FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384927997> ?p ?o ?g. }

• W4384927997 abstract "Abstract Leaf plastids harbor a plethora of biochemical reactions including photosynthesis, the most important pathway on earth. Scientists are eager to unveil the physiological processes within the organelle but also their interconnection with the rest of the plant cell. An increasingly important feature of this venture is to use experimental data in the design of metabolic models. A remaining obstacle has been the limited in situ volume information of plastids and other cell organelles. To fill this gap for chloroplasts, we established three microscopy protocols delivering in situ volumes based on: 1) chlorophyll florescence emerging from the thylakoid membrane, 2) a CFP marker embedded in the envelope, and 3) calculations from serial block-face scanning electron microscopy (SBFSEM). The obtained data were corroborated by comparing wild-type data with two mutant lines affected in the plastid division machinery known to produce small and large mesophyll chloroplasts, respectively. Furthermore, we also determined the volume of the much smaller guard cell plastids. Interestingly, their volume is not governed by the same components of the division machinery which defines mesophyll plastid size. Based on our three approaches the average volume of a mature Col-0 wild-type mesophyll chloroplasts is 93 µm 3 . Wild-type guard cell plastids are approximately 18 µm 3 . Lastly, our comparative analysis shows that the chlorophyll florescence analysis can accurately determine chloroplast volumes, providing an important tool to research groups without access to transgenic marker lines expressing genetically encoded fluorescence proteins or high-end costly SBFSEM equipment. Keywords One-sentence summary This work describes and compares three different strategies to obtain accurate volumes of leaf plastids from Arabidopsis, the most widely used model plant." @default.
• W4384927997 created "2023-07-22" @default.
• W4384927997 creator A5019908574 @default.
• W4384927997 creator A5033544243 @default.
• W4384927997 creator A5059789738 @default.
• W4384927997 creator A5060258733 @default.
• W4384927997 date "2023-07-20" @default.
• W4384927997 modified "2023-09-25" @default.
• W4384927997 title "Probing the<i>in-situ</i>volumes of Arabidopsis leaf plastids using 3D confocal and scanning electron microscopy" @default.
• W4384927997 cites W1974627990 @default.
• W4384927997 cites W2011392944 @default.
• W4384927997 cites W2020601582 @default.
• W4384927997 cites W2034213941 @default.
• W4384927997 cites W2038665424 @default.
• W4384927997 cites W2042569778 @default.
• W4384927997 cites W2043262641 @default.
• W4384927997 cites W2062893362 @default.
• W4384927997 cites W2082335522 @default.
• W4384927997 cites W2106222171 @default.
• W4384927997 cites W2109608997 @default.
• W4384927997 cites W2112760670 @default.
• W4384927997 cites W2119697258 @default.
• W4384927997 cites W2144191386 @default.
• W4384927997 cites W2157060317 @default.
• W4384927997 cites W2162560512 @default.
• W4384927997 cites W2167757759 @default.
• W4384927997 cites W2169547217 @default.
• W4384927997 cites W2171614076 @default.
• W4384927997 cites W2489657536 @default.
• W4384927997 cites W2564694626 @default.
• W4384927997 cites W2573000119 @default.
• W4384927997 cites W2607037660 @default.
• W4384927997 cites W2680176724 @default.
• W4384927997 cites W2750794258 @default.
• W4384927997 cites W2756544308 @default.
• W4384927997 cites W2766357374 @default.
• W4384927997 cites W2791850155 @default.
• W4384927997 cites W2793721279 @default.
• W4384927997 cites W2796441808 @default.
• W4384927997 cites W2804339579 @default.
• W4384927997 cites W2976341951 @default.
• W4384927997 cites W3022053753 @default.
• W4384927997 cites W3115251531 @default.
• W4384927997 cites W3132433840 @default.
• W4384927997 cites W3174785266 @default.
• W4384927997 cites W3196363549 @default.
• W4384927997 cites W4256596560 @default.
• W4384927997 cites W4297458108 @default.
• W4384927997 doi "https://doi.org/10.1101/2023.07.16.549198" @default.
• W4384927997 hasPublicationYear "2023" @default.
• W4384927997 type Work @default.
• W4384927997 citedByCount "0" @default.
• W4384927997 crossrefType "posted-content" @default.
• W4384927997 hasAuthorship W4384927997A5019908574 @default.
• W4384927997 hasAuthorship W4384927997A5033544243 @default.
• W4384927997 hasAuthorship W4384927997A5059789738 @default.
• W4384927997 hasAuthorship W4384927997A5060258733 @default.
• W4384927997 hasBestOaLocation W43849279971 @default.
• W4384927997 hasConcept C101692577 @default.
• W4384927997 hasConcept C104317684 @default.
• W4384927997 hasConcept C123828725 @default.
• W4384927997 hasConcept C12554922 @default.
• W4384927997 hasConcept C136547172 @default.
• W4384927997 hasConcept C143065580 @default.
• W4384927997 hasConcept C168240541 @default.
• W4384927997 hasConcept C183688256 @default.
• W4384927997 hasConcept C2775988993 @default.
• W4384927997 hasConcept C2776373379 @default.
• W4384927997 hasConcept C55493867 @default.
• W4384927997 hasConcept C59822182 @default.
• W4384927997 hasConcept C69305403 @default.
• W4384927997 hasConcept C86803240 @default.
• W4384927997 hasConcept C93829228 @default.
• W4384927997 hasConcept C95444343 @default.
• W4384927997 hasConceptScore W4384927997C101692577 @default.
• W4384927997 hasConceptScore W4384927997C104317684 @default.
• W4384927997 hasConceptScore W4384927997C123828725 @default.
• W4384927997 hasConceptScore W4384927997C12554922 @default.
• W4384927997 hasConceptScore W4384927997C136547172 @default.
• W4384927997 hasConceptScore W4384927997C143065580 @default.
• W4384927997 hasConceptScore W4384927997C168240541 @default.
• W4384927997 hasConceptScore W4384927997C183688256 @default.
• W4384927997 hasConceptScore W4384927997C2775988993 @default.
• W4384927997 hasConceptScore W4384927997C2776373379 @default.
• W4384927997 hasConceptScore W4384927997C55493867 @default.
• W4384927997 hasConceptScore W4384927997C59822182 @default.
• W4384927997 hasConceptScore W4384927997C69305403 @default.
• W4384927997 hasConceptScore W4384927997C86803240 @default.
• W4384927997 hasConceptScore W4384927997C93829228 @default.
• W4384927997 hasConceptScore W4384927997C95444343 @default.
• W4384927997 hasLocation W43849279971 @default.
• W4384927997 hasOpenAccess W4384927997 @default.
• W4384927997 hasPrimaryLocation W43849279971 @default.
• W4384927997 hasRelatedWork W1503200757 @default.
• W4384927997 hasRelatedWork W2015782946 @default.
• W4384927997 hasRelatedWork W2049568908 @default.
• W4384927997 hasRelatedWork W2068920848 @default.
• W4384927997 hasRelatedWork W2069947210 @default.
• W4384927997 hasRelatedWork W2085596002 @default.
• W4384927997 hasRelatedWork W2169336055 @default.

• next