Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384926534> ?p ?o ?g. }
- W4384926534 abstract "Nucleation and growth of amyloid fibrils were found to only occur in supersaturated solutions above a critical concentration (ccrit ). The biophysical meaning of ccrit remained mostly obscure, since typical low values of ccrit in the sub-μM range hamper investigations of potential oligomeric states and their structure. Here, we investigate the parathyroid hormone PTH84 as an example of a functional amyloid fibril forming peptide with a comparably high ccrit of 67±21 μM. We describe a complex concentration dependent prenucleation ensemble of oligomers of different sizes and secondary structure compositions and highlight the occurrence of a trimer and tetramer at ccrit as possible precursors for primary fibril nucleation. Furthermore, the soluble state found in equilibrium with fibrils adopts to the prenucleation state present at ccrit . Our study sheds light onto early events of amyloid formation directly related to the critical concentration and underlines oligomer formation as a key feature of fibril nucleation. Our results contribute to a deeper understanding of the determinants of supersaturated peptide solutions. In the current study we present a biophysical approach to investigate ccrit of amyloid fibril formation of PTH84 in terms of secondary structure, cluster size and residue resolved intermolecular interactions during oligomer formation. Throughout the investigated range of concentrations (1 μM to 500 μM) we found different states of oligomerization with varying ability to contribute to primary fibril nucleation and with a concentration dependent equilibrium. In this context, we identified the previously described ccrit of PTH84 to mark a minimum concentration for the formation of homo-trimers/tetramers. These investigations allowed us to characterize molecular interactions of various oligomeric states that are further converted into elongation competent fibril nuclei during the lag phase of a functional amyloid forming peptide." @default.
- W4384926534 created "2023-07-22" @default.
- W4384926534 creator A5009744009 @default.
- W4384926534 creator A5039256435 @default.
- W4384926534 creator A5063160681 @default.
- W4384926534 creator A5069913264 @default.
- W4384926534 creator A5073272119 @default.
- W4384926534 creator A5077620732 @default.
- W4384926534 creator A5087568097 @default.
- W4384926534 date "2023-08-03" @default.
- W4384926534 modified "2023-10-14" @default.
- W4384926534 title "The Prenucleation Equilibrium of the Parathyroid Hormone Determines the Critical Aggregation Concentration and Amyloid Fibril Nucleation" @default.
- W4384926534 cites W1588979652 @default.
- W4384926534 cites W1970163704 @default.
- W4384926534 cites W1981061244 @default.
- W4384926534 cites W1981346588 @default.
- W4384926534 cites W1984569087 @default.
- W4384926534 cites W2001842463 @default.
- W4384926534 cites W2005072201 @default.
- W4384926534 cites W2010462701 @default.
- W4384926534 cites W2011825508 @default.
- W4384926534 cites W2012591531 @default.
- W4384926534 cites W2014020640 @default.
- W4384926534 cites W2021785060 @default.
- W4384926534 cites W2035546135 @default.
- W4384926534 cites W2037800112 @default.
- W4384926534 cites W2046093999 @default.
- W4384926534 cites W2056216432 @default.
- W4384926534 cites W2059804089 @default.
- W4384926534 cites W2061627162 @default.
- W4384926534 cites W2061657038 @default.
- W4384926534 cites W2063237839 @default.
- W4384926534 cites W2063827082 @default.
- W4384926534 cites W2066011752 @default.
- W4384926534 cites W2068722030 @default.
- W4384926534 cites W2074770586 @default.
- W4384926534 cites W2078873812 @default.
- W4384926534 cites W2080586259 @default.
- W4384926534 cites W2083150508 @default.
- W4384926534 cites W2084761689 @default.
- W4384926534 cites W2085467932 @default.
- W4384926534 cites W2089618587 @default.
- W4384926534 cites W2091030000 @default.
- W4384926534 cites W2095320977 @default.
- W4384926534 cites W2096601751 @default.
- W4384926534 cites W2103786212 @default.
- W4384926534 cites W2114471971 @default.
- W4384926534 cites W2115959204 @default.
- W4384926534 cites W2131823126 @default.
- W4384926534 cites W2157448371 @default.
- W4384926534 cites W2161876271 @default.
- W4384926534 cites W2165186407 @default.
- W4384926534 cites W2169821755 @default.
- W4384926534 cites W2171413096 @default.
- W4384926534 cites W2180631471 @default.
- W4384926534 cites W2234046941 @default.
- W4384926534 cites W2235008797 @default.
- W4384926534 cites W2282846220 @default.
- W4384926534 cites W2313020666 @default.
- W4384926534 cites W2318823565 @default.
- W4384926534 cites W2468070683 @default.
- W4384926534 cites W2767075366 @default.
- W4384926534 cites W2782612584 @default.
- W4384926534 cites W2785884569 @default.
- W4384926534 cites W2786469974 @default.
- W4384926534 cites W2788744596 @default.
- W4384926534 cites W2807999222 @default.
- W4384926534 cites W2817784260 @default.
- W4384926534 cites W2884749741 @default.
- W4384926534 cites W2887996311 @default.
- W4384926534 cites W2890885736 @default.
- W4384926534 cites W2902348806 @default.
- W4384926534 cites W2913970828 @default.
- W4384926534 cites W2952029520 @default.
- W4384926534 cites W2976197779 @default.
- W4384926534 cites W3000646254 @default.
- W4384926534 cites W3009683915 @default.
- W4384926534 cites W3011713314 @default.
- W4384926534 cites W3017879639 @default.
- W4384926534 cites W3033124231 @default.
- W4384926534 cites W3037947354 @default.
- W4384926534 cites W3127602688 @default.
- W4384926534 cites W3138353707 @default.
- W4384926534 cites W3177828909 @default.
- W4384926534 cites W3191624148 @default.
- W4384926534 cites W3198280879 @default.
- W4384926534 cites W3211795435 @default.
- W4384926534 cites W4200418099 @default.
- W4384926534 cites W4205192252 @default.
- W4384926534 cites W4213208821 @default.
- W4384926534 cites W4244736178 @default.
- W4384926534 cites W4255420656 @default.
- W4384926534 cites W4285090270 @default.
- W4384926534 cites W4288722968 @default.
- W4384926534 cites W4296162969 @default.
- W4384926534 cites W4318166358 @default.
- W4384926534 cites W4321480573 @default.
- W4384926534 cites W4366083630 @default.
- W4384926534 doi "https://doi.org/10.1002/cphc.202300439" @default.
- W4384926534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37477386" @default.