Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385074032> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W4385074032 abstract "Background: Currently, the sex selection of cattle offspring holds great promise for genetic advancement and meeting consumer demand. Sperm sorting by flow cytometry provides a reliable tool for artificial insemination and the creation of embryos with predetermined sexes. A precise evaluation of the yield of sperm separation is still needed for a field application of this method or for the advancement and validation of other related semen sexing technologies. The current study was conducted to develop a qPCR-based method for the determination of sperm sex ratio in cow bull semen. Methods: The SYBR Green-based Real-Time PCR chemistry targeting PLP (X chromosome-specific gene) and SRY (Y chromosome-specific gene) genes were used for the development of the assay. To determine the efficiency of PCR amplification, standard curves were generated. Result: The developed assay revealed a negligible variation in the semen sex ratio (51.7±0.465% X and 48.23±0.465% Y) of unsorted semen. The repeatability and reproducibility of this approach were evaluated. For PLP and SRY, the standards produced a linear relationship with regression coefficients of 0.994 and 0.997, respectively. The low mean values of CV obtained in repeatability and reproducibility trials demonstrated the high dependability of this novel method for assessing the sexual chromosomal content in semen samples." @default.
- W4385074032 created "2023-07-23" @default.
- W4385074032 creator A5021307472 @default.
- W4385074032 creator A5046952748 @default.
- W4385074032 creator A5077450505 @default.
- W4385074032 creator A5092517951 @default.
- W4385074032 date "2023-07-22" @default.
- W4385074032 modified "2023-10-16" @default.
- W4385074032 title "Development of qPCR Assay for Determination of Sperm Sex Ratio in Indian Cow Bull" @default.
- W4385074032 doi "https://doi.org/10.18805/ijar.b-5047" @default.
- W4385074032 hasPublicationYear "2023" @default.
- W4385074032 type Work @default.
- W4385074032 citedByCount "0" @default.
- W4385074032 crossrefType "journal-article" @default.
- W4385074032 hasAuthorship W4385074032A5021307472 @default.
- W4385074032 hasAuthorship W4385074032A5046952748 @default.
- W4385074032 hasAuthorship W4385074032A5077450505 @default.
- W4385074032 hasAuthorship W4385074032A5092517951 @default.
- W4385074032 hasBestOaLocation W43850740321 @default.
- W4385074032 hasConcept C104317684 @default.
- W4385074032 hasConcept C154020017 @default.
- W4385074032 hasConcept C16685009 @default.
- W4385074032 hasConcept C179933525 @default.
- W4385074032 hasConcept C185592680 @default.
- W4385074032 hasConcept C196843134 @default.
- W4385074032 hasConcept C2778093475 @default.
- W4385074032 hasConcept C2779383911 @default.
- W4385074032 hasConcept C2780246931 @default.
- W4385074032 hasConcept C2781087480 @default.
- W4385074032 hasConcept C2994278967 @default.
- W4385074032 hasConcept C43617362 @default.
- W4385074032 hasConcept C54355233 @default.
- W4385074032 hasConcept C71924100 @default.
- W4385074032 hasConcept C73456138 @default.
- W4385074032 hasConcept C86803240 @default.
- W4385074032 hasConceptScore W4385074032C104317684 @default.
- W4385074032 hasConceptScore W4385074032C154020017 @default.
- W4385074032 hasConceptScore W4385074032C16685009 @default.
- W4385074032 hasConceptScore W4385074032C179933525 @default.
- W4385074032 hasConceptScore W4385074032C185592680 @default.
- W4385074032 hasConceptScore W4385074032C196843134 @default.
- W4385074032 hasConceptScore W4385074032C2778093475 @default.
- W4385074032 hasConceptScore W4385074032C2779383911 @default.
- W4385074032 hasConceptScore W4385074032C2780246931 @default.
- W4385074032 hasConceptScore W4385074032C2781087480 @default.
- W4385074032 hasConceptScore W4385074032C2994278967 @default.
- W4385074032 hasConceptScore W4385074032C43617362 @default.
- W4385074032 hasConceptScore W4385074032C54355233 @default.
- W4385074032 hasConceptScore W4385074032C71924100 @default.
- W4385074032 hasConceptScore W4385074032C73456138 @default.
- W4385074032 hasConceptScore W4385074032C86803240 @default.
- W4385074032 hasIssue "Of" @default.
- W4385074032 hasLocation W43850740321 @default.
- W4385074032 hasOpenAccess W4385074032 @default.
- W4385074032 hasPrimaryLocation W43850740321 @default.
- W4385074032 hasRelatedWork W1971405606 @default.
- W4385074032 hasRelatedWork W2009852724 @default.
- W4385074032 hasRelatedWork W2027646452 @default.
- W4385074032 hasRelatedWork W2115739634 @default.
- W4385074032 hasRelatedWork W2137193846 @default.
- W4385074032 hasRelatedWork W2371543766 @default.
- W4385074032 hasRelatedWork W2466813697 @default.
- W4385074032 hasRelatedWork W3190470638 @default.
- W4385074032 hasRelatedWork W4205934778 @default.
- W4385074032 hasRelatedWork W4285273795 @default.
- W4385074032 isParatext "false" @default.
- W4385074032 isRetracted "false" @default.
- W4385074032 workType "article" @default.