Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385280302> ?p ?o ?g. }
- W4385280302 abstract "Abstract Yarrowia lipolytica is an alternative yeast for heterologous protein production. Based on auto-cloning vectors, a set of 18 chromogenic cloning vectors was developed, each containing one of the excisable auxotrophic selective markers URA3ex, LYS5ex, and LEU2ex, and one of six different promoters: the constitutive pTEF, the phase dependent hybrid pHp4d, and the erythritol-inducible promoters from pEYK1 and pEYL1 derivatives. These vectors allowed to increase the speed of cloning of the gene of interest. In parallel, an improved new rProt recipient strain JMY8647 was developed by abolishing filamentation and introducing an auxotrophy for lysine (Lys−), providing an additional marker for genetic engineering. Using this cloning strategy, the optimal targeting sequence for Rhizopus oryzae ROL lipase secretion was determined. Among the eight targeting sequences, the SP6 signal sequence resulted in a 23% improvement in the lipase activity compared to that obtained with the wild-type ROL signal sequence. Higher specific lipase activities were obtained using hybrid erythritol-inducible promoters pHU8EYK and pEYL1-5AB, 1.9 and 2.2 times, respectively, when compared with the constitutive pTEF promoter. Two copy strains produce a 3.3 fold increase in lipase activity over the pTEF monocopy strain (266.7 versus 79.7 mU/mg)." @default.
- W4385280302 created "2023-07-27" @default.
- W4385280302 creator A5008739991 @default.
- W4385280302 creator A5027322092 @default.
- W4385280302 creator A5033042329 @default.
- W4385280302 creator A5044442534 @default.
- W4385280302 creator A5070015005 @default.
- W4385280302 date "2023-01-01" @default.
- W4385280302 modified "2023-09-27" @default.
- W4385280302 title "Production of <i>Rhizopus oryzae</i> lipase using optimized <i>Yarrowia lipolytica</i> expression system" @default.
- W4385280302 cites W1595115988 @default.
- W4385280302 cites W1843633333 @default.
- W4385280302 cites W1932509535 @default.
- W4385280302 cites W1972810808 @default.
- W4385280302 cites W1977141777 @default.
- W4385280302 cites W1985304367 @default.
- W4385280302 cites W1997755204 @default.
- W4385280302 cites W2004603941 @default.
- W4385280302 cites W2015503169 @default.
- W4385280302 cites W2018289835 @default.
- W4385280302 cites W2039790529 @default.
- W4385280302 cites W2049965804 @default.
- W4385280302 cites W2063862686 @default.
- W4385280302 cites W2070876179 @default.
- W4385280302 cites W2074180965 @default.
- W4385280302 cites W2079013207 @default.
- W4385280302 cites W2079039425 @default.
- W4385280302 cites W2086176935 @default.
- W4385280302 cites W2086267477 @default.
- W4385280302 cites W2089312229 @default.
- W4385280302 cites W2100837269 @default.
- W4385280302 cites W2129345131 @default.
- W4385280302 cites W2149227156 @default.
- W4385280302 cites W2151235959 @default.
- W4385280302 cites W2162094072 @default.
- W4385280302 cites W2165360475 @default.
- W4385280302 cites W2168581753 @default.
- W4385280302 cites W2521913447 @default.
- W4385280302 cites W2584173879 @default.
- W4385280302 cites W2588337104 @default.
- W4385280302 cites W2589018863 @default.
- W4385280302 cites W2626998764 @default.
- W4385280302 cites W2747790759 @default.
- W4385280302 cites W2800730019 @default.
- W4385280302 cites W2893777397 @default.
- W4385280302 cites W2898027229 @default.
- W4385280302 cites W2912719568 @default.
- W4385280302 cites W2979744467 @default.
- W4385280302 cites W3002407239 @default.
- W4385280302 cites W3004063674 @default.
- W4385280302 cites W3014952872 @default.
- W4385280302 cites W3168229649 @default.
- W4385280302 cites W4220826627 @default.
- W4385280302 cites W4283522746 @default.
- W4385280302 cites W428954129 @default.
- W4385280302 cites W4293168917 @default.
- W4385280302 cites W4293528089 @default.
- W4385280302 cites W4307265867 @default.
- W4385280302 cites W4315781604 @default.
- W4385280302 doi "https://doi.org/10.1093/femsyr/foad037" @default.
- W4385280302 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37496194" @default.
- W4385280302 hasPublicationYear "2023" @default.
- W4385280302 type Work @default.
- W4385280302 citedByCount "0" @default.
- W4385280302 crossrefType "journal-article" @default.
- W4385280302 hasAuthorship W4385280302A5008739991 @default.
- W4385280302 hasAuthorship W4385280302A5027322092 @default.
- W4385280302 hasAuthorship W4385280302A5033042329 @default.
- W4385280302 hasAuthorship W4385280302A5044442534 @default.
- W4385280302 hasAuthorship W4385280302A5070015005 @default.
- W4385280302 hasBestOaLocation W43852803021 @default.
- W4385280302 hasConcept C100544194 @default.
- W4385280302 hasConcept C101762097 @default.
- W4385280302 hasConcept C104317684 @default.
- W4385280302 hasConcept C10858879 @default.
- W4385280302 hasConcept C121050878 @default.
- W4385280302 hasConcept C150194340 @default.
- W4385280302 hasConcept C153911025 @default.
- W4385280302 hasConcept C181199279 @default.
- W4385280302 hasConcept C199360897 @default.
- W4385280302 hasConcept C2778541762 @default.
- W4385280302 hasConcept C2779490540 @default.
- W4385280302 hasConcept C2779697368 @default.
- W4385280302 hasConcept C40767141 @default.
- W4385280302 hasConcept C41008148 @default.
- W4385280302 hasConcept C54355233 @default.
- W4385280302 hasConcept C55493867 @default.
- W4385280302 hasConcept C86803240 @default.
- W4385280302 hasConceptScore W4385280302C100544194 @default.
- W4385280302 hasConceptScore W4385280302C101762097 @default.
- W4385280302 hasConceptScore W4385280302C104317684 @default.
- W4385280302 hasConceptScore W4385280302C10858879 @default.
- W4385280302 hasConceptScore W4385280302C121050878 @default.
- W4385280302 hasConceptScore W4385280302C150194340 @default.
- W4385280302 hasConceptScore W4385280302C153911025 @default.
- W4385280302 hasConceptScore W4385280302C181199279 @default.
- W4385280302 hasConceptScore W4385280302C199360897 @default.
- W4385280302 hasConceptScore W4385280302C2778541762 @default.
- W4385280302 hasConceptScore W4385280302C2779490540 @default.
- W4385280302 hasConceptScore W4385280302C2779697368 @default.