FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385172693> ?p ?o ?g. }

• W4385172693 endingPage "11778" @default.
• W4385172693 startingPage "11778" @default.
• W4385172693 abstract "Let-7 is a tumor suppressor microRNA targeting the KRAS lung oncogene. Let-7a downregulation is reversible during the early stages of lung carcinogenesis but is irreversible in cancer cells. The aim of this study is to shed light on the relationship between oncogene (KRAS) mutation and let-7a downregulation in cigarette smoke (CS)-induced lung carcinogenesis.A total of 184 strain H Swiss albino mice were either unexposed (control) or exposed to CS for 2 weeks (short CS) or 8 months (long CS). After 8 months, the lungs were individually collected. The following end points have been evaluated: (a) DNA methylation of the let-7a gene promoter by bisulphite-PCR and pyrosequencing; (b) let-7a expression by qPCR; (c) KRAS mutation by DNA pyrosequencing; (d) cancer incidence by histopathological examination.let-7a expression decreased by 8.3% in the mice exposed to CS for two weeks (CS short) and by 33.4% (p ≤ 0.01) in the mice exposed to CS for 8 months (CS long). No significant difference was detected in the rate of let-7a-promoter methylation between the Sham-exposed mice (55.1%) and the CS short-(53%) or CS long (51%)-exposed mice. The percentage of G/T transversions in KRAS codons 12 and 13 increased from 2.3% (Sham) to 6.4% in CS short- and to 11.5% in CS long-exposed mice. Cancer incidence increased significantly in the CS long-exposed mice (11%) as compared to both the Sham (4%) and the CS short-exposed (2%) mice. In the CS long-exposed mice, the correlation between let-7a expression and the number of KRAS mutations was positive (R = +0.5506) in the cancer-free mice and negative (R = -0.5568) in the cancer-bearing mice.The effects of CS-induced mutations in KRAS are neutralized by the high expression of let-7a in cancer-free mice (positive correlation) but not in cancer-bearing mice where an irreversible let-7a downregulation occurs (negative correlation). This result provides evidence that both genetic (high load of KRAS mutation) and epigenetic alterations (let-7a irreversible downregulation) are required to produce lung cancer in CS-exposed organisms." @default.
• W4385172693 created "2023-07-24" @default.
• W4385172693 creator A5003475578 @default.
• W4385172693 creator A5007836073 @default.
• W4385172693 creator A5016255313 @default.
• W4385172693 creator A5060922137 @default.
• W4385172693 creator A5067745904 @default.
• W4385172693 creator A5089093466 @default.
• W4385172693 date "2023-07-21" @default.
• W4385172693 modified "2023-09-27" @default.
• W4385172693 title "Let-7a Downregulation Accompanied by KRAS Mutation Is Predictive of Lung Cancer Onset in Cigarette Smoke–Exposed Mice" @default.
• W4385172693 cites W1178842525 @default.
• W4385172693 cites W1504303645 @default.
• W4385172693 cites W1945014698 @default.
• W4385172693 cites W1973816411 @default.
• W4385172693 cites W1974218875 @default.
• W4385172693 cites W1977712961 @default.
• W4385172693 cites W1994225895 @default.
• W4385172693 cites W1999922074 @default.
• W4385172693 cites W2005098328 @default.
• W4385172693 cites W2011328027 @default.
• W4385172693 cites W2050654184 @default.
• W4385172693 cites W2068322460 @default.
• W4385172693 cites W2084133316 @default.
• W4385172693 cites W2084693974 @default.
• W4385172693 cites W2087911815 @default.
• W4385172693 cites W2093097047 @default.
• W4385172693 cites W2108402531 @default.
• W4385172693 cites W2113093407 @default.
• W4385172693 cites W2119363473 @default.
• W4385172693 cites W2120342857 @default.
• W4385172693 cites W2120722044 @default.
• W4385172693 cites W2131553001 @default.
• W4385172693 cites W2134354145 @default.
• W4385172693 cites W2135471644 @default.
• W4385172693 cites W2137649483 @default.
• W4385172693 cites W2144038313 @default.
• W4385172693 cites W2150726477 @default.
• W4385172693 cites W2155954232 @default.
• W4385172693 cites W2512013199 @default.
• W4385172693 cites W2531214390 @default.
• W4385172693 cites W2574524493 @default.
• W4385172693 cites W2593541846 @default.
• W4385172693 cites W2746973941 @default.
• W4385172693 cites W2790220405 @default.
• W4385172693 cites W2790801524 @default.
• W4385172693 cites W2892092697 @default.
• W4385172693 cites W2987555752 @default.
• W4385172693 cites W3000106795 @default.
• W4385172693 cites W3035177263 @default.
• W4385172693 cites W3092245147 @default.
• W4385172693 cites W3092351397 @default.
• W4385172693 cites W3133574869 @default.
• W4385172693 cites W4200565879 @default.
• W4385172693 cites W4214607842 @default.
• W4385172693 doi "https://doi.org/10.3390/ijms241411778" @default.
• W4385172693 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37511536" @default.
• W4385172693 hasPublicationYear "2023" @default.
• W4385172693 type Work @default.
• W4385172693 citedByCount "0" @default.
• W4385172693 crossrefType "journal-article" @default.
• W4385172693 hasAuthorship W4385172693A5003475578 @default.
• W4385172693 hasAuthorship W4385172693A5007836073 @default.
• W4385172693 hasAuthorship W4385172693A5016255313 @default.
• W4385172693 hasAuthorship W4385172693A5060922137 @default.
• W4385172693 hasAuthorship W4385172693A5067745904 @default.
• W4385172693 hasAuthorship W4385172693A5089093466 @default.
• W4385172693 hasBestOaLocation W43851726931 @default.
• W4385172693 hasConcept C104317684 @default.
• W4385172693 hasConcept C121608353 @default.
• W4385172693 hasConcept C125009961 @default.
• W4385172693 hasConcept C126322002 @default.
• W4385172693 hasConcept C127561419 @default.
• W4385172693 hasConcept C142724271 @default.
• W4385172693 hasConcept C150194340 @default.
• W4385172693 hasConcept C153911025 @default.
• W4385172693 hasConcept C16685009 @default.
• W4385172693 hasConcept C190727270 @default.
• W4385172693 hasConcept C2776256026 @default.
• W4385172693 hasConcept C2781018059 @default.
• W4385172693 hasConcept C2781187634 @default.
• W4385172693 hasConcept C29537977 @default.
• W4385172693 hasConcept C33288867 @default.
• W4385172693 hasConcept C502942594 @default.
• W4385172693 hasConcept C526805850 @default.
• W4385172693 hasConcept C54355233 @default.
• W4385172693 hasConcept C555283112 @default.
• W4385172693 hasConcept C71924100 @default.
• W4385172693 hasConcept C86803240 @default.
• W4385172693 hasConceptScore W4385172693C104317684 @default.
• W4385172693 hasConceptScore W4385172693C121608353 @default.
• W4385172693 hasConceptScore W4385172693C125009961 @default.
• W4385172693 hasConceptScore W4385172693C126322002 @default.
• W4385172693 hasConceptScore W4385172693C127561419 @default.
• W4385172693 hasConceptScore W4385172693C142724271 @default.
• W4385172693 hasConceptScore W4385172693C150194340 @default.
• W4385172693 hasConceptScore W4385172693C153911025 @default.
• W4385172693 hasConceptScore W4385172693C16685009 @default.

• next