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- W4384818690 abstract "Abstract Background The temporomandibular joint (TMJ) is a complex joint consisting of the condyle, the temporal articular surface, and the articular disc. Functions such as mastication, swallowing and articulation are accomplished by the movements of the TMJ. To date, the TMJ has been studied more extensively, but the types of TMJ cells, their differentiation, and their interrelationship during growth and development are still unclear and the study of the TMJ is limited. The aim of this study was to establish a molecular cellular atlas of the human embryonic temporomandibular joint condyle (TMJC) by single-cell RNA sequencing, which will contribute to understanding and solving clinical problems. Results Human embryos at 3 and 4 months of age are an important stage of TMJC development. We performed a comprehensive transcriptome analysis of TMJC tissue from human embryos at 3 and 4 months of age using single-cell RNA sequencing. A total of 16,624 cells were captured and the gene expression profiles of 15 cell clusters in human embryonic TMJC were determined, including 14 known cell types and one previously unknown cell type, transition state cells (TSCs). Immunofluorescence assays confirmed that TSCs are not the same cell cluster as mesenchymal stem cells (MSCs). Pseudotime trajectory and RNA velocity analysis revealed that MSCs transformed into TSCs, which further differentiated into osteoblasts, hypertrophic chondrocytes and tenocytes. In addition, chondrocytes (CYTL1 high + THBS1 high ) from secondary cartilage were detected only in 4-month-old human embryonic TMJC. Conclusions Our study provides an atlas of differentiation stages of human embryonic TMJC tissue cells, which will contribute to an in-depth understanding of the pathophysiology of the TMJC tissue repair process and ultimately help to solve clinical problems." @default.
- W4384818690 created "2023-07-21" @default.
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- W4384818690 date "2023-07-19" @default.
- W4384818690 modified "2023-10-14" @default.
- W4384818690 title "Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos" @default.
- W4384818690 cites W1157407587 @default.
- W4384818690 cites W1498905387 @default.
- W4384818690 cites W1882140205 @default.
- W4384818690 cites W1985159137 @default.
- W4384818690 cites W1989408064 @default.
- W4384818690 cites W1996576213 @default.
- W4384818690 cites W1997747619 @default.
- W4384818690 cites W1997889647 @default.
- W4384818690 cites W2001890173 @default.
- W4384818690 cites W2003540533 @default.
- W4384818690 cites W2014680491 @default.
- W4384818690 cites W2015050458 @default.
- W4384818690 cites W2035618305 @default.
- W4384818690 cites W2042749121 @default.
- W4384818690 cites W2067226341 @default.
- W4384818690 cites W2080985985 @default.
- W4384818690 cites W2089467986 @default.
- W4384818690 cites W2090060351 @default.
- W4384818690 cites W2093791676 @default.
- W4384818690 cites W2096874451 @default.
- W4384818690 cites W2100039503 @default.
- W4384818690 cites W2119177231 @default.
- W4384818690 cites W2122367477 @default.
- W4384818690 cites W2139055053 @default.
- W4384818690 cites W2149193631 @default.
- W4384818690 cites W2150134228 @default.
- W4384818690 cites W2153693049 @default.
- W4384818690 cites W2154656849 @default.
- W4384818690 cites W2156656278 @default.
- W4384818690 cites W2159023535 @default.
- W4384818690 cites W2159675211 @default.
- W4384818690 cites W2165923850 @default.
- W4384818690 cites W2413134229 @default.
- W4384818690 cites W2431942788 @default.
- W4384818690 cites W2531652035 @default.
- W4384818690 cites W2532248581 @default.
- W4384818690 cites W2563668803 @default.
- W4384818690 cites W2610723828 @default.
- W4384818690 cites W2616922646 @default.
- W4384818690 cites W2737703356 @default.
- W4384818690 cites W2769275789 @default.
- W4384818690 cites W2791033272 @default.
- W4384818690 cites W2805897158 @default.
- W4384818690 cites W2883053515 @default.
- W4384818690 cites W2890455606 @default.
- W4384818690 cites W2890725238 @default.
- W4384818690 cites W2935451440 @default.
- W4384818690 cites W2936519136 @default.
- W4384818690 cites W2944496703 @default.
- W4384818690 cites W2949177718 @default.
- W4384818690 cites W2949243209 @default.
- W4384818690 cites W2951682736 @default.
- W4384818690 cites W2980686444 @default.
- W4384818690 cites W3008123732 @default.
- W4384818690 cites W3011786639 @default.
- W4384818690 cites W3024987148 @default.
- W4384818690 cites W3025897174 @default.
- W4384818690 cites W3037234537 @default.
- W4384818690 cites W3043357509 @default.
- W4384818690 cites W3087485334 @default.
- W4384818690 cites W3092971373 @default.
- W4384818690 cites W3106120778 @default.
- W4384818690 cites W3108216847 @default.
- W4384818690 cites W3132139537 @default.
- W4384818690 cites W3132661792 @default.
- W4384818690 cites W3134788761 @default.
- W4384818690 cites W3139082227 @default.
- W4384818690 cites W3160543329 @default.
- W4384818690 cites W3162473960 @default.
- W4384818690 cites W3165226071 @default.
- W4384818690 cites W3166034714 @default.
- W4384818690 cites W3171420350 @default.
- W4384818690 cites W3185462684 @default.
- W4384818690 cites W3194665116 @default.
- W4384818690 cites W3197480694 @default.
- W4384818690 cites W3202333780 @default.
- W4384818690 cites W3212740460 @default.