Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384701650> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4384701650 endingPage "147647" @default.
- W4384701650 startingPage "147647" @default.
- W4384701650 abstract "The ongoing development of assisted reproductive technologies has provided hope to individuals struggling with infertility, promising the potential for a healthy pregnancy. One significant innovation in field of pre-implantation genetic screening (PGS) requires the biopsy of embryos or oocytes, which has potential implications for the health and development of the resultant offspring. Therefore, a non-invasive approach to preimplantation genetic screening is highly sought after. The clinical application of non-invasive preimplantation genetic testing (ni-PGT) is currently limited, with its sensitivity and specificity requiring further investigation. In this study, we used 218 human embryos for single-cell whole genome amplification (WGA), along with ni-PGT of blastocoele fluid (BF) and spent culture medium (SCM). Whole blastocyst (WB), trophectoderm biopsy (TB), and inner cell mass (ICM) from embryo biopsies were used as controls to track genomic signal alterations. Our results showed that the overall genome similarity between SCM and ICM was higher than that of BF. Apart from the Y chromosome, both SCM and ICM demonstrated numerous variant sites across other chromosomes.Further categorization of gene variants in these two sample types revealed that missense variants were the most prevalent, single nucleotide polymorphisms were more common than insertions or deletions, and C > T was the dominant single nucleotide variants in both ICM and SCM. Lastly, we found that the mutant genes in SCM and ICM had different biological functions and pathways. This study indicates that SCM provides a more effective source of embryonic DNA for preimplantation genetic screening, offering a novel reference point for genetic screening research." @default.
- W4384701650 created "2023-07-20" @default.
- W4384701650 creator A5016806564 @default.
- W4384701650 creator A5029112508 @default.
- W4384701650 creator A5036317252 @default.
- W4384701650 creator A5041628263 @default.
- W4384701650 creator A5044773121 @default.
- W4384701650 creator A5050998366 @default.
- W4384701650 creator A5060605576 @default.
- W4384701650 creator A5061651333 @default.
- W4384701650 creator A5075669662 @default.
- W4384701650 creator A5090522057 @default.
- W4384701650 creator A5092495222 @default.
- W4384701650 date "2023-10-01" @default.
- W4384701650 modified "2023-10-16" @default.
- W4384701650 title "SCM is potential resource for non-invasive preimplantation genetic testing based on human embryos single-cell sequencing" @default.
- W4384701650 cites W1536423136 @default.
- W4384701650 cites W1990756988 @default.
- W4384701650 cites W2103441770 @default.
- W4384701650 cites W2103912979 @default.
- W4384701650 cites W2104006121 @default.
- W4384701650 cites W2108234281 @default.
- W4384701650 cites W2140844378 @default.
- W4384701650 cites W2945858728 @default.
- W4384701650 cites W2991431315 @default.
- W4384701650 cites W3031291303 @default.
- W4384701650 cites W3086736880 @default.
- W4384701650 cites W3093103135 @default.
- W4384701650 cites W3098444464 @default.
- W4384701650 cites W3107527779 @default.
- W4384701650 cites W4212922174 @default.
- W4384701650 doi "https://doi.org/10.1016/j.gene.2023.147647" @default.
- W4384701650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37473972" @default.
- W4384701650 hasPublicationYear "2023" @default.
- W4384701650 type Work @default.
- W4384701650 citedByCount "0" @default.
- W4384701650 crossrefType "journal-article" @default.
- W4384701650 hasAuthorship W4384701650A5016806564 @default.
- W4384701650 hasAuthorship W4384701650A5029112508 @default.
- W4384701650 hasAuthorship W4384701650A5036317252 @default.
- W4384701650 hasAuthorship W4384701650A5041628263 @default.
- W4384701650 hasAuthorship W4384701650A5044773121 @default.
- W4384701650 hasAuthorship W4384701650A5050998366 @default.
- W4384701650 hasAuthorship W4384701650A5060605576 @default.
- W4384701650 hasAuthorship W4384701650A5061651333 @default.
- W4384701650 hasAuthorship W4384701650A5075669662 @default.
- W4384701650 hasAuthorship W4384701650A5090522057 @default.
- W4384701650 hasAuthorship W4384701650A5092495222 @default.
- W4384701650 hasConcept C104317684 @default.
- W4384701650 hasConcept C135763542 @default.
- W4384701650 hasConcept C153209595 @default.
- W4384701650 hasConcept C159399396 @default.
- W4384701650 hasConcept C196843134 @default.
- W4384701650 hasConcept C2778177303 @default.
- W4384701650 hasConcept C2778805741 @default.
- W4384701650 hasConcept C54355233 @default.
- W4384701650 hasConcept C86803240 @default.
- W4384701650 hasConcept C87073359 @default.
- W4384701650 hasConceptScore W4384701650C104317684 @default.
- W4384701650 hasConceptScore W4384701650C135763542 @default.
- W4384701650 hasConceptScore W4384701650C153209595 @default.
- W4384701650 hasConceptScore W4384701650C159399396 @default.
- W4384701650 hasConceptScore W4384701650C196843134 @default.
- W4384701650 hasConceptScore W4384701650C2778177303 @default.
- W4384701650 hasConceptScore W4384701650C2778805741 @default.
- W4384701650 hasConceptScore W4384701650C54355233 @default.
- W4384701650 hasConceptScore W4384701650C86803240 @default.
- W4384701650 hasConceptScore W4384701650C87073359 @default.
- W4384701650 hasFunder F4320321001 @default.
- W4384701650 hasLocation W43847016501 @default.
- W4384701650 hasLocation W43847016502 @default.
- W4384701650 hasOpenAccess W4384701650 @default.
- W4384701650 hasPrimaryLocation W43847016501 @default.
- W4384701650 hasRelatedWork W1976299450 @default.
- W4384701650 hasRelatedWork W2066630460 @default.
- W4384701650 hasRelatedWork W2070304098 @default.
- W4384701650 hasRelatedWork W2131540990 @default.
- W4384701650 hasRelatedWork W2142841390 @default.
- W4384701650 hasRelatedWork W2151027856 @default.
- W4384701650 hasRelatedWork W2163567889 @default.
- W4384701650 hasRelatedWork W2763146116 @default.
- W4384701650 hasRelatedWork W2903529700 @default.
- W4384701650 hasRelatedWork W3092588792 @default.
- W4384701650 hasVolume "882" @default.
- W4384701650 isParatext "false" @default.
- W4384701650 isRetracted "false" @default.
- W4384701650 workType "article" @default.