Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385294969> ?p ?o ?g. }
- W4385294969 abstract "Zebrafish exhibit a robust ability to regenerate their hearts following injury, and the immune system plays a key role in this process. We previously showed that delaying macrophage recruitment by clodronate liposome (-1d_CL, macrophage-delayed model) impairs neutrophil resolution and heart regeneration, even when the infiltrating macrophage number was restored within the first week post injury (Lai et al., 2017). It is thus intriguing to learn the regenerative macrophage property by comparing these late macrophages vs. control macrophages during cardiac repair. Here, we further investigate the mechanistic insights of heart regeneration by comparing the non-regenerative macrophage-delayed model with regenerative controls. Temporal RNAseq analyses revealed that -1d_CL treatment led to disrupted inflammatory resolution, reactive oxygen species homeostasis, and energy metabolism during cardiac repair. Comparative single-cell RNAseq profiling of inflammatory cells from regenerative vs. non-regenerative hearts further identified heterogeneous macrophages and neutrophils, showing alternative activation and cellular crosstalk leading to neutrophil retention and chronic inflammation. Among macrophages, two residential subpopulations (hbaa+ Mac and timp4.3+ Mac 3) were enriched only in regenerative hearts and barely recovered after +1d_CL treatment. To deplete the resident macrophage without delaying the circulating macrophage recruitment, we established the resident macrophage-deficient model by administrating CL earlier at 8 d (-8d_CL) before cryoinjury. Strikingly, resident macrophage-deficient zebrafish still exhibited defects in revascularization, cardiomyocyte survival, debris clearance, and extracellular matrix remodeling/scar resolution without functional compensation from the circulating/monocyte-derived macrophages. Our results characterized the diverse function and interaction between inflammatory cells and identified unique resident macrophages prerequisite for zebrafish heart regeneration." @default.
- W4385294969 created "2023-07-28" @default.
- W4385294969 creator A5002328948 @default.
- W4385294969 creator A5016489406 @default.
- W4385294969 creator A5019373437 @default.
- W4385294969 creator A5025418311 @default.
- W4385294969 creator A5026092126 @default.
- W4385294969 creator A5060372094 @default.
- W4385294969 creator A5073077400 @default.
- W4385294969 creator A5079162272 @default.
- W4385294969 creator A5087109225 @default.
- W4385294969 date "2023-07-27" @default.
- W4385294969 modified "2023-10-16" @default.
- W4385294969 title "Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration" @default.
- W4385294969 cites W1016059894 @default.
- W4385294969 cites W1480590796 @default.
- W4385294969 cites W1501940501 @default.
- W4385294969 cites W1526435001 @default.
- W4385294969 cites W1532126421 @default.
- W4385294969 cites W1577637355 @default.
- W4385294969 cites W1898569975 @default.
- W4385294969 cites W1964457547 @default.
- W4385294969 cites W1966202908 @default.
- W4385294969 cites W1966586109 @default.
- W4385294969 cites W1969038150 @default.
- W4385294969 cites W1980548655 @default.
- W4385294969 cites W1981044204 @default.
- W4385294969 cites W1983581444 @default.
- W4385294969 cites W1984883254 @default.
- W4385294969 cites W1988694095 @default.
- W4385294969 cites W2000729312 @default.
- W4385294969 cites W2001567020 @default.
- W4385294969 cites W2002132236 @default.
- W4385294969 cites W2002958873 @default.
- W4385294969 cites W2003406245 @default.
- W4385294969 cites W2005400104 @default.
- W4385294969 cites W2007631412 @default.
- W4385294969 cites W2010484682 @default.
- W4385294969 cites W2011170801 @default.
- W4385294969 cites W2014693052 @default.
- W4385294969 cites W2016845287 @default.
- W4385294969 cites W2020179710 @default.
- W4385294969 cites W2022287530 @default.
- W4385294969 cites W2035005072 @default.
- W4385294969 cites W2042433863 @default.
- W4385294969 cites W2052644234 @default.
- W4385294969 cites W2055327656 @default.
- W4385294969 cites W2058544221 @default.
- W4385294969 cites W2066279279 @default.
- W4385294969 cites W2074401088 @default.
- W4385294969 cites W2083311984 @default.
- W4385294969 cites W2091760375 @default.
- W4385294969 cites W2095472825 @default.
- W4385294969 cites W2096240415 @default.
- W4385294969 cites W2099548299 @default.
- W4385294969 cites W2102174943 @default.
- W4385294969 cites W2103018087 @default.
- W4385294969 cites W2104005232 @default.
- W4385294969 cites W2109771497 @default.
- W4385294969 cites W2109892435 @default.
- W4385294969 cites W2109951379 @default.
- W4385294969 cites W2111472409 @default.
- W4385294969 cites W2112125919 @default.
- W4385294969 cites W2112528263 @default.
- W4385294969 cites W2118680196 @default.
- W4385294969 cites W2126956583 @default.
- W4385294969 cites W2127274965 @default.
- W4385294969 cites W2140367720 @default.
- W4385294969 cites W2147466370 @default.
- W4385294969 cites W2152533590 @default.
- W4385294969 cites W2154811651 @default.
- W4385294969 cites W2159675211 @default.
- W4385294969 cites W2159875818 @default.
- W4385294969 cites W2161575454 @default.
- W4385294969 cites W2162143298 @default.
- W4385294969 cites W2162767010 @default.
- W4385294969 cites W2169686109 @default.
- W4385294969 cites W2170396482 @default.
- W4385294969 cites W2170841568 @default.
- W4385294969 cites W2190545194 @default.
- W4385294969 cites W2191859395 @default.
- W4385294969 cites W2287797653 @default.
- W4385294969 cites W2341478304 @default.
- W4385294969 cites W2491831296 @default.
- W4385294969 cites W2516344353 @default.
- W4385294969 cites W2521944793 @default.
- W4385294969 cites W2557577256 @default.
- W4385294969 cites W2571725398 @default.
- W4385294969 cites W2594292781 @default.
- W4385294969 cites W2697082363 @default.
- W4385294969 cites W2763257724 @default.
- W4385294969 cites W2763341318 @default.
- W4385294969 cites W2792681620 @default.
- W4385294969 cites W2794480084 @default.
- W4385294969 cites W2795137969 @default.
- W4385294969 cites W2796022005 @default.
- W4385294969 cites W2797066573 @default.
- W4385294969 cites W2890926420 @default.
- W4385294969 cites W2902050841 @default.
- W4385294969 cites W2902652978 @default.