Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385238975> ?p ?o ?g. }
- W4385238975 abstract "During development, cortical (c) and medullary (m) thymic epithelial cells (TEC) arise from the third pharyngeal pouch endoderm. Current models suggest that within the thymic primordium most TEC exist in a bipotent/common thymic epithelial progenitor cell (TEPC) state able to generate both cTEC and mTEC, at least until embryonic day 12.5 (E12.5) in the mouse. This view, however, is challenged by recent transcriptomics and genetic evidence. We therefore set out to investigate the fate and potency of TEC in the early thymus. Here using single cell (sc) RNAseq we identify a candidate mTEC progenitor population at E12.5, consistent with recent reports. Via lineage-tracing we demonstrate this population as mTEC fate-restricted, validating our bioinformatics prediction. Using potency analyses we also establish that most E11.5 and E12.5 progenitor TEC are cTEC-fated. Finally we show that overnight culture causes most if not all E12.5 cTEC-fated TEPC to acquire functional bipotency, and provide a likely molecular mechanism for this changed differentiation potential. Collectively, our data overturn the widely held view that a common TEPC predominates in the E12.5 thymus, showing instead that sublineage-primed progenitors are present from the earliest stages of thymus organogenesis but that these early fetal TEPC exhibit cell-fate plasticity in response to extrinsic factors. Our data provide a significant advance in the understanding of fetal thymic epithelial development and thus have implications for thymus-related clinical research, in particular research focussed on generating TEC from pluripotent stem cells." @default.
- W4385238975 created "2023-07-26" @default.
- W4385238975 creator A5008565592 @default.
- W4385238975 creator A5011641448 @default.
- W4385238975 creator A5021435190 @default.
- W4385238975 creator A5028138093 @default.
- W4385238975 creator A5036611727 @default.
- W4385238975 creator A5037539904 @default.
- W4385238975 creator A5042571447 @default.
- W4385238975 creator A5043489990 @default.
- W4385238975 creator A5046020733 @default.
- W4385238975 creator A5052223241 @default.
- W4385238975 creator A5055719742 @default.
- W4385238975 creator A5065926799 @default.
- W4385238975 creator A5072042710 @default.
- W4385238975 creator A5072509653 @default.
- W4385238975 creator A5072934932 @default.
- W4385238975 creator A5081265499 @default.
- W4385238975 creator A5081545907 @default.
- W4385238975 date "2023-07-25" @default.
- W4385238975 modified "2023-09-26" @default.
- W4385238975 title "Thymic epithelial cell fate and potency in early organogenesis assessed by single cell transcriptional and functional analysis" @default.
- W4385238975 cites W1503136384 @default.
- W4385238975 cites W1573828236 @default.
- W4385238975 cites W1575564659 @default.
- W4385238975 cites W1583729352 @default.
- W4385238975 cites W1939607990 @default.
- W4385238975 cites W1964974221 @default.
- W4385238975 cites W1967399128 @default.
- W4385238975 cites W1969275538 @default.
- W4385238975 cites W1972263413 @default.
- W4385238975 cites W1980896849 @default.
- W4385238975 cites W1981362835 @default.
- W4385238975 cites W1982569530 @default.
- W4385238975 cites W1986557797 @default.
- W4385238975 cites W1988666643 @default.
- W4385238975 cites W1992856202 @default.
- W4385238975 cites W1993183180 @default.
- W4385238975 cites W1993405888 @default.
- W4385238975 cites W1995790934 @default.
- W4385238975 cites W1999928832 @default.
- W4385238975 cites W2004754687 @default.
- W4385238975 cites W2008356343 @default.
- W4385238975 cites W2015966979 @default.
- W4385238975 cites W2018718597 @default.
- W4385238975 cites W2024748688 @default.
- W4385238975 cites W2025605686 @default.
- W4385238975 cites W2026875813 @default.
- W4385238975 cites W2029487548 @default.
- W4385238975 cites W2029665585 @default.
- W4385238975 cites W2033488512 @default.
- W4385238975 cites W2033810895 @default.
- W4385238975 cites W2037572029 @default.
- W4385238975 cites W2038558025 @default.
- W4385238975 cites W2039000125 @default.
- W4385238975 cites W2039877884 @default.
- W4385238975 cites W2046118442 @default.
- W4385238975 cites W2050543129 @default.
- W4385238975 cites W2050972863 @default.
- W4385238975 cites W2060357276 @default.
- W4385238975 cites W2061965920 @default.
- W4385238975 cites W2068495472 @default.
- W4385238975 cites W2075134069 @default.
- W4385238975 cites W2104135721 @default.
- W4385238975 cites W2113320664 @default.
- W4385238975 cites W2114768669 @default.
- W4385238975 cites W2121088412 @default.
- W4385238975 cites W2121413434 @default.
- W4385238975 cites W2125679885 @default.
- W4385238975 cites W2134526812 @default.
- W4385238975 cites W2136086257 @default.
- W4385238975 cites W2139419405 @default.
- W4385238975 cites W2141778874 @default.
- W4385238975 cites W2145520919 @default.
- W4385238975 cites W2146588354 @default.
- W4385238975 cites W2146671387 @default.
- W4385238975 cites W2147871659 @default.
- W4385238975 cites W2150328261 @default.
- W4385238975 cites W2162058869 @default.
- W4385238975 cites W2165214298 @default.
- W4385238975 cites W2169456326 @default.
- W4385238975 cites W2184716885 @default.
- W4385238975 cites W2257197315 @default.
- W4385238975 cites W2294951120 @default.
- W4385238975 cites W2295870578 @default.
- W4385238975 cites W2299956396 @default.
- W4385238975 cites W2302374603 @default.
- W4385238975 cites W2338028476 @default.
- W4385238975 cites W2388244934 @default.
- W4385238975 cites W2461577324 @default.
- W4385238975 cites W2518245007 @default.
- W4385238975 cites W2527088177 @default.
- W4385238975 cites W2557186815 @default.
- W4385238975 cites W2616922646 @default.
- W4385238975 cites W3030176903 @default.
- W4385238975 cites W3031266716 @default.
- W4385238975 cites W3152361137 @default.
- W4385238975 cites W4206956815 @default.
- W4385238975 cites W4213096223 @default.
- W4385238975 cites W4281488532 @default.