FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384661844> ?p ?o ?g. }

• W4384661844 abstract "Tissue-engineered skeletal muscle can play an important role in regenerative medicine, disease modeling, drug testing, as well as the actuation of biohybrid machines. As the applications of engineered muscle tissues expand, there exists a growing need to cryopreserve and store these tissues without impairing function. In a previous study, we developed a cryopreservation protocol in which engineered skeletal muscle tissues are frozen before myogenic differentiation. In that study, we found that this cryopreservation process led to a three-fold increase in the force generation of the differentiated muscle. Here, we perform further testing to determine the mechanisms by which cryopreservation enhances engineered skeletal muscle function. We found that cryopreservation alters the microstructure of the tissue by increasing pore size and decreasing elastic modulus of the extracellular matrix (ECM), which leads to increased expression of genes related to cell migration, cell-matrix adhesion, ECM secretion, and protease activity. Specifically, cryopreservation leads to the upregulation of many ECM proteins, including laminin, fibronectin, and several types of collagens, as well as integrins and matrix metalloproteinases. These changes to ECM structure and composition were associated with enhanced myogenic differentiation, as evidenced by the upregulation of late-stage myogenic markers and increased force generation. These results highlight the need to understand the effects of cryopreservation on the ECM of other tissues as we strive to advance tissue and organ cryopreservation protocols for regenerative medicine. This study examines the effects of cryopreservation on engineered skeletal muscle tissue structure and uncovers the mechanisms by which these changes impact tissue development and function. These insights help inform the engineering of stronger muscle tissues and highlight the need to investigate cryopreservation-mediated changes to extracellular matrix as we develop suitable cryopreservation protocols for other tissues and organs." @default.
• W4384661844 created "2023-07-20" @default.
• W4384661844 creator A5028089864 @default.
• W4384661844 creator A5050715203 @default.
• W4384661844 creator A5083748138 @default.
• W4384661844 creator A5092492254 @default.
• W4384661844 date "2023-08-10" @default.
• W4384661844 modified "2023-09-25" @default.
• W4384661844 title "Cryopreservation Alters Tissue Structure and Improves Differentiation of Engineered Skeletal Muscle" @default.
• W4384661844 cites W1509119336 @default.
• W4384661844 cites W1970253048 @default.
• W4384661844 cites W1993330421 @default.
• W4384661844 cites W1994315682 @default.
• W4384661844 cites W2004048009 @default.
• W4384661844 cites W2004822806 @default.
• W4384661844 cites W2010478953 @default.
• W4384661844 cites W2026406016 @default.
• W4384661844 cites W2042709468 @default.
• W4384661844 cites W2045295282 @default.
• W4384661844 cites W2050875646 @default.
• W4384661844 cites W2052399363 @default.
• W4384661844 cites W2061965964 @default.
• W4384661844 cites W2086491525 @default.
• W4384661844 cites W2100324080 @default.
• W4384661844 cites W2107018762 @default.
• W4384661844 cites W2110065044 @default.
• W4384661844 cites W2114104545 @default.
• W4384661844 cites W2130116522 @default.
• W4384661844 cites W2138207763 @default.
• W4384661844 cites W2145231066 @default.
• W4384661844 cites W2146512944 @default.
• W4384661844 cites W2154279806 @default.
• W4384661844 cites W2167243609 @default.
• W4384661844 cites W2169456326 @default.
• W4384661844 cites W2466314544 @default.
• W4384661844 cites W2571352594 @default.
• W4384661844 cites W2886154388 @default.
• W4384661844 cites W2899689944 @default.
• W4384661844 cites W2926702890 @default.
• W4384661844 cites W2937109287 @default.
• W4384661844 cites W2965537493 @default.
• W4384661844 cites W2990565615 @default.
• W4384661844 cites W3127488895 @default.
• W4384661844 cites W3142324733 @default.
• W4384661844 doi "https://doi.org/10.1089/ten.tea.2023.0075" @default.
• W4384661844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37463097" @default.
• W4384661844 hasPublicationYear "2023" @default.
• W4384661844 type Work @default.
• W4384661844 citedByCount "0" @default.
• W4384661844 crossrefType "journal-article" @default.
• W4384661844 hasAuthorship W4384661844A5028089864 @default.
• W4384661844 hasAuthorship W4384661844A5050715203 @default.
• W4384661844 hasAuthorship W4384661844A5083748138 @default.
• W4384661844 hasAuthorship W4384661844A5092492254 @default.
• W4384661844 hasConcept C105702510 @default.
• W4384661844 hasConcept C10854531 @default.
• W4384661844 hasConcept C179933525 @default.
• W4384661844 hasConcept C185592680 @default.
• W4384661844 hasConcept C189165786 @default.
• W4384661844 hasConcept C196843134 @default.
• W4384661844 hasConcept C198826908 @default.
• W4384661844 hasConcept C2779814568 @default.
• W4384661844 hasConcept C2779959927 @default.
• W4384661844 hasConcept C28328180 @default.
• W4384661844 hasConcept C49892992 @default.
• W4384661844 hasConcept C54355233 @default.
• W4384661844 hasConcept C86492073 @default.
• W4384661844 hasConcept C86803240 @default.
• W4384661844 hasConcept C95444343 @default.
• W4384661844 hasConceptScore W4384661844C105702510 @default.
• W4384661844 hasConceptScore W4384661844C10854531 @default.
• W4384661844 hasConceptScore W4384661844C179933525 @default.
• W4384661844 hasConceptScore W4384661844C185592680 @default.
• W4384661844 hasConceptScore W4384661844C189165786 @default.
• W4384661844 hasConceptScore W4384661844C196843134 @default.
• W4384661844 hasConceptScore W4384661844C198826908 @default.
• W4384661844 hasConceptScore W4384661844C2779814568 @default.
• W4384661844 hasConceptScore W4384661844C2779959927 @default.
• W4384661844 hasConceptScore W4384661844C28328180 @default.
• W4384661844 hasConceptScore W4384661844C49892992 @default.
• W4384661844 hasConceptScore W4384661844C54355233 @default.
• W4384661844 hasConceptScore W4384661844C86492073 @default.
• W4384661844 hasConceptScore W4384661844C86803240 @default.
• W4384661844 hasConceptScore W4384661844C95444343 @default.
• W4384661844 hasLocation W43846618441 @default.
• W4384661844 hasLocation W43846618442 @default.
• W4384661844 hasOpenAccess W4384661844 @default.
• W4384661844 hasPrimaryLocation W43846618441 @default.
• W4384661844 hasRelatedWork W1995698795 @default.
• W4384661844 hasRelatedWork W2034939167 @default.
• W4384661844 hasRelatedWork W2064487804 @default.
• W4384661844 hasRelatedWork W2106761248 @default.
• W4384661844 hasRelatedWork W2115780596 @default.
• W4384661844 hasRelatedWork W2128518557 @default.
• W4384661844 hasRelatedWork W2481695001 @default.
• W4384661844 hasRelatedWork W2606658315 @default.
• W4384661844 hasRelatedWork W2808247336 @default.
• W4384661844 hasRelatedWork W2992394541 @default.
• W4384661844 isParatext "false" @default.
• W4384661844 isRetracted "false" @default.

• next