FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385158700> ?p ?o ?g. }

• W4385158700 abstract "1. Summary Chlamydia trachomatis (C. trachomatis) is a common sexually transmitted infection (STI). In 2019, the World Health Organization reported about 131 million infections. The majority of infected patients are asymptomatic with cases remaining undetected. It is likely that missed C. trachomatis infections contribute to preventable adverse health outcomes in women and children. Consequently, there is an urgent need of developing efficient diagnostic methods. In this study, genome-mining approaches to identify identical multi-repeat sequences (IMRS) distributed throughout the C. trachomatis genome were used to design a primer pair that would target regions in the genome. Genomic DNA was 10-fold serially diluted (100pg/μL to 1×10-3pg/μL) and used as DNA template for PCR reactions. The gold standard PCR using 16S rRNA primers was also run as a comparative test, and products were resolved on agarose gel. The novel assay, C. trachomatis IMRS-PCR, had an analytical sensitivity of 9.5 fg/μL, representing better sensitivity compared with 16S rRNA PCR (4.31 pg/μL). Our experimental data demonstrate the successful development of lateral flow and isothermal assays for detecting C. trachomatis DNA with potential use in field settings. There is a potential to implement this concept in miniaturized, isothermal, microfluidic platforms, and laboratory-on-a-chip diagnostic devices for reliable point-of-care testing." @default.
• W4385158700 created "2023-07-24" @default.
• W4385158700 creator A5000772668 @default.
• W4385158700 creator A5002132050 @default.
• W4385158700 creator A5005255971 @default.
• W4385158700 creator A5013867593 @default.
• W4385158700 creator A5023267458 @default.
• W4385158700 creator A5023621728 @default.
• W4385158700 creator A5031477287 @default.
• W4385158700 creator A5043061476 @default.
• W4385158700 creator A5056665544 @default.
• W4385158700 creator A5056924507 @default.
• W4385158700 creator A5063699921 @default.
• W4385158700 creator A5068914872 @default.
• W4385158700 creator A5069678363 @default.
• W4385158700 creator A5085544563 @default.
• W4385158700 creator A5092524816 @default.
• W4385158700 date "2023-07-23" @default.
• W4385158700 modified "2023-10-14" @default.
• W4385158700 title "Identification of Multi-repeat Sequences using Genome Mining Approaches for Developing Highly Sensitive Molecular Diagnostic Assay for the Detection of<i>Chlamydia trachomatis</i>" @default.
• W4385158700 cites W1972149072 @default.
• W4385158700 cites W2097907356 @default.
• W4385158700 cites W2112756031 @default.
• W4385158700 cites W2125076114 @default.
• W4385158700 cites W2129801045 @default.
• W4385158700 cites W2142185309 @default.
• W4385158700 cites W2144731787 @default.
• W4385158700 cites W2170942419 @default.
• W4385158700 cites W2479025411 @default.
• W4385158700 cites W2921118284 @default.
• W4385158700 cites W2937254574 @default.
• W4385158700 cites W2947693962 @default.
• W4385158700 cites W3003399548 @default.
• W4385158700 cites W3092150117 @default.
• W4385158700 cites W3174543614 @default.
• W4385158700 cites W4200480658 @default.
• W4385158700 cites W4214855071 @default.
• W4385158700 cites W4220782703 @default.
• W4385158700 cites W4323320061 @default.
• W4385158700 doi "https://doi.org/10.1101/2023.07.19.22272924" @default.
• W4385158700 hasPublicationYear "2023" @default.
• W4385158700 type Work @default.
• W4385158700 citedByCount "0" @default.
• W4385158700 crossrefType "posted-content" @default.
• W4385158700 hasAuthorship W4385158700A5000772668 @default.
• W4385158700 hasAuthorship W4385158700A5002132050 @default.
• W4385158700 hasAuthorship W4385158700A5005255971 @default.
• W4385158700 hasAuthorship W4385158700A5013867593 @default.
• W4385158700 hasAuthorship W4385158700A5023267458 @default.
• W4385158700 hasAuthorship W4385158700A5023621728 @default.
• W4385158700 hasAuthorship W4385158700A5031477287 @default.
• W4385158700 hasAuthorship W4385158700A5043061476 @default.
• W4385158700 hasAuthorship W4385158700A5056665544 @default.
• W4385158700 hasAuthorship W4385158700A5056924507 @default.
• W4385158700 hasAuthorship W4385158700A5063699921 @default.
• W4385158700 hasAuthorship W4385158700A5068914872 @default.
• W4385158700 hasAuthorship W4385158700A5069678363 @default.
• W4385158700 hasAuthorship W4385158700A5085544563 @default.
• W4385158700 hasAuthorship W4385158700A5092524816 @default.
• W4385158700 hasBestOaLocation W43851587001 @default.
• W4385158700 hasConcept C104317684 @default.
• W4385158700 hasConcept C126322002 @default.
• W4385158700 hasConcept C141231307 @default.
• W4385158700 hasConcept C159047783 @default.
• W4385158700 hasConcept C178790620 @default.
• W4385158700 hasConcept C185592680 @default.
• W4385158700 hasConcept C2777391075 @default.
• W4385158700 hasConcept C2777563447 @default.
• W4385158700 hasConcept C40993552 @default.
• W4385158700 hasConcept C49105822 @default.
• W4385158700 hasConcept C54355233 @default.
• W4385158700 hasConcept C552990157 @default.
• W4385158700 hasConcept C60635243 @default.
• W4385158700 hasConcept C70721500 @default.
• W4385158700 hasConcept C71924100 @default.
• W4385158700 hasConcept C86803240 @default.
• W4385158700 hasConceptScore W4385158700C104317684 @default.
• W4385158700 hasConceptScore W4385158700C126322002 @default.
• W4385158700 hasConceptScore W4385158700C141231307 @default.
• W4385158700 hasConceptScore W4385158700C159047783 @default.
• W4385158700 hasConceptScore W4385158700C178790620 @default.
• W4385158700 hasConceptScore W4385158700C185592680 @default.
• W4385158700 hasConceptScore W4385158700C2777391075 @default.
• W4385158700 hasConceptScore W4385158700C2777563447 @default.
• W4385158700 hasConceptScore W4385158700C40993552 @default.
• W4385158700 hasConceptScore W4385158700C49105822 @default.
• W4385158700 hasConceptScore W4385158700C54355233 @default.
• W4385158700 hasConceptScore W4385158700C552990157 @default.
• W4385158700 hasConceptScore W4385158700C60635243 @default.
• W4385158700 hasConceptScore W4385158700C70721500 @default.
• W4385158700 hasConceptScore W4385158700C71924100 @default.
• W4385158700 hasConceptScore W4385158700C86803240 @default.
• W4385158700 hasLocation W43851587001 @default.
• W4385158700 hasOpenAccess W4385158700 @default.
• W4385158700 hasPrimaryLocation W43851587001 @default.
• W4385158700 hasRelatedWork W1572434202 @default.
• W4385158700 hasRelatedWork W1875120346 @default.
• W4385158700 hasRelatedWork W1888003576 @default.
• W4385158700 hasRelatedWork W1994875126 @default.
• W4385158700 hasRelatedWork W2053464542 @default.

• next