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- W4385273041 abstract "Abstract For unveiling pathological evolution of breast cancer, nonlinear multiphoton microscopic (MPM) and confocal Raman microspectral imaging (CRMI) techniques were both utilized to address the structural and constitutional characteristics of healthy (H), ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC) tissues. MPM-based techniques, including two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), visualized label-free and high- the fine structure of breast tissue. Meanwhile, CRMI not only presented the chemical images of investigated samples with K-mean cluster analysis method (KCA), but also pictured the distribution of components in the scanned area through univariate imaging. MPM images illustrated that the cancer cells first arranged around the basement membrane of the duct, then proliferated to fill the lumens of the duct, and finally broken through the basement membrane to infiltrate into the stroma. Although the Raman imaging failed to visualize the cell structure with high resolution, it explained spectroscopically the gradual increase of nucleic acid and protein components inside the ducts as cancer cells proliferated, and displayed the distribution pattern of each biological component during the evolution of breast cancer. Thus, the combination of MPM and CRMI provides new insights into the on-site pathological diagnosis of malignant breast cancer, but also ensures technical support for the development of multimodal optical imaging techniques for precise histopathological analysis." @default.
- W4385273041 created "2023-07-27" @default.
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- W4385273041 date "2023-07-26" @default.
- W4385273041 modified "2023-10-04" @default.
- W4385273041 title "Assessing pathological features of breast cancer via the multimodal information of multiphoton and Raman imaging" @default.
- W4385273041 doi "https://doi.org/10.1088/1674-1056/acea67" @default.
- W4385273041 hasPublicationYear "2023" @default.
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