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• W4384694552 abstract "Abstract Background: Quality and Quantity (QQ) culture media was shown a promising effect in enhancing the vasculogenesis of mononuclear cells (MNCs) of healthy volunteers and chronic limb-threatening ischemia (CLTI) patients. In this study, the MNCs from CLTI patients were further investigated based of their risk factors. Methods: In this study, MNCs from chronic limb-threatening ischemia (CLTI) patients with coexisting diabetes mellitus (DM), hypertension (HT), current smoker status, or chronic kidney disease (CKD) stage 3 or above were cultured in QQ culture media, and then investigated for angiogenesis-related phenotype and function. CLTI patients with DM, HT, current smoker status, or CKD were prospectively recruited. Forty-eight patients (mean age: 67.5±8.0 years) were included. DM, HT, current smoker status, and CKD was found in 34 (71.0%), 39 (81.0%), 27(56.3%), and 32 (66.7%) patients, respectively. Results: In CLI patients with coexisting diseases, the percentages of CD34+, CD133+, CD34+ CD133+ progenitor cells; CD 206+ cells; colony forming cells; and, tube formation were significantly higher in the PBMNCs cultured in QQ media than in the PBMNCs cultured in standard culture media. However, the percentage of CD4+ CD25+ CD127+ cells was significantly lower in PBMNCs cultured in QQ culture media compared to the percentage in PBMNCs cultured in standard culture media. Conclusions: Quality and Quantity (QQ)culture media was shown to effectively restore the number of vascular progenitor cells and the vasculogenic function of mononuclear cells from chronic limb-threatening ischemia patients with coexisting diabetes mellitus, hypertension, current smoker status, or chronic kidney disease stage 3 or above." @default.
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• W4384694552 date "2023-07-18" @default.
• W4384694552 modified "2023-09-23" @default.
• W4384694552 title "Quality and Quantity culture media effectively restored the number and vasculogenic function of mononuclear cells from chronic limb- threatening ischemia patients" @default.
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• W4384694552 doi "https://doi.org/10.21203/rs.3.rs-3166262/v1" @default.
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