FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384664568> ?p ?o ?g. }

• W4384664568 abstract "Abstract Background The mechanisms underlying the clinical outcome disparity during human infection with Giardia duodenalis are still unclear. In recent years, evidence has pointed to the roles of host factors as well as parasite’s genetic heterogeneity as major contributing factors in the development of symptomatic human giardiasis. However, it remains contested as to how only a small fraction of individuals infected with G. duodenalis develop clinical gastrointestinal manifestations, whereas the majority of infected individuals remain asymptomatic. Here, we demonstrate that diversity in the fecal microbiome correlates with the clinical outcome of human giardiasis. Methods The genetic heterogeneity of G. duodenalis clinical isolates from human subjects with asymptomatic and symptomatic giardiasis was determined using a multilocus analysis approach. We also assessed the genetic proximity of G. duodenalis isolates by constructing phylogenetic trees using the maximum likelihood. Total genomic DNA (gDNA) from fecal specimens was utilized to construct DNA libraries, followed by performing paired-end sequencing using the HiSeq X platform. The Kraken2-generated, filtered FASTQ files were assigned to microbial metabolic pathways and functions using HUMAnN 3.04 and the UniRef90 diamond annotated full reference database (version 201901b). Results from HUMAnN for each sample were evaluated for differences among the biological groups using the Kruskal–Wallis non-parametric test with a post hoc Dunn test. Results We found that a total of 8/11 (72.73%) human subjects were infected with assemblage A (sub-assemblage AII) of G. duodenalis , whereas 3/11 (27.27%) human subjects in the current study were infected with assemblage B of the parasite. We also found that the parasite’s genetic diversity was not associated with the clinical outcome of the infection. Further phylogenetic analysis based on the tpi and gdh loci indicated that those clinical isolates belonging to assemblage A of G. duodenalis subjects clustered compactly together in a monophyletic clade despite being isolated from human subjects with asymptomatic and symptomatic human giardiasis. Using a metagenomic shotgun sequencing approach, we observed that infected individuals with asymptomatic and symptomatic giardiasis represented distinctive microbial diversity profiles, and that both were distinguishable from the profiles of healthy volunteers. Conclusions These findings identify a potential association between host microbiome disparity with the development of clinical disease during human giardiasis, and may provide insights into the mechanisms by which the parasite induces pathological changes in the gut. These observations may also lead to the development of novel selective therapeutic targets for preventing human enteric microbial infections. Graphical Abstract" @default.
• W4384664568 created "2023-07-20" @default.
• W4384664568 creator A5001817485 @default.
• W4384664568 creator A5002938713 @default.
• W4384664568 creator A5036034644 @default.
• W4384664568 creator A5054777043 @default.
• W4384664568 creator A5055204502 @default.
• W4384664568 creator A5056802504 @default.
• W4384664568 creator A5057110585 @default.
• W4384664568 creator A5068376728 @default.
• W4384664568 creator A5074272261 @default.
• W4384664568 date "2023-07-18" @default.
• W4384664568 modified "2023-10-01" @default.
• W4384664568 title "A shotgun metagenomic analysis of the fecal microbiome in humans infected with Giardia duodenalis" @default.
• W4384664568 cites W1516040977 @default.
• W4384664568 cites W1564662492 @default.
• W4384664568 cites W1925668163 @default.
• W4384664568 cites W1988227922 @default.
• W4384664568 cites W1990792595 @default.
• W4384664568 cites W1991130578 @default.
• W4384664568 cites W1991858217 @default.
• W4384664568 cites W1991864822 @default.
• W4384664568 cites W1992588548 @default.
• W4384664568 cites W2002359269 @default.
• W4384664568 cites W2003006324 @default.
• W4384664568 cites W2015289159 @default.
• W4384664568 cites W2015400330 @default.
• W4384664568 cites W2019858741 @default.
• W4384664568 cites W2021196616 @default.
• W4384664568 cites W2037693477 @default.
• W4384664568 cites W2039784294 @default.
• W4384664568 cites W2063387189 @default.
• W4384664568 cites W2064960064 @default.
• W4384664568 cites W2065300936 @default.
• W4384664568 cites W2071615747 @default.
• W4384664568 cites W2075683777 @default.
• W4384664568 cites W2089600442 @default.
• W4384664568 cites W2100059937 @default.
• W4384664568 cites W2100085650 @default.
• W4384664568 cites W2101478387 @default.
• W4384664568 cites W2105871058 @default.
• W4384664568 cites W2111447050 @default.
• W4384664568 cites W2112845897 @default.
• W4384664568 cites W2117992130 @default.
• W4384664568 cites W2122401826 @default.
• W4384664568 cites W2128591134 @default.
• W4384664568 cites W2128928413 @default.
• W4384664568 cites W2141351365 @default.
• W4384664568 cites W2142118764 @default.
• W4384664568 cites W2142873078 @default.
• W4384664568 cites W2145654450 @default.
• W4384664568 cites W2147043577 @default.
• W4384664568 cites W2149091479 @default.
• W4384664568 cites W2152383952 @default.
• W4384664568 cites W2152977880 @default.
• W4384664568 cites W2166003238 @default.
• W4384664568 cites W2196295400 @default.
• W4384664568 cites W2272600231 @default.
• W4384664568 cites W2395724469 @default.
• W4384664568 cites W2407777116 @default.
• W4384664568 cites W2418146721 @default.
• W4384664568 cites W2473512158 @default.
• W4384664568 cites W2498069089 @default.
• W4384664568 cites W2500688207 @default.
• W4384664568 cites W2526955347 @default.
• W4384664568 cites W258650080 @default.
• W4384664568 cites W2590003621 @default.
• W4384664568 cites W2606004738 @default.
• W4384664568 cites W2735513929 @default.
• W4384664568 cites W2775171339 @default.
• W4384664568 cites W2775793257 @default.
• W4384664568 cites W2793098272 @default.
• W4384664568 cites W2793137450 @default.
• W4384664568 cites W2794306828 @default.
• W4384664568 cites W2811306079 @default.
• W4384664568 cites W2907245946 @default.
• W4384664568 cites W2990182987 @default.
• W4384664568 cites W2990787497 @default.
• W4384664568 cites W2992435003 @default.
• W4384664568 cites W3006367320 @default.
• W4384664568 cites W3016607936 @default.
• W4384664568 cites W3016826587 @default.
• W4384664568 cites W3017326172 @default.
• W4384664568 cites W3046968228 @default.
• W4384664568 cites W3091308661 @default.
• W4384664568 cites W3116608007 @default.
• W4384664568 cites W3121959853 @default.
• W4384664568 cites W3122014285 @default.
• W4384664568 cites W3130485171 @default.
• W4384664568 cites W3138178552 @default.
• W4384664568 cites W3159504416 @default.
• W4384664568 cites W3178732222 @default.
• W4384664568 cites W3199699228 @default.
• W4384664568 cites W3202800434 @default.
• W4384664568 cites W4212914395 @default.
• W4384664568 cites W4293071003 @default.
• W4384664568 cites W4301430887 @default.
• W4384664568 cites W3088458103 @default.
• W4384664568 doi "https://doi.org/10.1186/s13071-023-05821-1" @default.
• W4384664568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37464386" @default.

• next