FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384665048> ?p ?o ?g. }

• W4384665048 abstract "Aromatic compounds derived from tyrosine are important and diverse chemicals that have industrial and commercial applications. Although these aromatic compounds can be obtained by extraction from natural producers, their growth is slow, and their content is low. To overcome these problems, many of them have been chemically synthesized from petroleum-based feedstocks. However, because of the environmental burden and depleting availability of feedstock, microbial cell factories are attracting much attention as sustainable and environmentally friendly processes.To facilitate development of microbial cell factories for producing tyrosine derivatives, we developed simple and convenient tyrosine-producing Escherichia coli platforms with a bacterial phenylalanine hydroxylase, which converted phenylalanine to tyrosine with tetrahydromonapterin as a cofactor, using a synthetic biology approach. By introducing a tetrahydrobiopterin-regeneration system, the tyrosine titer of the plasmid-based engineered strain was 4.63 g/L in a medium supplemented with 5.00 g/L phenylalanine with a test tube. The strains were successfully used to produce industrially attractive compounds, such as tyrosol with a yield of 1.58 g/L by installing a tyrosol-producing module consisting of genes encoding tyrosine decarboxylase and tyramine oxidase on a plasmid. Gene integration into E. coli chromosomes has an advantage over the use of plasmids because it increases genetic stability without antibiotic feeding to the culture media and enables more flexible pathway engineering by accepting more plasmids with artificial pathway genes. Therefore, we constructed a plasmid-free tyrosine-producing platform by integrating five modules, comprising genes encoding the phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system, into the chromosome. The platform strain could produce 1.04 g/L of 3,4-dihydroxyphenylalanine, a drug medicine, by installing a gene encoding tyrosine hydroxylase and the tetrahydrobiopterin-regeneration system on a plasmid. Moreover, by installing the tyrosol-producing module, tyrosol was produced with a yield of 1.28 g/L.We developed novel E. coli platforms for producing tyrosine from phenylalanine at multi-gram-per-liter levels in test-tube cultivation. The platforms allowed development and evaluation of microbial cell factories installing various designed tyrosine-derivative biosynthetic pathways at multi-grams-per-liter levels in test tubes." @default.
• W4384665048 created "2023-07-20" @default.
• W4384665048 creator A5020322004 @default.
• W4384665048 creator A5027685680 @default.
• W4384665048 creator A5051827440 @default.
• W4384665048 creator A5060438396 @default.
• W4384665048 creator A5084055477 @default.
• W4384665048 date "2023-07-18" @default.
• W4384665048 modified "2023-10-06" @default.
• W4384665048 title "Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system" @default.
• W4384665048 cites W1461106611 @default.
• W4384665048 cites W1537398925 @default.
• W4384665048 cites W1565664047 @default.
• W4384665048 cites W1620635102 @default.
• W4384665048 cites W1965813327 @default.
• W4384665048 cites W1966949519 @default.
• W4384665048 cites W1971668591 @default.
• W4384665048 cites W2010238323 @default.
• W4384665048 cites W2026200455 @default.
• W4384665048 cites W2028054553 @default.
• W4384665048 cites W2052024371 @default.
• W4384665048 cites W2052390632 @default.
• W4384665048 cites W2060238294 @default.
• W4384665048 cites W2080365353 @default.
• W4384665048 cites W2092173381 @default.
• W4384665048 cites W2105879208 @default.
• W4384665048 cites W2111967267 @default.
• W4384665048 cites W2116137883 @default.
• W4384665048 cites W2132793695 @default.
• W4384665048 cites W2135073823 @default.
• W4384665048 cites W2141415674 @default.
• W4384665048 cites W2149828455 @default.
• W4384665048 cites W2164125086 @default.
• W4384665048 cites W2166323054 @default.
• W4384665048 cites W2220904888 @default.
• W4384665048 cites W2313182027 @default.
• W4384665048 cites W2402262832 @default.
• W4384665048 cites W2751832049 @default.
• W4384665048 cites W2771250426 @default.
• W4384665048 cites W2776707578 @default.
• W4384665048 cites W2793430004 @default.
• W4384665048 cites W2794978358 @default.
• W4384665048 cites W2806388399 @default.
• W4384665048 cites W2912479692 @default.
• W4384665048 cites W2921762606 @default.
• W4384665048 cites W2967765437 @default.
• W4384665048 cites W2979985476 @default.
• W4384665048 cites W3000375708 @default.
• W4384665048 cites W3005141077 @default.
• W4384665048 cites W3016422274 @default.
• W4384665048 cites W3020978136 @default.
• W4384665048 cites W3024837609 @default.
• W4384665048 cites W3036284296 @default.
• W4384665048 cites W3174957982 @default.
• W4384665048 cites W3177041780 @default.
• W4384665048 cites W3209642573 @default.
• W4384665048 cites W4224217293 @default.
• W4384665048 cites W4285587068 @default.
• W4384665048 doi "https://doi.org/10.1186/s13068-023-02365-5" @default.
• W4384665048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37464414" @default.
• W4384665048 hasPublicationYear "2023" @default.
• W4384665048 type Work @default.
• W4384665048 citedByCount "0" @default.
• W4384665048 crossrefType "journal-article" @default.
• W4384665048 hasAuthorship W4384665048A5020322004 @default.
• W4384665048 hasAuthorship W4384665048A5027685680 @default.
• W4384665048 hasAuthorship W4384665048A5051827440 @default.
• W4384665048 hasAuthorship W4384665048A5060438396 @default.
• W4384665048 hasAuthorship W4384665048A5084055477 @default.
• W4384665048 hasBestOaLocation W43846650481 @default.
• W4384665048 hasConcept C104317684 @default.
• W4384665048 hasConcept C159167319 @default.
• W4384665048 hasConcept C181199279 @default.
• W4384665048 hasConcept C185592680 @default.
• W4384665048 hasConcept C197957613 @default.
• W4384665048 hasConcept C207009774 @default.
• W4384665048 hasConcept C22744801 @default.
• W4384665048 hasConcept C2776145790 @default.
• W4384665048 hasConcept C2776165026 @default.
• W4384665048 hasConcept C2777431362 @default.
• W4384665048 hasConcept C515207424 @default.
• W4384665048 hasConcept C547475151 @default.
• W4384665048 hasConcept C55493867 @default.
• W4384665048 hasConcept C6350086 @default.
• W4384665048 hasConcept C86803240 @default.
• W4384665048 hasConceptScore W4384665048C104317684 @default.
• W4384665048 hasConceptScore W4384665048C159167319 @default.
• W4384665048 hasConceptScore W4384665048C181199279 @default.
• W4384665048 hasConceptScore W4384665048C185592680 @default.
• W4384665048 hasConceptScore W4384665048C197957613 @default.
• W4384665048 hasConceptScore W4384665048C207009774 @default.
• W4384665048 hasConceptScore W4384665048C22744801 @default.
• W4384665048 hasConceptScore W4384665048C2776145790 @default.
• W4384665048 hasConceptScore W4384665048C2776165026 @default.
• W4384665048 hasConceptScore W4384665048C2777431362 @default.
• W4384665048 hasConceptScore W4384665048C515207424 @default.
• W4384665048 hasConceptScore W4384665048C547475151 @default.
• W4384665048 hasConceptScore W4384665048C55493867 @default.
• W4384665048 hasConceptScore W4384665048C6350086 @default.
• W4384665048 hasConceptScore W4384665048C86803240 @default.

• next