Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385156521> ?p ?o ?g. }
- W4385156521 endingPage "209" @default.
- W4385156521 startingPage "198" @default.
- W4385156521 abstract "Spatial localizing of skeletal proteins in biogenic minerals remains a challenge in biomineralization research. To address this goal, we developed a novel in situ mapping technique based on molecular recognition measurements via atomic force microscopy (AFM), which requires three steps: (1) the development and purification of a polyclonal antibody elicited against the target protein, (2) its covalent coupling to a silicon nitride AFM tip ('functionalization'), and (3) scanning of an appropriately prepared biomineral surface. We applied this approach to a soluble shell protein - accripin11 - recently identified as a major component of the calcitic prisms of the fan mussel Pinna nobilis [1]. Multiple tests reveal that accripin11 is evenly distributed at the surface of the prisms and also present in the organic sheaths surrounding the calcitic prisms, indicating that this protein is both intra- and inter-crystalline. We observed that the adhesion force in transverse sections is about twice higher than in longitudinal sections, suggesting that accripin11 may exhibit preferred orientation in the biomineral. To our knowledge, this is the first time that a protein is localized by molecular recognition atomic force microscopy with antibody-functionalized tips in a biogenic mineral. The 'pros' and 'cons' of this methodology are discussed in comparison with more 'classical' approaches like immunogold. This technique, which leaves the surface to analyze clean, might prove useful for clinical tests on non-pathological (bone, teeth) or pathological (kidney stone) biomineralizations. Studies using implants with protein-doped calcium phosphate coating can also benefit from this technology. STATEMENT OF SIGNIFICANCE: Our paper deals with an unconventional technical approach for localizing proteins that are occluded in biominerals. This technique relies on the use of molecular recognition atomic force microscopy with antibody-functionalized tips. Although such approach has been employed in other system, this is the very first time that it is developed for biominerals. In comparison to more classical approaches (such as immunogold), AFM microscopy with antibody-functionalized tips allows higher magnification and keeps the scanned surface clean for other biophysical characterizations. Our method has a general scope as it can be applied in human health, for non-pathological (bone, teeth) and pathological (kidney stone) biomineralizations as well as for bone implants coated with protein-doped calcium phosphate." @default.
- W4385156521 created "2023-07-24" @default.
- W4385156521 creator A5001267475 @default.
- W4385156521 creator A5011009750 @default.
- W4385156521 creator A5023818977 @default.
- W4385156521 creator A5024468665 @default.
- W4385156521 creator A5026066548 @default.
- W4385156521 creator A5033513688 @default.
- W4385156521 creator A5063021617 @default.
- W4385156521 creator A5065404169 @default.
- W4385156521 creator A5065880113 @default.
- W4385156521 creator A5081033582 @default.
- W4385156521 creator A5085492458 @default.
- W4385156521 creator A5091501442 @default.
- W4385156521 date "2023-09-01" @default.
- W4385156521 modified "2023-10-08" @default.
- W4385156521 title "In situ mapping of biomineral skeletal proteins by molecular recognition imaging with antibody-functionalized AFM tips" @default.
- W4385156521 cites W1514282469 @default.
- W4385156521 cites W1534116117 @default.
- W4385156521 cites W1601969877 @default.
- W4385156521 cites W1801166380 @default.
- W4385156521 cites W1916531010 @default.
- W4385156521 cites W1932052686 @default.
- W4385156521 cites W1969212678 @default.
- W4385156521 cites W1969861754 @default.
- W4385156521 cites W1977225302 @default.
- W4385156521 cites W1980593939 @default.
- W4385156521 cites W1985189560 @default.
- W4385156521 cites W1985466994 @default.
- W4385156521 cites W1989149917 @default.
- W4385156521 cites W1993260194 @default.
- W4385156521 cites W1994649103 @default.
- W4385156521 cites W2002928212 @default.
- W4385156521 cites W2008534797 @default.
- W4385156521 cites W2014901449 @default.
- W4385156521 cites W2029019253 @default.
- W4385156521 cites W2030837016 @default.
- W4385156521 cites W2032641879 @default.
- W4385156521 cites W2033940741 @default.
- W4385156521 cites W2040013838 @default.
- W4385156521 cites W2040100879 @default.
- W4385156521 cites W2040510580 @default.
- W4385156521 cites W2043990263 @default.
- W4385156521 cites W2045328900 @default.
- W4385156521 cites W2051782550 @default.
- W4385156521 cites W2058485722 @default.
- W4385156521 cites W2058912578 @default.
- W4385156521 cites W2067516368 @default.
- W4385156521 cites W2067918294 @default.
- W4385156521 cites W2070391192 @default.
- W4385156521 cites W2072743055 @default.
- W4385156521 cites W2073277085 @default.
- W4385156521 cites W2081129368 @default.
- W4385156521 cites W2088534767 @default.
- W4385156521 cites W2089154372 @default.
- W4385156521 cites W2107618243 @default.
- W4385156521 cites W2113023067 @default.
- W4385156521 cites W2119460480 @default.
- W4385156521 cites W2153653432 @default.
- W4385156521 cites W2154733004 @default.
- W4385156521 cites W2159876084 @default.
- W4385156521 cites W2171413845 @default.
- W4385156521 cites W2257503224 @default.
- W4385156521 cites W2290457650 @default.
- W4385156521 cites W2326928761 @default.
- W4385156521 cites W2333491644 @default.
- W4385156521 cites W2461226865 @default.
- W4385156521 cites W2486025566 @default.
- W4385156521 cites W2510164746 @default.
- W4385156521 cites W2518820032 @default.
- W4385156521 cites W2552428206 @default.
- W4385156521 cites W2568203515 @default.
- W4385156521 cites W2739056564 @default.
- W4385156521 cites W2792918643 @default.
- W4385156521 cites W2955359014 @default.
- W4385156521 cites W3043975290 @default.
- W4385156521 cites W3045277044 @default.
- W4385156521 cites W3150338457 @default.
- W4385156521 cites W3163945002 @default.
- W4385156521 cites W3184870034 @default.
- W4385156521 cites W4304203412 @default.
- W4385156521 doi "https://doi.org/10.1016/j.actbio.2023.07.028" @default.
- W4385156521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37490960" @default.
- W4385156521 hasPublicationYear "2023" @default.
- W4385156521 type Work @default.
- W4385156521 citedByCount "0" @default.
- W4385156521 crossrefType "journal-article" @default.
- W4385156521 hasAuthorship W4385156521A5001267475 @default.
- W4385156521 hasAuthorship W4385156521A5011009750 @default.
- W4385156521 hasAuthorship W4385156521A5023818977 @default.
- W4385156521 hasAuthorship W4385156521A5024468665 @default.
- W4385156521 hasAuthorship W4385156521A5026066548 @default.
- W4385156521 hasAuthorship W4385156521A5033513688 @default.
- W4385156521 hasAuthorship W4385156521A5063021617 @default.
- W4385156521 hasAuthorship W4385156521A5065404169 @default.
- W4385156521 hasAuthorship W4385156521A5065880113 @default.
- W4385156521 hasAuthorship W4385156521A5081033582 @default.
- W4385156521 hasAuthorship W4385156521A5085492458 @default.