Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385217534> ?p ?o ?g. }
- W4385217534 abstract "Abstract As a response to invasion by pathogens, the secretion of interleukin 6 (IL-6) which is a cytokine, activates IL-6/JAKs/STAT3 intracellular signaling via., phosphorylation. Over expression of pSTAT3 induces IL-6 positive feedback loop causing cytokine release syndrome or cytokine storm. Plants have gained momentum as an alternative expression system. Hence, this study aims to produce mAb targeting human IL-6 receptor (hIL-6R) in Nicotiana benthamiana for down regulating its cellular signaling thus, decreasing the expression of pSTAT3. The variable regions of heavy and light chains of anti-hIL-6R mAb were constructed in pBYK2e geminiviral plant expression vector and transiently co-expressed in N. benthamiana . The results demonstrate the proper protein assembly of anti-hIL-6R mAb with highest expression level of 2.24 mg/g FW at 5 dpi, with a yield of 21.4 µg/g FW after purification. The purity and N-glycosylation of plant produced antibody was analyzed, including its specificity to human IL-6 receptor by ELISA. Additionally, we investigated the effect to pSTAT3 expression in human PBMC’s by flow cytometry wherein, the results confirmed lower expression of pSTAT3 with increasing concentrations of plant produced anti-hIL-6R mAb. Although, further in vivo studies are key to unveil the absolute functionality of anti-hIL-6R, we hereby show the potential of the plant platform and its suitability for the production of this therapeutic antibody." @default.
- W4385217534 created "2023-07-25" @default.
- W4385217534 creator A5000023773 @default.
- W4385217534 creator A5003422586 @default.
- W4385217534 creator A5010190025 @default.
- W4385217534 creator A5012639078 @default.
- W4385217534 creator A5023102968 @default.
- W4385217534 creator A5029443484 @default.
- W4385217534 creator A5036923506 @default.
- W4385217534 creator A5049878186 @default.
- W4385217534 creator A5083895647 @default.
- W4385217534 creator A5092533834 @default.
- W4385217534 date "2023-07-24" @default.
- W4385217534 modified "2023-09-23" @default.
- W4385217534 title "Effect of plant produced Anti-hIL-6 receptor antibody blockade on pSTAT3 expression in human peripheral blood mononuclear cells" @default.
- W4385217534 cites W1489022916 @default.
- W4385217534 cites W1678784904 @default.
- W4385217534 cites W1776476655 @default.
- W4385217534 cites W1858549592 @default.
- W4385217534 cites W1895277992 @default.
- W4385217534 cites W1908675203 @default.
- W4385217534 cites W1973687921 @default.
- W4385217534 cites W1983416604 @default.
- W4385217534 cites W1987192197 @default.
- W4385217534 cites W2000707284 @default.
- W4385217534 cites W2010818113 @default.
- W4385217534 cites W2030380228 @default.
- W4385217534 cites W2031432627 @default.
- W4385217534 cites W2036564735 @default.
- W4385217534 cites W2054915407 @default.
- W4385217534 cites W2062006145 @default.
- W4385217534 cites W2069724685 @default.
- W4385217534 cites W2074702359 @default.
- W4385217534 cites W2078382205 @default.
- W4385217534 cites W2103548696 @default.
- W4385217534 cites W2121558572 @default.
- W4385217534 cites W2125933228 @default.
- W4385217534 cites W2134160733 @default.
- W4385217534 cites W2143670089 @default.
- W4385217534 cites W2147946725 @default.
- W4385217534 cites W2156826212 @default.
- W4385217534 cites W2161483639 @default.
- W4385217534 cites W2175291039 @default.
- W4385217534 cites W2276779069 @default.
- W4385217534 cites W2308447553 @default.
- W4385217534 cites W2406851765 @default.
- W4385217534 cites W2535550742 @default.
- W4385217534 cites W2592079742 @default.
- W4385217534 cites W2593700382 @default.
- W4385217534 cites W2765166506 @default.
- W4385217534 cites W2786987313 @default.
- W4385217534 cites W2793477763 @default.
- W4385217534 cites W2793753066 @default.
- W4385217534 cites W2803430447 @default.
- W4385217534 cites W2901043781 @default.
- W4385217534 cites W2909080906 @default.
- W4385217534 cites W2914918388 @default.
- W4385217534 cites W2948719773 @default.
- W4385217534 cites W2949709502 @default.
- W4385217534 cites W2952260713 @default.
- W4385217534 cites W2974794559 @default.
- W4385217534 cites W2986168648 @default.
- W4385217534 cites W2992472283 @default.
- W4385217534 cites W2998036497 @default.
- W4385217534 cites W3014050022 @default.
- W4385217534 cites W3014527183 @default.
- W4385217534 cites W3022970339 @default.
- W4385217534 cites W3038883715 @default.
- W4385217534 cites W3081790187 @default.
- W4385217534 cites W3087721486 @default.
- W4385217534 cites W3088889602 @default.
- W4385217534 cites W3091455200 @default.
- W4385217534 cites W3093426014 @default.
- W4385217534 cites W3108094105 @default.
- W4385217534 cites W3110844195 @default.
- W4385217534 cites W3129362092 @default.
- W4385217534 cites W3129397954 @default.
- W4385217534 cites W3136497524 @default.
- W4385217534 cites W3158895710 @default.
- W4385217534 cites W3175467526 @default.
- W4385217534 cites W3176652851 @default.
- W4385217534 cites W3186049690 @default.
- W4385217534 cites W3198780266 @default.
- W4385217534 cites W3198955675 @default.
- W4385217534 cites W3202138311 @default.
- W4385217534 cites W3207474548 @default.
- W4385217534 cites W3208123091 @default.
- W4385217534 cites W3209791546 @default.
- W4385217534 cites W3216383335 @default.
- W4385217534 cites W4200108102 @default.
- W4385217534 cites W4213447223 @default.
- W4385217534 cites W4254038388 @default.
- W4385217534 cites W4282929793 @default.
- W4385217534 cites W4308791755 @default.
- W4385217534 cites W4311102730 @default.
- W4385217534 cites W4317521338 @default.
- W4385217534 cites W4366247278 @default.
- W4385217534 cites W750942006 @default.
- W4385217534 doi "https://doi.org/10.1038/s41598-023-39106-5" @default.
- W4385217534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37488213" @default.