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• W4385192096 endingPage "136997" @default.
• W4385192096 startingPage "136997" @default.
• W4385192096 abstract "We report the fabrication of a facile sensor using heme conjugated with gold nanoparticles (AuNPs) in situ on a glass carbon electrode (GCE) for the ultrasensitive determination of biotin without antibody or streptavidin. The use of heme and AuNPs as dual amplifiers allows a very broad detection range from 0.0050 to 50.0000 μmol·L-1 and a very low detection limit of 0.0016 μmol·L-1. The mechanistic aspects were elucidated using electrochemical analyses and frontier orbital calculations showing that the electrooxidation of biotin involves a one-electron and a one-proton transfer, generating biotin sulfoxide. The heme/AuNPs/GCE sensor exhibited excellent selectivity, reproducibility and stability, indicating high robustness. The recovery was between 97.20 and 105.70% with RSD less than 8.71%, suggesting good practicability. Our studies demonstrate that this approach can be used to detect and quantify biotin in a range of foods, including milk, infant formula, flour, orange juice, mango juice, egg white and egg yolk. Furthermore, all measurements do not require any intricate preparation or pre-treatment of the foods, thus representing a great potential for point-of-care testing." @default.
• W4385192096 created "2023-07-25" @default.
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• W4385192096 date "2023-12-01" @default.
• W4385192096 modified "2023-10-14" @default.
• W4385192096 title "Electrochemical sensor for ultrasensitive sensing of biotin based on heme conjugated with gold nanoparticles and its electrooxidation mechanism" @default.
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• W4385192096 doi "https://doi.org/10.1016/j.foodchem.2023.136997" @default.
• W4385192096 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37516051" @default.
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