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- W4385142775 abstract "Abstract Ring-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened or trapped open and chaperoned onto DNA by clamp loader complexes (CLCs). Detailed understanding of the mechanisms by which CLCs open and place clamps around DNA remains limited. Here, we present a series of six structures of the Escherichia coli CLC bound to an open or closed clamp on and off a primer-template DNA that represent all intermediates in the clamp loading process. We show that the ATP-bound CLC first binds to a clamp, then constricts to hold onto it. The CLC then expands to open the clamp with a gap large enough for double-stranded DNA to enter. Upon binding to DNA, the CLC constricts slightly, allowing ATP hydrolysis and clamp closing around DNA. Although both yeast and E. coli CLCs open clamps by crab claw-like motions, they do it by the CLC expanding in opposite directions. These structures provide critical high-resolution snapshots of clamp loading by the E. coli CLC, revealing how the molecular machine works." @default.
- W4385142775 created "2023-07-23" @default.
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- W4385142775 date "2023-07-21" @default.
- W4385142775 modified "2023-09-27" @default.
- W4385142775 title "Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli" @default.
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- W4385142775 doi "https://doi.org/10.1101/2023.07.20.549978" @default.
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