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- W4385269436 endingPage "11960" @default.
- W4385269436 startingPage "11960" @default.
- W4385269436 abstract "The yeast S. cerevisiae is a unique genetic object for which a wide range of relatively simple, inexpensive, and non-time-consuming methods have been developed that allow the performing of a wide variety of genome modifications. Among the latter, one can mention point mutations, disruptions and deletions of particular genes and regions of chromosomes, insertion of cassettes for the expression of heterologous genes, targeted chromosomal rearrangements such as translocations and inversions, directed changes in the karyotype (loss or duplication of particular chromosomes, changes in the level of ploidy), mating-type changes, etc. Classical yeast genome manipulations have been advanced with CRISPR/Cas9 technology in recent years that allow for the generation of multiple simultaneous changes in the yeast genome. In this review we discuss practical applications of both the classical yeast genome modification methods as well as CRISPR/Cas9 technology. In addition, we review methods for ploidy changes, including aneuploid generation, methods for mating type switching and directed DSB. Combined with a description of useful selective markers and transformation techniques, this work represents a nearly complete guide to yeast genome modification." @default.
- W4385269436 created "2023-07-27" @default.
- W4385269436 creator A5003521011 @default.
- W4385269436 creator A5005895283 @default.
- W4385269436 creator A5082301465 @default.
- W4385269436 creator A5092545260 @default.
- W4385269436 date "2023-07-26" @default.
- W4385269436 modified "2023-10-18" @default.
- W4385269436 title "Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification" @default.
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