Matches in SemOpenAlex for { <https://semopenalex.org/work/W4384940217> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4384940217 endingPage "9" @default.
- W4384940217 startingPage "1" @default.
- W4384940217 abstract "Background: Klebsiella pneumonia carbapenemases (blakpc) can hydrolyze carbapenems, which cause many bacteria resistance to multiple classes of antibiotics, so the rapid spread of blakpc is concerning. Laboratory identification of blakpc-carrying clinical isolates would be critical in limiting the bacteria's spread. This study would look at a quick and low-cost real-time PCR assay for detecting the level of expression of the black gene from Klebsiella pneumonia before and after being treated with a specific concentration of the Meropenemas drug. Method: From January 2021 to March 2021, Twenty-three K. pneumoniae strains were collected from patients presenting at various hospitals in Baghdad, Iraq. The disk diffusion method was used to test the strains, and the E-test minimum inhibitory concentration was determined (MIC). The black gene was then identified using the reverse transcription-PCR method. Finally, gene expression was measured using a real-time PCR assay in the presence and absence of Meropenem antibiotics. Results: Phenotypic testing revealed a high level of antibiotic resistance, whereas genotypic methods revealed the presence and expression of carbapenemase gene. Conclusion: The findings suggest revisions to current antibiotic therapy protocols due to the high expression level of resistant carbapenemases in K. pneumoniae strains. Keywords: MIC, Meropenemas antibiotic, Gene expression, Real-time PCR." @default.
- W4384940217 created "2023-07-22" @default.
- W4384940217 creator A5019075068 @default.
- W4384940217 creator A5036457344 @default.
- W4384940217 creator A5066614895 @default.
- W4384940217 date "2023-06-15" @default.
- W4384940217 modified "2023-10-17" @default.
- W4384940217 title "Molecular detection and gene expression of carbapenemase (blakpc) encoding gene of Klebsiella pneumonae isolated from clinical samples." @default.
- W4384940217 cites W2033235589 @default.
- W4384940217 cites W2065337857 @default.
- W4384940217 cites W2100494916 @default.
- W4384940217 cites W2104920080 @default.
- W4384940217 cites W2104996287 @default.
- W4384940217 cites W2107277218 @default.
- W4384940217 cites W2132963724 @default.
- W4384940217 cites W2134057459 @default.
- W4384940217 cites W214824510 @default.
- W4384940217 cites W2149088130 @default.
- W4384940217 cites W2419220498 @default.
- W4384940217 cites W2469153140 @default.
- W4384940217 cites W2754429943 @default.
- W4384940217 cites W3150587497 @default.
- W4384940217 cites W4230146414 @default.
- W4384940217 cites W4242904901 @default.
- W4384940217 doi "https://doi.org/10.21931/rb/2023.08.02.72" @default.
- W4384940217 hasPublicationYear "2023" @default.
- W4384940217 type Work @default.
- W4384940217 citedByCount "0" @default.
- W4384940217 crossrefType "journal-article" @default.
- W4384940217 hasAuthorship W4384940217A5019075068 @default.
- W4384940217 hasAuthorship W4384940217A5036457344 @default.
- W4384940217 hasAuthorship W4384940217A5066614895 @default.
- W4384940217 hasBestOaLocation W43849402171 @default.
- W4384940217 hasConcept C104317684 @default.
- W4384940217 hasConcept C114851261 @default.
- W4384940217 hasConcept C135763542 @default.
- W4384940217 hasConcept C150194340 @default.
- W4384940217 hasConcept C2776933286 @default.
- W4384940217 hasConcept C2777058267 @default.
- W4384940217 hasConcept C2779116282 @default.
- W4384940217 hasConcept C2779375183 @default.
- W4384940217 hasConcept C501593827 @default.
- W4384940217 hasConcept C523546767 @default.
- W4384940217 hasConcept C54355233 @default.
- W4384940217 hasConcept C547475151 @default.
- W4384940217 hasConcept C86803240 @default.
- W4384940217 hasConcept C89423630 @default.
- W4384940217 hasConcept C94665300 @default.
- W4384940217 hasConceptScore W4384940217C104317684 @default.
- W4384940217 hasConceptScore W4384940217C114851261 @default.
- W4384940217 hasConceptScore W4384940217C135763542 @default.
- W4384940217 hasConceptScore W4384940217C150194340 @default.
- W4384940217 hasConceptScore W4384940217C2776933286 @default.
- W4384940217 hasConceptScore W4384940217C2777058267 @default.
- W4384940217 hasConceptScore W4384940217C2779116282 @default.
- W4384940217 hasConceptScore W4384940217C2779375183 @default.
- W4384940217 hasConceptScore W4384940217C501593827 @default.
- W4384940217 hasConceptScore W4384940217C523546767 @default.
- W4384940217 hasConceptScore W4384940217C54355233 @default.
- W4384940217 hasConceptScore W4384940217C547475151 @default.
- W4384940217 hasConceptScore W4384940217C86803240 @default.
- W4384940217 hasConceptScore W4384940217C89423630 @default.
- W4384940217 hasConceptScore W4384940217C94665300 @default.
- W4384940217 hasIssue "2" @default.
- W4384940217 hasLocation W43849402171 @default.
- W4384940217 hasOpenAccess W4384940217 @default.
- W4384940217 hasPrimaryLocation W43849402171 @default.
- W4384940217 hasRelatedWork W2354813582 @default.
- W4384940217 hasRelatedWork W2358194747 @default.
- W4384940217 hasRelatedWork W2358306839 @default.
- W4384940217 hasRelatedWork W2364380996 @default.
- W4384940217 hasRelatedWork W2368926088 @default.
- W4384940217 hasRelatedWork W2376420536 @default.
- W4384940217 hasRelatedWork W2387160139 @default.
- W4384940217 hasRelatedWork W2761021459 @default.
- W4384940217 hasRelatedWork W2948065570 @default.
- W4384940217 hasRelatedWork W3138878599 @default.
- W4384940217 hasVolume "8" @default.
- W4384940217 isParatext "false" @default.
- W4384940217 isRetracted "false" @default.
- W4384940217 workType "article" @default.