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- W4385188207 abstract "Abstract The dopaminergic system has been extensively studied for its role in behavior in various animals as well as human neuropsychiatric and neurological diseases. However, we still know little about how dopamine levels are tightly regulated in vivo . In order to identify novel regulators of dopamine levels, we utilized Drosophila melanogaster cuticle pigmentation as a readout, where dopamine is used as a precursor to melanin. First, we measured dopamine from classical mutants of genes that are known to be important for cuticle pigmentation to understand the relationship between dopamine levels and cuticle color. We then performed an RNAi-based screen to identify novel regulators of cuticle pigmentation. We identified 153 genes that were not only enriched for conserved homologs and disease-associated genes, but were unexpectedly enriched for multiple developmental signaling pathways and mitochondria-associated proteins. Upon measuring dopamine from 35 prioritized candidates from this cohort, we found 10 that caused significant reduction in dopamine in the head while one caused an increase. While most of these 11 genes are expressed in the fly brain, only two of them, clu and mask, altered dopamine levels specifically in the brain upon knockdown in dopaminergic cells. Interestingly, mask may act as a hub of dopamine regulation since it is associated with three nodes found in our screen (Receptor tyrosine kinase/EGF signaling, Hippo signaling, and mitochondrial dynamics). Further examination suggests that Mask likely acts on dopamine synthesis by regulating transcription of the rate-limiting dopamine synthesis enzyme, tyrosine hydroxylase . In conclusion, this screen identified 11 new regulators of dopamine levels and provides molecular handles to investigate how dopamine levels are controlled in vivo , as well as revealed an unexpected relationship between fly pigmentation genes, developmental signaling and human neurological and neurodevelopmental disease genes." @default.
- W4385188207 created "2023-07-25" @default.
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- W4385188207 date "2023-07-24" @default.
- W4385188207 modified "2023-09-27" @default.
- W4385188207 title "RNAi-based screen for cuticle pigmentation in<i>Drosophila melanogaster</i>reveals novel regulators of brain dopamine" @default.
- W4385188207 cites W1482306551 @default.
- W4385188207 cites W1497290376 @default.
- W4385188207 cites W1538557493 @default.
- W4385188207 cites W1601034297 @default.
- W4385188207 cites W1877873625 @default.
- W4385188207 cites W1964188110 @default.
- W4385188207 cites W1966282260 @default.
- W4385188207 cites W1969645371 @default.
- W4385188207 cites W1969800514 @default.
- W4385188207 cites W1973324928 @default.
- W4385188207 cites W1973617706 @default.
- W4385188207 cites W1976557090 @default.
- W4385188207 cites W1981605166 @default.
- W4385188207 cites W1982917739 @default.
- W4385188207 cites W1984304489 @default.
- W4385188207 cites W1988040227 @default.
- W4385188207 cites W1991835460 @default.
- W4385188207 cites W2008830477 @default.
- W4385188207 cites W2010747656 @default.
- W4385188207 cites W2013085811 @default.
- W4385188207 cites W2015652178 @default.
- W4385188207 cites W2018887368 @default.
- W4385188207 cites W2027505886 @default.
- W4385188207 cites W2029985957 @default.
- W4385188207 cites W2030714603 @default.
- W4385188207 cites W2031718641 @default.
- W4385188207 cites W2032576926 @default.
- W4385188207 cites W2033716417 @default.
- W4385188207 cites W2034949400 @default.
- W4385188207 cites W2035505151 @default.
- W4385188207 cites W2036338354 @default.
- W4385188207 cites W2041063545 @default.
- W4385188207 cites W2041330970 @default.
- W4385188207 cites W2043203446 @default.
- W4385188207 cites W2055665256 @default.
- W4385188207 cites W2061148723 @default.
- W4385188207 cites W2063628404 @default.
- W4385188207 cites W2075873978 @default.
- W4385188207 cites W2077260129 @default.
- W4385188207 cites W2081356991 @default.
- W4385188207 cites W2082874836 @default.
- W4385188207 cites W2086477923 @default.
- W4385188207 cites W2087197800 @default.
- W4385188207 cites W2090817340 @default.
- W4385188207 cites W2092098765 @default.
- W4385188207 cites W2095817446 @default.
- W4385188207 cites W2096131346 @default.
- W4385188207 cites W2096454034 @default.
- W4385188207 cites W2098931453 @default.
- W4385188207 cites W2103046819 @default.
- W4385188207 cites W2104024903 @default.
- W4385188207 cites W2105039688 @default.
- W4385188207 cites W2108249916 @default.
- W4385188207 cites W2109963281 @default.
- W4385188207 cites W2110643873 @default.
- W4385188207 cites W2112616760 @default.
- W4385188207 cites W2114431124 @default.
- W4385188207 cites W2116214216 @default.
- W4385188207 cites W2117236995 @default.
- W4385188207 cites W2118810801 @default.
- W4385188207 cites W2122826962 @default.
- W4385188207 cites W2123515098 @default.
- W4385188207 cites W2130487306 @default.
- W4385188207 cites W2136388422 @default.
- W4385188207 cites W2142667969 @default.
- W4385188207 cites W2143161560 @default.
- W4385188207 cites W2145506897 @default.
- W4385188207 cites W2150609468 @default.
- W4385188207 cites W2150972545 @default.
- W4385188207 cites W2151104570 @default.
- W4385188207 cites W2154457951 @default.
- W4385188207 cites W2155540878 @default.
- W4385188207 cites W2156455641 @default.
- W4385188207 cites W2157096314 @default.
- W4385188207 cites W2165454389 @default.
- W4385188207 cites W2168149778 @default.
- W4385188207 cites W2168950294 @default.
- W4385188207 cites W2253563636 @default.
- W4385188207 cites W2262549635 @default.
- W4385188207 cites W2291066974 @default.
- W4385188207 cites W2293565794 @default.
- W4385188207 cites W2341971745 @default.
- W4385188207 cites W2342539172 @default.
- W4385188207 cites W2411013142 @default.
- W4385188207 cites W2533524158 @default.
- W4385188207 cites W2536273977 @default.
- W4385188207 cites W2586689382 @default.