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- W4385234384 abstract "As a chemical platform, 2,3-Butanediol (2,3-BD) has been widely applied in various industrial fields. In this study, to enhance the production of 2,3-BD by Saccharomyces cerevisiae L7, Plackett–Burman (PB) multifactorial design, the steepest climb test and central composite design (CCD) were employed to optimize the culturing conditions of S. cerevisiae L7. The results show that acetic acid, peptone and glucose were contributing factors for 2,3-BD production. Subsequently, a satisfactory production of 2,3-BD (13.52 ± 0.12 g/L) was reached under optimal conditions, which was 3.12 times higher than before optimization. Furthermore, fed-batch fermentation was carried out under optimized culture conditions, and a higher production and yield efficiency of 2,3-BD were achieved (21.83 ± 0.56 g/L and 0.15 ± 0.01 g/g, respectively) when glucose (20 g/L) and acetic acid (0.2 g/L) were added at 12, 24, 36, 48 and 60 h. Therefore, the production and yield efficiency of 2,3-BD were higher than those without fed-batch fermentation (61.46% and 58.51%, respectively). These results provide good support and a technical foundation for the large-scale industrial production of 2,3-BD by Saccharomyces cerevisiae." @default.
- W4385234384 created "2023-07-26" @default.
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- W4385234384 date "2023-07-24" @default.
- W4385234384 modified "2023-10-14" @default.
- W4385234384 title "Production of 2,3-Butanediol by S. cerevisiae L7 in Fed-Batch Fermentation with Optimized Culture Conditions" @default.
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- W4385234384 doi "https://doi.org/10.3390/fermentation9070694" @default.
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