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• W4384661631 abstract "Abstract S100A11 is a small Ca 2+ -activatable protein with an established role in different cellular processes involving actin cytoskeleton remodeling, such as cell migration, membrane protrusion formation, and plasma membrane repair. It also displays Ca 2+ -dependent F-actin binding activity and localizes to actin stress fibers (SFs), but its precise role in regulating these structures remains unclear. Analyzing endogenous S100A11 localization in HeLa and U2OS osteosarcoma cells confirmed SF association but in addition revealed steady localization to stable focal adhesions (FAs), typically at the end of dorsal stress fibers. In contrast, S100A11 levels at FAs increased sharply, but transiently, at the onset of peripheral FA disassembly. Elevating intracellular Ca 2+ levels using the Ca 2+ ionophore ionomycin reliably stimulated both S100A11 recruitment and subsequent FA disassembly. However, pre-incubation with the non-muscle myosin II (NM II) inhibitor blebbistatin, or with an inhibitor to the stretch-activatable Ca 2+ channel Piezo1 effectively suppressed S100A11 recruitment, implicating S100A11 in an actomyosin contractility-driven FA disassembly mechanism involving Piezo1-dependent Ca 2+ influx. Applying external mechanical forces on peripheral FAs via a micropipette likewise recruited S100A11 to FAs, even when NM II was inhibited by blebbistatin or in NM IIA knockout cells, corroborating the mechanosensitive recruitment mechanism of S100A11. However, extracellular Ca 2+ and Piezo1 function was still indispensable, indicating that NM II-dependent contraction forces act upstream of Piezo1-mediated Ca 2+ influx, in turn leading to S100A11 activation and FA recruitment. Moreover, S100A11 knockout cells feature enlarged FAs and display delayed FA disassembly during cell membrane retraction, consistent with impaired FA turnover in these cells. Our results thus demonstrate a novel mechano-sensitive function for S100A11 in promoting actomyosin contractility-driven FA disassembly." @default.
• W4384661631 created "2023-07-20" @default.
• W4384661631 creator A5001460216 @default.
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• W4384661631 date "2023-07-18" @default.
• W4384661631 modified "2023-10-06" @default.
• W4384661631 title "S100A11 promotes focal adhesion disassembly via myosin II-driven contractility and Piezo1-mediated Ca²⁺entry" @default.
• W4384661631 cites W1489754394 @default.
• W4384661631 cites W1524492065 @default.
• W4384661631 cites W1582247730 @default.
• W4384661631 cites W1801147305 @default.
• W4384661631 cites W1932669647 @default.
• W4384661631 cites W1981125423 @default.
• W4384661631 cites W1982149574 @default.
• W4384661631 cites W1982957540 @default.
• W4384661631 cites W1986681332 @default.
• W4384661631 cites W1987704865 @default.
• W4384661631 cites W1988194926 @default.
• W4384661631 cites W1989197126 @default.
• W4384661631 cites W1989802172 @default.
• W4384661631 cites W1990076574 @default.
• W4384661631 cites W1994930910 @default.
• W4384661631 cites W1996908751 @default.
• W4384661631 cites W1998875485 @default.
• W4384661631 cites W1999720993 @default.
• W4384661631 cites W2000068665 @default.
• W4384661631 cites W2005548065 @default.
• W4384661631 cites W2007660746 @default.
• W4384661631 cites W2009024878 @default.
• W4384661631 cites W2010243889 @default.
• W4384661631 cites W2012678565 @default.
• W4384661631 cites W2013327673 @default.
• W4384661631 cites W2020472510 @default.
• W4384661631 cites W2028338446 @default.
• W4384661631 cites W2030506720 @default.
• W4384661631 cites W2031735540 @default.
• W4384661631 cites W2044153685 @default.
• W4384661631 cites W2044635087 @default.
• W4384661631 cites W2045540390 @default.
• W4384661631 cites W2048209624 @default.
• W4384661631 cites W2049193842 @default.
• W4384661631 cites W2050612706 @default.
• W4384661631 cites W2051176222 @default.
• W4384661631 cites W2052378979 @default.
• W4384661631 cites W2060287015 @default.
• W4384661631 cites W2063408699 @default.
• W4384661631 cites W2078628246 @default.
• W4384661631 cites W2081848364 @default.
• W4384661631 cites W2082220504 @default.
• W4384661631 cites W2091713520 @default.
• W4384661631 cites W2099253537 @default.
• W4384661631 cites W2104055042 @default.
• W4384661631 cites W2104155917 @default.
• W4384661631 cites W2110375403 @default.
• W4384661631 cites W2111537434 @default.
• W4384661631 cites W2112924183 @default.
• W4384661631 cites W2115555722 @default.
• W4384661631 cites W2116527577 @default.
• W4384661631 cites W2123404680 @default.
• W4384661631 cites W2132907046 @default.
• W4384661631 cites W2140409748 @default.
• W4384661631 cites W2141075109 @default.
• W4384661631 cites W2149873896 @default.
• W4384661631 cites W2149893493 @default.
• W4384661631 cites W2150102283 @default.
• W4384661631 cites W2154374239 @default.
• W4384661631 cites W2156117706 @default.
• W4384661631 cites W2161888715 @default.
• W4384661631 cites W2166211520 @default.
• W4384661631 cites W2167431292 @default.
• W4384661631 cites W2252568502 @default.
• W4384661631 cites W2398161251 @default.
• W4384661631 cites W2536678184 @default.
• W4384661631 cites W2807660866 @default.
• W4384661631 cites W2966539065 @default.
• W4384661631 cites W2981103703 @default.
• W4384661631 cites W2982515077 @default.
• W4384661631 cites W2999442572 @default.
• W4384661631 cites W3005706188 @default.
• W4384661631 cites W3016891476 @default.
• W4384661631 cites W3046219739 @default.
• W4384661631 cites W3091805122 @default.
• W4384661631 cites W3092934047 @default.
• W4384661631 cites W3111521801 @default.
• W4384661631 cites W3133566696 @default.
• W4384661631 cites W3138593211 @default.
• W4384661631 cites W3168744267 @default.
• W4384661631 cites W3189734502 @default.
• W4384661631 cites W4220732250 @default.
• W4384661631 cites W4248451563 @default.
• W4384661631 cites W4296261100 @default.
• W4384661631 cites W4308648843 @default.

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