FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385255552> ?p ?o ?g. }

• W4385255552 endingPage "11860" @default.
• W4385255552 startingPage "11860" @default.
• W4385255552 abstract "Here, we present evidence that caveolae-mediated endocytosis using LDLR is the pathway for SARS-CoV-2 virus internalization in the ocular cell line ARPE-19. Firstly, we found that, while Angiotensin-converting enzyme 2 (ACE2) is expressed in these cells, blocking ACE2 by antibody treatment did not prevent infection by SARS-CoV-2 spike pseudovirions, nor did antibody blockade of extracellular vimentin and other cholesterol-rich lipid raft proteins. Next, we implicated the role of cholesterol homeostasis in infection by showing that incubating cells with different cyclodextrins and oxysterol 25-hydroxycholesterol (25-HC) inhibits pseudovirion infection of ARPE-19. However, the effect of 25-HC is likely not via cholesterol biosynthesis, as incubation with lovastatin did not appreciably affect infection. Additionally, is it not likely to be an agonistic effect of 25-HC on LXR receptors, as the LXR agonist GW3965 had no significant effect on infection of ARPE-19 cells at up to 5 μM GW3965. We probed the role of endocytic pathways but determined that clathrin-dependent and flotillin-dependent rafts were not involved. Furthermore, 20 µM chlorpromazine, an inhibitor of clathrin-mediated endocytosis (CME), also had little effect. In contrast, anti-dynamin I/II antibodies blocked the entry of SARS-CoV-2 spike pseudovirions, as did dynasore, a noncompetitive inhibitor of dynamin GTPase activity. Additionally, anti-caveolin-1 antibodies significantly blocked spike pseudotyped lentiviral infection of ARPE-19. However, nystatin, a classic inhibitor of caveolae-dependent endocytosis, did not affect infection while indomethacin inhibited only at 10 µM at the 48 h time point. Finally, we found that anti-LDLR antibodies block pseudovirion infection to a similar degree as anti-caveolin-1 and anti-dynamin I/II antibodies, while transfection with LDLR-specific siRNA led to a decrease in spike pseudotyped lentiviral infection, compared to scrambled control siRNAs. Thus, we conclude that SARS-CoV-2 spike pseudovirion infection in ARPE-19 cells is a dynamin-dependent process that is primarily mediated by LDLR." @default.
• W4385255552 created "2023-07-26" @default.
• W4385255552 creator A5011127352 @default.
• W4385255552 creator A5011845016 @default.
• W4385255552 creator A5020986917 @default.
• W4385255552 creator A5022819280 @default.
• W4385255552 creator A5032376253 @default.
• W4385255552 creator A5037182958 @default.
• W4385255552 creator A5064197056 @default.
• W4385255552 creator A5078435172 @default.
• W4385255552 date "2023-07-24" @default.
• W4385255552 modified "2023-10-18" @default.
• W4385255552 title "Low-Density Lipoprotein Receptor (LDLR) Is Involved in Internalization of Lentiviral Particles Pseudotyped with SARS-CoV-2 Spike Protein in Ocular Cells" @default.
• W4385255552 cites W1568412294 @default.
• W4385255552 cites W1990985479 @default.
• W4385255552 cites W1998477311 @default.
• W4385255552 cites W2000469457 @default.
• W4385255552 cites W2021877458 @default.
• W4385255552 cites W2026269865 @default.
• W4385255552 cites W2036802778 @default.
• W4385255552 cites W2047393488 @default.
• W4385255552 cites W2068851900 @default.
• W4385255552 cites W2080261305 @default.
• W4385255552 cites W2083626146 @default.
• W4385255552 cites W2084744450 @default.
• W4385255552 cites W2105671322 @default.
• W4385255552 cites W2110245801 @default.
• W4385255552 cites W2113504004 @default.
• W4385255552 cites W2118982822 @default.
• W4385255552 cites W2136847298 @default.
• W4385255552 cites W2144206275 @default.
• W4385255552 cites W2145190710 @default.
• W4385255552 cites W2153317225 @default.
• W4385255552 cites W2157159684 @default.
• W4385255552 cites W2402467387 @default.
• W4385255552 cites W2471349002 @default.
• W4385255552 cites W2564200748 @default.
• W4385255552 cites W2605167569 @default.
• W4385255552 cites W2766963286 @default.
• W4385255552 cites W2809969369 @default.
• W4385255552 cites W2890544210 @default.
• W4385255552 cites W3004280078 @default.
• W4385255552 cites W3009335299 @default.
• W4385255552 cites W3009912996 @default.
• W4385255552 cites W3010441732 @default.
• W4385255552 cites W3012883269 @default.
• W4385255552 cites W3015190630 @default.
• W4385255552 cites W3022873918 @default.
• W4385255552 cites W3025226065 @default.
• W4385255552 cites W3031684671 @default.
• W4385255552 cites W3036529319 @default.
• W4385255552 cites W3038889249 @default.
• W4385255552 cites W3045080797 @default.
• W4385255552 cites W3048642124 @default.
• W4385255552 cites W3059166130 @default.
• W4385255552 cites W3081371429 @default.
• W4385255552 cites W3081498413 @default.
• W4385255552 cites W3092290045 @default.
• W4385255552 cites W3093118962 @default.
• W4385255552 cites W3093867667 @default.
• W4385255552 cites W3094419073 @default.
• W4385255552 cites W3107588714 @default.
• W4385255552 cites W3118861857 @default.
• W4385255552 cites W3120758145 @default.
• W4385255552 cites W3121790907 @default.
• W4385255552 cites W3128504046 @default.
• W4385255552 cites W3134027938 @default.
• W4385255552 cites W3135511673 @default.
• W4385255552 cites W3156389344 @default.
• W4385255552 cites W3160989313 @default.
• W4385255552 cites W3168276412 @default.
• W4385255552 cites W3168333544 @default.
• W4385255552 cites W3185950833 @default.
• W4385255552 cites W4200211352 @default.
• W4385255552 cites W4206957604 @default.
• W4385255552 cites W4210595038 @default.
• W4385255552 cites W4210787373 @default.
• W4385255552 cites W4220667558 @default.
• W4385255552 cites W4296807072 @default.
• W4385255552 cites W4367838604 @default.
• W4385255552 doi "https://doi.org/10.3390/ijms241411860" @default.
• W4385255552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37511618" @default.
• W4385255552 hasPublicationYear "2023" @default.
• W4385255552 type Work @default.
• W4385255552 citedByCount "0" @default.
• W4385255552 crossrefType "journal-article" @default.
• W4385255552 hasAuthorship W4385255552A5011127352 @default.
• W4385255552 hasAuthorship W4385255552A5011845016 @default.
• W4385255552 hasAuthorship W4385255552A5020986917 @default.
• W4385255552 hasAuthorship W4385255552A5022819280 @default.
• W4385255552 hasAuthorship W4385255552A5032376253 @default.
• W4385255552 hasAuthorship W4385255552A5037182958 @default.
• W4385255552 hasAuthorship W4385255552A5064197056 @default.
• W4385255552 hasAuthorship W4385255552A5078435172 @default.
• W4385255552 hasBestOaLocation W43852555521 @default.
• W4385255552 hasConcept C139770010 @default.
• W4385255552 hasConcept C140704245 @default.
• W4385255552 hasConcept C156407911 @default.

• next