default.
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- W4384641326 date "2023-07-18" @default.
- W4384641326 modified "2023-10-06" @default.
- W4384641326 title "Insights into peculiar fungal LPMO family members holding a short C-terminal sequence reminiscent of phosphate binding motifs" @default.
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- W4384641326 doi "https://doi.org/10.1038/s41598-023-38617-5" @default.
- W4384641326 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37463979" @default.
- W4384641326 hasPublicationYear "2023" @default.
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